UNC55_CAEEL
ID UNC55_CAEEL Reviewed; 370 AA.
AC G5ECR9; G5EDI5;
DT 16-JAN-2019, integrated into UniProtKB/Swiss-Prot.
DT 14-DEC-2011, sequence version 1.
DT 03-AUG-2022, entry version 91.
DE RecName: Full=Nuclear hormone receptor unc-55 {ECO:0000305};
DE AltName: Full=Uncoordinated protein 55 {ECO:0000303|PubMed:18652814};
GN Name=unc-55 {ECO:0000312|WormBase:F55D12.4a};
GN ORFNames=F55D12.4 {ECO:0000312|WormBase:F55D12.4a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239 {ECO:0000312|EMBL:ABQ96204.1};
RN [1] {ECO:0000312|EMBL:ABQ96204.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM A), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RX PubMed=9852581; DOI=10.1523/jneurosci.18-24-10438.1998;
RA Zhou H.M., Walthall W.W.;
RT "UNC-55, an orphan nuclear hormone receptor, orchestrates synaptic
RT specificity among two classes of motor neurons in Caenorhabditis elegans.";
RL J. Neurosci. 18:10438-10444(1998).
RN [2] {ECO:0000312|EMBL:ABQ96199.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM B), FUNCTION, ALTERNATIVE SPLICING, AND
RP TISSUE SPECIFICITY.
RX PubMed=18652814; DOI=10.1016/j.ydbio.2008.06.034;
RA Shan G., Walthall W.W.;
RT "Copulation in C. elegans males requires a nuclear hormone receptor.";
RL Dev. Biol. 322:11-20(2008).
RN [3] {ECO:0000312|Proteomes:UP000001940}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2 {ECO:0000312|Proteomes:UP000001940};
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [4] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=7869081; DOI=10.1523/jneurosci.15-02-01035.1995;
RA Walthall W.W., Plunkett J.A.;
RT "Genetic transformation of the synaptic pattern of a motoneuron class in
RT Caenorhabditis elegans.";
RL J. Neurosci. 15:1035-1043(1995).
RN [5] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=15882588; DOI=10.1016/j.ydbio.2005.01.032;
RA Shan G., Kim K., Li C., Walthall W.W.;
RT "Convergent genetic programs regulate similarities and differences between
RT related motor neuron classes in Caenorhabditis elegans.";
RL Dev. Biol. 280:494-503(2005).
RN [6] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=29033363; DOI=10.1016/j.devcel.2017.09.013;
RA Yu B., Wang X., Wei S., Fu T., Dzakah E.E., Waqas A., Walthall W.W.,
RA Shan G.;
RT "Convergent Transcriptional Programs Regulate cAMP Levels in C. elegans
RT GABAergic Motor Neurons.";
RL Dev. Cell 43:212-226(2017).
RN [7]
RP FUNCTION, AND MUTAGENESIS OF 195-GLN--ASN-370.
RX PubMed=28056346; DOI=10.1016/j.neuron.2016.11.036;
RA Kerk S.Y., Kratsios P., Hart M., Mourao R., Hobert O.;
RT "Diversification of C. elegans Motor Neuron Identity via Selective Effector
RT Gene Repression.";
RL Neuron 93:80-98(2017).
CC -!- FUNCTION: Transcription factor (PubMed:28056346, PubMed:29033363).
CC Involved in motor neuron fate determination and maintenance, acting as
CC a transcriptional repressor to counteract gene activation by
CC transcription factor unc-3 in a subset of motor neurons
CC (PubMed:28056346). Probably acts by binding to specific promoter
CC elements (PubMed:28056346). Required for ventral D (VD) motor neurons
CC to adopt their normal synaptic pattern (PubMed:7869081). Suppresses
CC expression of flp-13 in VD motor neurons to ensure formation of the
CC correct synaptic pattern (PubMed:15882588). Maintains low cAMP levels
CC in VD motor neurons by enhancing expression of pde-4 which hydrolyzes
CC cAMP and repressing expression of acy-1 which catalyzes cAMP formation
CC (PubMed:29033363). This prevents respecification of synapses by VD
CC neurons (PubMed:29033363). During copulation, required in males for
CC correct movement of the spicules, a pair of prong-like structures which
CC are inserted into the vulva of the hermaphrodite and anchor the male to
CC the hermaphrodite (PubMed:18652814). Required for spicule prodding
CC which allows detection of the vulva location and for spicule insertion
CC into the vulva (PubMed:18652814). {ECO:0000269|PubMed:15882588,
CC ECO:0000269|PubMed:18652814, ECO:0000269|PubMed:28056346,
CC ECO:0000269|PubMed:29033363, ECO:0000269|PubMed:7869081}.
CC -!- FUNCTION: [Isoform a]: Required for normal locomotion.
