UNC4_DROME
ID UNC4_DROME Reviewed; 588 AA.
AC O77215; Q058S3;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 16-MAR-2016, sequence version 2.
DT 03-AUG-2022, entry version 153.
DE RecName: Full=Homeobox protein unc-4;
DE AltName: Full=Paired-like homeodomain protein unc-4;
DE Short=DPHD-1;
GN Name=unc-4 {ECO:0000312|EMBL:AAF48762.2}; ORFNames=CG6269;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|EMBL:AAF48762.2}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAC36333.1}
RP NUCLEOTIDE SEQUENCE [MRNA] OF 152-588, AND TISSUE SPECIFICITY.
RC STRAIN=Canton-S {ECO:0000312|EMBL:AAC36333.1};
RC TISSUE=Embryo {ECO:0000269|PubMed:9857963};
RX PubMed=9857963; DOI=10.1016/s0304-3940(98)00799-x;
RA Tabuchi K., Yoshikawa S., Yuasa Y., Sawamoto K., Okano H.;
RT "A novel Drosophila paired-like homeobox gene related to Caenorhabditis
RT elegans unc-4 is expressed in subsets of postmitotic neurons and epidermal
RT cells.";
RL Neurosci. Lett. 257:49-52(1998).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 156-588.
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Frise E., Kapadia B., Park S., Wan K.H., Yu C.,
RA Celniker S.E.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Transcription factor that regulates synaptic specificity.
CC {ECO:0000250|UniProtKB:P29506}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- TISSUE SPECIFICITY: During embryonic and third instar larval stages,
CC expressed in subsets of postmitotic neurons in the central nervous
CC system (CNS) and in the developing epidermis with a segmentally
CC repeated pattern. {ECO:0000269|PubMed:9857963}.
CC -!- SIMILARITY: Belongs to the paired homeobox family. Unc-4 subfamily.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC36333.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=ABJ17079.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AE014298; AAF48762.2; -; Genomic_DNA.
DR EMBL; AF086820; AAC36333.1; ALT_INIT; mRNA.
DR EMBL; BT029145; ABJ17079.1; ALT_INIT; mRNA.
DR RefSeq; NP_001285389.1; NM_001298460.1.
DR RefSeq; NP_573242.2; NM_133014.3.
DR AlphaFoldDB; O77215; -.
DR SMR; O77215; -.
DR BioGRID; 59079; 5.
DR IntAct; O77215; 2.
DR STRING; 7227.FBpp0074234; -.
DR PaxDb; O77215; -.
DR DNASU; 32757; -.
DR EnsemblMetazoa; FBtr0340626; FBpp0309490; FBgn0024184.
DR EnsemblMetazoa; FBtr0340627; FBpp0309491; FBgn0024184.
DR GeneID; 32757; -.
DR KEGG; dme:Dmel_CG6269; -.
DR CTD; 32757; -.
DR FlyBase; FBgn0024184; unc-4.
DR VEuPathDB; VectorBase:FBgn0024184; -.
DR eggNOG; KOG0490; Eukaryota.
DR HOGENOM; CLU_445694_0_0_1; -.
DR InParanoid; O77215; -.
DR OMA; FGKMEPM; -.
DR OrthoDB; 1061914at2759; -.
DR SignaLink; O77215; -.
DR BioGRID-ORCS; 32757; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32757; -.
DR PRO; PR:O77215; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0024184; Expressed in capitellum (Drosophila) and 5 other tissues.
DR ExpressionAtlas; O77215; baseline and differential.
DR Genevisible; O77215; DM.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IEA:InterPro.
DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR GO; GO:0010468; P:regulation of gene expression; IBA:GO_Central.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR Pfam; PF00046; Homeodomain; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW Developmental protein; Differentiation; DNA-binding; Homeobox;
KW Neurogenesis; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..588
FT /note="Homeobox protein unc-4"
FT /id="PRO_0000049348"
FT DNA_BIND 243..302
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..73
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 210..247
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 299..333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 377..500
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 522..557
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 9..73
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 483..500
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 156..160
FT /note="AAATQ -> KLSVD (in Ref. 4; ABJ17079)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 588 AA; 61123 MW; 5E125482E2E0C564 CRC64;
MVLETSEGDT KKFLTNNNNS SASRNNNSHN NNNNNHSPKE IPEETGRSSS TSSNSIPNAH
RTNAGQHLLG GSPSSACSTS VSGCGMPSEG LHPTAALQLY AAAAQLAPNG VRVPPWGPFL
QFGVPGVFGP NGPFLGRPRF DAASAGGHPN SAAAAAAATQ MAAVNASNAF ANLTGLSAAA
LRNVSAAQTT AVAAVASTVA TIQHRLMIGN RQSLPPAGPP SEGSNEDGGF PGDGDDDSSA
AKRRRSRTNF NSWQLEELER AFSASHYPDI FMREALAMRL DLKESRVAVW FQNRRAKVRK
REHTKKGPGR PAHNAQPQTC SGEPIPPNEL KAKERARRRK KLAKAIDRQA RKLQAKGITV
DLEALKAEYI SQHKANGTFS DSDLEDDGIQ IDVVGGTDSD DEGDSDAVSP VRLGGGGGGG
GGTGGGGGGG ASSSLHCGLD GDGDSSRAGS FIGGGGSLGS PSSVAPPSML SNGAVQFGKL
EPMDGNESEE RDRERDSPKP LLFPAKAFHQ LNLQSSQQHH GLVVGQGQGS GSGHGHGHQH
NHHQHHLHHG SASASAAAAV ANLVHQTSPI SMRRSNPFSI ESLLFNNT
