UNC3_CAEEL
ID UNC3_CAEEL Reviewed; 491 AA.
AC Q93705; K8FE05; O61576;
DT 30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT 27-MAY-2002, sequence version 3.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Transcription factor unc-3;
DE AltName: Full=Uncoordinated protein 3;
DE Short=CEO/E;
GN Name=unc-3; ORFNames=Y16B4A.1;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=Bristol N2;
RX PubMed=9502737; DOI=10.1242/dev.125.8.1561;
RA Prasad B.C., Ye B., Zackhary R., Schrader K., Seydoux G., Reed R.R.;
RT "unc-3, a gene required for axonal guidance in Caenorhabditis elegans,
RT encodes a member of the O/E family of transcription factors.";
RL Development 125:1561-1568(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND INTERACTION WITH JMJD-3.1.
RX PubMed=25124442; DOI=10.1126/science.1255885;
RA Zuryn S., Ahier A., Portoso M., White E.R., Morin M.C., Margueron R.,
RA Jarriault S.;
RT "Sequential histone-modifying activities determine the robustness of
RT transdifferentiation.";
RL Science 345:826-829(2014).
RN [4]
RP FUNCTION, AND MUTAGENESIS OF 309-TRP--SER-491.
RX PubMed=28056346; DOI=10.1016/j.neuron.2016.11.036;
RA Kerk S.Y., Kratsios P., Hart M., Mourao R., Hobert O.;
RT "Diversification of C. elegans Motor Neuron Identity via Selective Effector
RT Gene Repression.";
RL Neuron 93:80-98(2017).
CC -!- FUNCTION: Transcription factor (PubMed:28056346). Involved in motor
CC neuron fate determination and maintenance, acting as an activator of
CC gene expression in a subset of motor neurons (PubMed:28056346).
CC Required to maintain the expression of transcriptional repressors bnc-1
CC and cfi-1, which play roles in the cell fate of motor neurons
CC (PubMed:28056346). May play a role in the expression of proteins
CC essential for axonal pathfinding and/or neuronal differentiation in
CC both sensory and motor neurons (PubMed:9502737). Cooperates with jmjd-
CC 3.1 and wdr-5.1 to ensure robust transdifferentiation of the Y rectal
CC cell to the PDA motor neuron during larval development
CC (PubMed:25124442). {ECO:0000269|PubMed:25124442,
CC ECO:0000269|PubMed:28056346, ECO:0000269|PubMed:9502737}.
CC -!- SUBUNIT: Interacts with jmjd-3.1. {ECO:0000269|PubMed:25124442}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC -!- DEVELOPMENTAL STAGE: Expressed in certain chemosensory neurons (ASI
CC amphid neurons) throughout development and is also expressed
CC transiently in developing motor neurons when these cells undergo axonal
CC outgrowth. {ECO:0000269|PubMed:9502737}.
CC -!- DISRUPTION PHENOTYPE: Worms show defects in the axonal outgrowth of
CC motor neurons, as well as defects in dauer formation, a process
CC requiring chemosensory inputs. {ECO:0000269|PubMed:9502737}.
CC -!- SIMILARITY: Belongs to the COE family. {ECO:0000305}.
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DR EMBL; AF052060; AAC06226.1; -; mRNA.
DR EMBL; AL023825; CCO25892.1; -; Genomic_DNA.
DR EMBL; Z81081; CCO25892.1; JOINED; Genomic_DNA.
DR PIR; T22089; T22089.
DR PIR; T42991; T42991.
DR RefSeq; NP_510453.1; NM_078052.3.
DR AlphaFoldDB; Q93705; -.
DR SMR; Q93705; -.
DR BioGRID; 46470; 6.
DR DIP; DIP-61432N; -.
DR IntAct; Q93705; 1.
DR STRING; 6239.Y16B4A.1; -.
DR EPD; Q93705; -.
DR PaxDb; Q93705; -.
DR EnsemblMetazoa; Y16B4A.1.1; Y16B4A.1.1; WBGene00006743.
DR GeneID; 181573; -.
DR KEGG; cel:CELE_Y16B4A.1; -.
DR UCSC; Y16B4A.1; c. elegans.
DR CTD; 181573; -.
DR WormBase; Y16B4A.1; CE29366; WBGene00006743; unc-3.
DR eggNOG; KOG3836; Eukaryota.
DR GeneTree; ENSGT00950000182859; -.
DR HOGENOM; CLU_016320_0_0_1; -.
DR InParanoid; Q93705; -.
