UNC47_CAEEL
ID UNC47_CAEEL Reviewed; 486 AA.
AC P34579; O17475;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 11-JUL-2001, sequence version 2.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Vesicular GABA transporter;
DE AltName: Full=Uncoordinated protein 47;
DE Short=Protein unc-47;
GN Name=unc-47; ORFNames=T20G5.6;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, AND MUTAGENESIS
RP OF GLY-462.
RX PubMed=9349821; DOI=10.1038/39908;
RA McIntire S.L., Reimer R.J., Schuske K., Edwards R.H., Jorgensen E.M.;
RT "Identification and characterization of the vesicular GABA transporter.";
RL Nature 389:870-876(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
CC -!- FUNCTION: Involved in the uptake of GABA into the synaptic vesicles.
CC {ECO:0000269|PubMed:9349821}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC {ECO:0000269|PubMed:9349821}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:9349821}.
CC -!- SIMILARITY: Belongs to the amino acid/polyamine transporter 2 family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF031935; AAB87066.1; -; mRNA.
DR EMBL; Z30423; CAA83006.2; -; Genomic_DNA.
DR PIR; S42372; S42372.
DR PIR; T42254; T42254.
DR RefSeq; NP_499255.1; NM_066854.5.
DR AlphaFoldDB; P34579; -.
DR SMR; P34579; -.
DR STRING; 6239.T20G5.6.1; -.
DR TCDB; 2.A.18.5.1; the amino acid/auxin permease (aaap) family.
DR PaxDb; P34579; -.
DR EnsemblMetazoa; T20G5.6.1; T20G5.6.1; WBGene00006783.
DR GeneID; 176431; -.
DR KEGG; cel:CELE_T20G5.6; -.
DR UCSC; T20G5.6.2; c. elegans.
DR CTD; 176431; -.
DR WormBase; T20G5.6; CE25119; WBGene00006783; unc-47.
DR eggNOG; KOG4303; Eukaryota.
DR GeneTree; ENSGT00940000168583; -.
DR HOGENOM; CLU_036432_0_0_1; -.
DR InParanoid; P34579; -.
DR OMA; KTFFQDG; -.
DR OrthoDB; 570025at2759; -.
DR PhylomeDB; P34579; -.
DR Reactome; R-CEL-425393; Transport of inorganic cations/anions and amino acids/oligopeptides.
DR Reactome; R-CEL-888590; GABA synthesis, release, reuptake and degradation.
DR PRO; PR:P34579; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00006783; Expressed in larva and 3 other tissues.
DR GO; GO:0044292; C:dendrite terminus; IBA:GO_Central.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0044306; C:neuron projection terminus; IBA:GO_Central.
DR GO; GO:0008021; C:synaptic vesicle; IDA:WormBase.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015495; F:gamma-aminobutyric acid:proton symporter activity; IBA:GO_Central.
DR GO; GO:0015187; F:glycine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0003333; P:amino acid transmembrane transport; IBA:GO_Central.
DR GO; GO:0015812; P:gamma-aminobutyric acid transport; IBA:GO_Central.
DR GO; GO:0015816; P:glycine transport; IBA:GO_Central.
DR GO; GO:0098700; P:neurotransmitter loading into synaptic vesicle; IBA:GO_Central.
DR InterPro; IPR013057; AA_transpt_TM.
DR Pfam; PF01490; Aa_trans; 1.
PE 1: Evidence at protein level;
KW Cytoplasmic vesicle; Glycoprotein; Membrane; Neurotransmitter transport;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..486
FT /note="Vesicular GABA transporter"
FT /id="PRO_0000093824"
FT TOPO_DOM 1..93
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 94..114
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 115..119
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 120..140
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 141..167
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 168..188
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 189..203
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..228
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 229..249
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 250..263
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 264..284
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 285..305
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 306..326
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 327..341
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..398
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 420..421
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT TRANSMEM 422..442
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 443..457
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 458..478
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 479..486
FT /note="Lumenal, vesicle"
FT /evidence="ECO:0000255"
FT REGION 29..85
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 29..47
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 66..85
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 337
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT MUTAGEN 462
FT /note="G->R: In N2409; loss of GABA transport into synaptic
FT vesicles."
FT /evidence="ECO:0000269|PubMed:9349821"
SQ SEQUENCE 486 AA; 54439 MW; 6435C3B338552925 CRC64;
MASNRFQNLQ NWTNKHVFSN SLDYWNQELN EVPSYQNQPQ TGESGSNPPP HDRLEPIQES
VVSEQPQKDD INKQEEAKDD GHGEASEPIS ALQAAWNVTN AIQGMFIVGL PIAVKVGGWW
SIGAMVGVAY VCYWTGVLLI ECLYENGVKK RKTYREIADF YKPGFGKWVL AAQLTELLST
CIIYLVLAAD LLQSCFPSVD KAGWMMITSA SLLTCSFLDD LQIVSRLSFF NAISHLIVNL
IMVLYCLSFV SQWSFSTITF SLNINTLPTI VGMVVFGYTS HIFLPNLEGN MKNPAQFNVM
LKWSHIAAAV FKVVFGMLGF LTFGELTQEE ISNSLPNQSF KILVNLILVV KALLSYPLPF
YAAVQLLKNN LFLGYPQTPF TSCYSPDKSL REWAVTLRII LVLFTLFVAL SVPYLVELMG
LVGNITGTML SFIWPALFHL YIKEKTLNNF EKRFDQGIII MGCSVCISGV YFSSMELLRA
INSADS
