UNC50_MOUSE
ID UNC50_MOUSE Reviewed; 259 AA.
AC Q9CQ61;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 121.
DE RecName: Full=Protein unc-50 homolog;
DE AltName: Full=Periodontal ligament-specific protein 22;
DE Short=PDLs22;
GN Name=Unc50;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Yu L.;
RL Submitted (JAN-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Egg, Embryo, and Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP TISSUE SPECIFICITY.
RX PubMed=11302735; DOI=10.1006/bbrc.2001.4694;
RA Park J.-C., Kim Y.-B., Kim H.-J., Jang H.-S., Kim H.-S., Kim B.-O.,
RA Han K.-Y.;
RT "Isolation and characterization of cultured human periodontal ligament
RT fibroblast-specific cDNAs.";
RL Biochem. Biophys. Res. Commun. 282:1145-1153(2001).
CC -!- FUNCTION: Involved in the cell surface expression of neuronal nicotinic
CC receptors (By similarity). Binds RNA (By similarity).
CC {ECO:0000250|UniProtKB:O55227}.
CC -!- SUBCELLULAR LOCATION: Nucleus inner membrane
CC {ECO:0000250|UniProtKB:O55227}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:O55227}. Golgi apparatus membrane
CC {ECO:0000250|UniProtKB:Q53HI1}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:Q53HI1}.
CC -!- TISSUE SPECIFICITY: Highly expressed in periodontal ligament and bone
CC marrow, but not in gingival fibroblasts. {ECO:0000269|PubMed:11302735}.
CC -!- SIMILARITY: Belongs to the unc-50 family. {ECO:0000305}.
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DR EMBL; AY017214; AAK08986.1; -; mRNA.
DR EMBL; AK003273; BAB22683.1; -; mRNA.
DR EMBL; AK004433; BAB23302.1; -; mRNA.
DR EMBL; AK132757; BAE21338.1; -; mRNA.
DR EMBL; AK135885; BAE22715.1; -; mRNA.
DR EMBL; BC019484; AAH19484.1; -; mRNA.
DR CCDS; CCDS14892.1; -.
DR RefSeq; NP_080399.1; NM_026123.3.
DR RefSeq; XP_006496283.1; XM_006496220.3.
DR RefSeq; XP_006496284.1; XM_006496221.3.
DR RefSeq; XP_006496285.1; XM_006496222.3.
DR RefSeq; XP_006496286.1; XM_006496223.3.
DR AlphaFoldDB; Q9CQ61; -.
DR BioGRID; 212151; 1.
DR STRING; 10090.ENSMUSP00000027285; -.
DR PhosphoSitePlus; Q9CQ61; -.
DR MaxQB; Q9CQ61; -.
DR PaxDb; Q9CQ61; -.
DR PRIDE; Q9CQ61; -.
DR ProteomicsDB; 300198; -.
DR Antibodypedia; 17600; 66 antibodies from 21 providers.
DR DNASU; 67387; -.
DR Ensembl; ENSMUST00000027285; ENSMUSP00000027285; ENSMUSG00000026111.
DR Ensembl; ENSMUST00000114925; ENSMUSP00000110575; ENSMUSG00000026111.
DR Ensembl; ENSMUST00000118059; ENSMUSP00000113135; ENSMUSG00000026111.
DR GeneID; 67387; -.
DR KEGG; mmu:67387; -.
DR UCSC; uc007arp.1; mouse.
DR CTD; 25972; -.
DR MGI; MGI:1914637; Unc50.
DR VEuPathDB; HostDB:ENSMUSG00000026111; -.
DR eggNOG; KOG3012; Eukaryota.
DR GeneTree; ENSGT00390000018553; -.
DR InParanoid; Q9CQ61; -.
DR OMA; ISTLMWV; -.
DR OrthoDB; 1351297at2759; -.
DR PhylomeDB; Q9CQ61; -.
DR TreeFam; TF105624; -.
DR BioGRID-ORCS; 67387; 5 hits in 76 CRISPR screens.
DR ChiTaRS; Unc50; mouse.
DR PRO; PR:Q9CQ61; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q9CQ61; protein.
DR Bgee; ENSMUSG00000026111; Expressed in animal zygote and 259 other tissues.
DR ExpressionAtlas; Q9CQ61; baseline and differential.
DR Genevisible; Q9CQ61; MM.
DR GO; GO:0005794; C:Golgi apparatus; IDA:MGI.
DR GO; GO:0030173; C:integral component of Golgi membrane; IBA:GO_Central.
DR GO; GO:0005637; C:nuclear inner membrane; ISS:UniProtKB.
DR GO; GO:0003723; F:RNA binding; ISS:UniProtKB.
DR GO; GO:0034394; P:protein localization to cell surface; ISS:UniProtKB.
DR InterPro; IPR007881; UNC-50.
DR PANTHER; PTHR12841; PTHR12841; 1.
DR Pfam; PF05216; UNC-50; 1.
PE 2: Evidence at transcript level;
KW Acetylation; Golgi apparatus; Membrane; Nucleus; Phosphoprotein;
KW Reference proteome; RNA-binding; Transmembrane; Transmembrane helix.
FT CHAIN 1..259
FT /note="Protein unc-50 homolog"
FT /id="PRO_0000308962"
FT TOPO_DOM 1..82
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 83..103
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 104..112
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 113..133
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 134..163
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 164..184
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 185..187
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 188..208
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 209..222
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..259
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:Q53HI1"
FT MOD_RES 6
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q53HI1"
SQ SEQUENCE 259 AA; 30429 MW; C11FD3CD95FBD4A6 CRC64;
MLPSTSLSSS MHGNGVLNSR DAARHTAGAK RYKYLRRLFR FRQMDFEFAA WQMLYLFTSP
QRVYRNFHYR KQTKDQWARD DPAFLVLLSI WLCVSTIGFG FVLDMGFFET IKLLLWVVFI
DCVGVGLLIS TLMWFVSNKY LVKRQSRDYD VEWGYAFDVH LNAFYPLLVI LHFIQLFFIN
HVILTDTFIG YLVGNTLWLI AVGYYIYVTF LGYSALPFLK NTVILLYPFA PLMVLYGLSL
ALGWNFTHTL CSFYKYRVK