DR OMA; LCPSEGW; -.
DR OrthoDB; 817293at2759; -.
DR PhylomeDB; Q93705; -.
DR PRO; PR:Q93705; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00006743; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0035035; F:histone acetyltransferase binding; IPI:WormBase.
DR GO; GO:0042802; F:identical protein binding; IDA:WormBase.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR GO; GO:0007411; P:axon guidance; IMP:UniProtKB.
DR GO; GO:0040024; P:dauer larval development; IGI:WormBase.
DR GO; GO:0043065; P:positive regulation of apoptotic process; IMP:WormBase.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IMP:WormBase.
DR GO; GO:0010975; P:regulation of neuron projection development; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR CDD; cd11606; COE_DBD; 1.
DR CDD; cd01175; IPT_COE; 1.
DR Gene3D; 2.60.40.10; -; 1.
DR Gene3D; 2.60.40.3180; -; 1.
DR InterPro; IPR032200; COE_DBD.
DR InterPro; IPR038173; COE_DBD_sf.
DR InterPro; IPR032201; COE_HLH.
DR InterPro; IPR038006; COE_IPT.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR002909; IPT_dom.
DR InterPro; IPR003523; Transcription_factor_COE.
DR InterPro; IPR018350; Transcription_factor_COE_CS.
DR PANTHER; PTHR10747; PTHR10747; 1.
DR Pfam; PF16422; COE1_DBD; 1.
DR Pfam; PF16423; COE1_HLH; 1.
DR Pfam; PF01833; TIG; 1.
DR SMART; SM00429; IPT; 1.
DR SUPFAM; SSF81296; SSF81296; 1.
DR PROSITE; PS01345; COE; 1.
PE 1: Evidence at protein level;
KW Developmental protein; DNA-binding; Metal-binding; Nucleus;
KW Reference proteome; Transcription; Transcription regulation; Zinc;
KW Zinc-finger.
FT CHAIN 1..491
FT /note="Transcription factor unc-3"
FT /id="PRO_0000107824"
FT DOMAIN 269..355
FT /note="IPT/TIG"
FT /evidence="ECO:0000255"
FT ZN_FING 154..173
FT /note="C5-type"
FT /evidence="ECO:0000255"
FT REGION 66..69
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250|UniProtKB:Q07802"
FT REGION 200..207
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250|UniProtKB:Q07802"
FT REGION 239..242
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250|UniProtKB:Q07802"
FT REGION 240..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT SITE 166
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250|UniProtKB:Q07802"
FT SITE 175
FT /note="Interaction with DNA"
FT /evidence="ECO:0000250|UniProtKB:Q07802"
FT MUTAGEN 309..491
FT /note="Missing: In e151; drastically reduces expression of
FT acetylcholine receptor genes acr-5 and acr-16. In a bnc-1
FT mutant background, abolishes expression of the BMP-like
FT protein unc-129 in DA and DB motor neurons (MNs);
FT furthermore, ectopic expression of unc-129 in VA and VB MNs
FT is also lost. In a mab-9 mutant background, ectopic
FT expression of Degenerin del-1 gene in DA and DB MNs is
FT abolished."
FT /evidence="ECO:0000269|PubMed:28056346"
SQ SEQUENCE 491 AA; 55001 MW; CFC71C132D02D485 CRC64;
MSLTAPLRAG QMNFYDEPYN PVLNLHIQPS VKDENQRSTW PIIDTSNTST QIARAHFEKH
PPNNLRKSNF FHFVIALYDR NSQPIEVERT QFAGFVEKEK EVDGQDTRNG IHYRLSLMFQ
NGIRSEHDLF VRLIDSSTKQ AITYEGQDKN PEMCRVLLTH EVMCSRCCEK KSCGNRNETP
SDPVIIDRFF LKFFLKCNQN CLKNAGNPRD MRRFQVVLCS SARIDGPLLA VSDNMFVHNN
SKHGRRTKRT DASDDSEYSE SAELPSSVPV IKALFPSEGW IQGGTQVVLI GENFFEGLQV
AFGTASPNWG ESVQLISPHA IRVTTPPKHS AGPVDVTLQY KSKTYSRGTP LRFSYITLAE
PGIEYGFQRL QKLLPKYPGD PERLPKDQIL KRAAELAEAL YNRTSTESLS SYYHTQFDAT
SDYAARTHTS PRSTLPYGAG PPALSSAVYQ TSYPTVNATP AANFLNTQTG FATFGAVNPF
AATLQSSSRL S
