UNC13_ARATH
ID UNC13_ARATH Reviewed; 1101 AA.
AC Q8RX56; Q9FL49;
DT 28-MAR-2018, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 121.
DE RecName: Full=Protein unc-13 homolog {ECO:0000305};
DE AltName: Full=Protein PROTON ATPASE TRANSLOCATION CONTROL 1 {ECO:0000303|PubMed:23896897};
GN Name=PATROL1 {ECO:0000303|PubMed:23896897};
GN OrderedLocusNames=At5g06970 {ECO:0000312|Araport:AT5G06970};
GN ORFNames=MOJ9.14 {ECO:0000312|EMBL:BAB11155.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9679202; DOI=10.1093/dnares/5.2.131;
RA Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence
RT features of the regions of 1,381,565 bp covered by twenty one physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:131-145(1998).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DISRUPTION
RP PHENOTYPE.
RX PubMed=23896897; DOI=10.1038/ncomms3215;
RA Hashimoto-Sugimoto M., Higaki T., Yaeno T., Nagami A., Irie M., Fujimi M.,
RA Miyamoto M., Akita K., Negi J., Shirasu K., Hasezawa S., Iba K.;
RT "A Munc13-like protein in Arabidopsis mediates H+-ATPase translocation that
RT is essential for stomatal responses.";
RL Nat. Commun. 4:2215-2215(2013).
CC -!- FUNCTION: Controls the tethering of the proton ATPase AHA1 to the
CC plasma membrane. Is essential for stomatal opening in response to low
CC concentration of carbon dioxide and light.
CC {ECO:0000269|PubMed:23896897}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:23896897}. Cell
CC membrane {ECO:0000269|PubMed:23896897}; Peripheral membrane protein
CC {ECO:0000305}. Note=Translocates reversibly between the cytoplasm and
CC the plasma membrane depending on environmental conditions.
CC {ECO:0000269|PubMed:23896897}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, cotyledons, leaves, stems and
CC flowers. Expressed in guard cells and mesophyll cells of leaves.
CC {ECO:0000269|PubMed:23896897}.
CC -!- DISRUPTION PHENOTYPE: Retarded growth due to impaired stomatal
CC movement. {ECO:0000269|PubMed:23896897}.
CC -!- SIMILARITY: Belongs to the unc-13 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB11155.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB010697; BAB11155.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED91091.1; -; Genomic_DNA.
DR EMBL; AY090450; AAL91294.1; -; mRNA.
DR EMBL; BT002724; AAO11640.1; -; mRNA.
DR RefSeq; NP_196314.2; NM_120779.3.
DR AlphaFoldDB; Q8RX56; -.
DR IntAct; Q8RX56; 2.
DR iPTMnet; Q8RX56; -.
DR PaxDb; Q8RX56; -.
DR PRIDE; Q8RX56; -.
DR ProteomicsDB; 245273; -.
DR EnsemblPlants; AT5G06970.1; AT5G06970.1; AT5G06970.
DR GeneID; 830588; -.
DR Gramene; AT5G06970.1; AT5G06970.1; AT5G06970.
DR KEGG; ath:AT5G06970; -.
DR Araport; AT5G06970; -.
DR TAIR; locus:2169424; AT5G06970.
DR eggNOG; ENOG502QT32; Eukaryota.
DR HOGENOM; CLU_009468_2_0_1; -.
DR InParanoid; Q8RX56; -.
DR OMA; TETIHHT; -.
DR OrthoDB; 1399846at2759; -.
DR PhylomeDB; Q8RX56; -.
DR PRO; PR:Q8RX56; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8RX56; baseline and differential.
DR GO; GO:0005737; C:cytoplasm; IDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0072659; P:protein localization to plasma membrane; IMP:TAIR.
DR GO; GO:0010118; P:stomatal movement; IMP:TAIR.
DR InterPro; IPR014770; Munc13_1.
DR InterPro; IPR014772; Munc13_dom-2.
DR InterPro; IPR008528; unc-13_homologue.
DR PANTHER; PTHR31280; PTHR31280; 1.
DR PROSITE; PS51258; MHD1; 1.
DR PROSITE; PS51259; MHD2; 1.
PE 2: Evidence at transcript level;
KW Cell membrane; Cytoplasm; Membrane; Reference proteome.
FT CHAIN 1..1101
FT /note="Protein unc-13 homolog"
FT /id="PRO_0000443727"
FT DOMAIN 663..804
FT /note="MHD1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00587"
FT DOMAIN 941..1051
FT /note="MHD2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00588"
SQ SEQUENCE 1101 AA; 124523 MW; DB4D7A5B16131811 CRC64;
MEEENAVEIL QRYRRDRRKL LDFMLAGSLI KKVIMPPGAV TLDDVDLDQV SVDYVINCAK
KGGMLELAEA IRDYHDHIGL PYMNSVGTAD EFFLATIPES SGSPPKRAPP PIPVLISSSS
PMVTNPEWCE SPSAPPLMRS ESFDSPKAQE LTVDDIDDFE DDDDLDEVGN FRISRRTAND
AADLVPRLPS FATGITDDDL RETAFEILLA CAGASGGLIV PSKEKKKEKS RSRLIKKLGR
KSESVSQSQS SSGLVSLLEM MRGQMEISEA MDIRTRQGLL NALAGKVGKR MDSLLVPLEL
LCCVSRTEFS DKKAYLRWQK RQLNMLAEGL INNPVVGFGE SGRKATDLKS LLLRIEESES
LPSSAGEVQR AECLKSLREV AISLAERPAR GDLTGEVCHW ADGYHLNVRL YEKLLLCVFD
ILNDGKLTEE VEEILELLKS TWRVLGITET IHYTCYAWVL FRQYVITSER GLLRHAIQQL
KKIPLKEQRG PQERLHLKTL KCRVDNEEIS FLESFLSPIR SWADKQLGDY HLHFAEGSLV
MEDTVTVAMI TWRLLLEESD RAMHSNSSDR EQIESYVLSS IKNTFTRMSL AIDRSDRNNE
HHLALLAEET KKLMKKDSTI FMPILSQRHP QAIAFSASLI HKLYGNKLKP FLDGAEHLTE
DAVSVFPAAD SLEQYLLELM TSVCGEDTSG PYFKKLIPYE VESLSGTLVL RWINSQLGRI
LSWVERAYKQ EHWDPISPQQ RYGSSIVEVF RIVEETVDQF FALKVPMRSI ELSALFRGID
NAFQVYTNHV MEKLASKDDL VPPVPVLTRY KKETAIKVFV KKELFDSKHL DERRSINIDV
PATAMLCVQL NTLHYAVSQL SKLEDSMWLR WIAKKPREKI VIRKSMVEKS KSFNQKESFE
GSRKDINAAL DRICEFTGTK IIFCDLREPF IENLYKPNVS QSRLEGLIEA LDTELGQLCS
VIMEPLRDRI VTSLLQASLD GLLRVLLDGG ASRVFHPSES KLLEEDVEVL KEFFISGGDG
LPRGVVENQV ARVRLVVKLH GYETRELIDD LRSRSSLEMQ QGGKGKLGAD TQTLVRVLCH
RNDSEASQFL KKQYKIPRSH G