CC {ECO:0000269|PubMed:18652814}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255,
CC ECO:0000255|RuleBase:RU004334}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:F55D12.4a};
CC IsoId=G5ECR9-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:F55D12.4b};
CC IsoId=G5ECR9-2; Sequence=VSP_060026;
CC -!- TISSUE SPECIFICITY: Expressed in spicule protractor and retractor
CC muscles, and the male-specific neuron CP9 (PubMed:18652814). Expressed
CC in the nerve ring, and preanal ganglion (PubMed:9852581). Expressed in
CC asymmetric motor neurons (PubMed:9852581, PubMed:29033363). Expressed
CC in ventral D motor neurons (PubMed:9852581, PubMed:7869081,
CC PubMed:15882588, PubMed:29033363). Isoform a: Expressed in males and
CC hermaphrodites (PubMed:18652814). Isoform b: Only expressed in males
CC (PubMed:18652814). {ECO:0000269|PubMed:15882588,
CC ECO:0000269|PubMed:18652814, ECO:0000269|PubMed:29033363,
CC ECO:0000269|PubMed:7869081, ECO:0000269|PubMed:9852581}.
CC -!- DEVELOPMENTAL STAGE: Expression in the asymmetric and ventral D motor
CC neurons in the ventral nerve cord is restricted to the postembryonic
CC stages L2 and L3. {ECO:0000269|PubMed:9852581}.
CC -!- SIMILARITY: Belongs to the nuclear hormone receptor family.
CC {ECO:0000255, ECO:0000255|RuleBase:RU004334}.
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DR EMBL; EF601082; ABQ96204.1; -; mRNA.
DR EMBL; EF601077; ABQ96199.1; -; mRNA.
DR EMBL; BX284601; CAP16277.2; -; Genomic_DNA.
DR EMBL; BX284601; CAP16278.2; -; Genomic_DNA.
DR RefSeq; NP_001122482.2; NM_001129010.2. [G5ECR9-1]
DR RefSeq; NP_001122483.2; NM_001129011.2. [G5ECR9-2]
DR AlphaFoldDB; G5ECR9; -.
DR SMR; G5ECR9; -.
DR STRING; 6239.F55D12.4a; -.
DR PaxDb; G5ECR9; -.
DR PeptideAtlas; G5ECR9; -.
DR EnsemblMetazoa; F55D12.4a.1; F55D12.4a.1; WBGene00006790. [G5ECR9-1]
DR EnsemblMetazoa; F55D12.4b.1; F55D12.4b.1; WBGene00006790. [G5ECR9-2]
DR GeneID; 192082; -.
DR KEGG; cel:CELE_F55D12.4; -.
DR CTD; 192082; -.
DR WormBase; F55D12.4a; CE43454; WBGene00006790; unc-55. [G5ECR9-1]
DR WormBase; F55D12.4b; CE43459; WBGene00006790; unc-55. [G5ECR9-2]
DR eggNOG; KOG3575; Eukaryota.
DR GeneTree; ENSGT00970000196030; -.
DR HOGENOM; CLU_007368_20_1_1; -.
DR InParanoid; G5ECR9; -.
DR OMA; NCAIDVQ; -.
DR OrthoDB; 1413421at2759; -.
DR PhylomeDB; G5ECR9; -.
DR PRO; PR:G5ECR9; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00006790; Expressed in pharyngeal muscle cell (C elegans) and 2 other tissues.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0004879; F:nuclear receptor activity; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR Gene3D; 1.10.565.10; -; 1.
DR Gene3D; 3.30.50.10; -; 1.
DR InterPro; IPR035500; NHR-like_dom_sf.
DR InterPro; IPR000536; Nucl_hrmn_rcpt_lig-bd.
DR InterPro; IPR001723; Nuclear_hrmn_rcpt.
DR InterPro; IPR001628; Znf_hrmn_rcpt.
DR InterPro; IPR013088; Znf_NHR/GATA.
DR Pfam; PF00104; Hormone_recep; 1.
DR Pfam; PF00105; zf-C4; 1.
DR PRINTS; PR00398; STRDHORMONER.
DR PRINTS; PR00047; STROIDFINGER.
DR SMART; SM00430; HOLI; 1.
DR SMART; SM00399; ZnF_C4; 1.
DR SUPFAM; SSF48508; SSF48508; 1.
DR PROSITE; PS51843; NR_LBD; 1.
DR PROSITE; PS00031; NUCLEAR_REC_DBD_1; 1.
DR PROSITE; PS51030; NUCLEAR_REC_DBD_2; 1.
PE 1: Evidence at protein level;
KW Activator; Alternative splicing; DNA-binding; Metal-binding; Nucleus;
KW Receptor; Reference proteome; Repressor; Transcription;
KW Transcription regulation; Zinc; Zinc-finger.
FT CHAIN 1..370
FT /note="Nuclear hormone receptor unc-55"
FT /id="PRO_0000446101"
FT DOMAIN 95..355
FT /note="NR LBD"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT DNA_BIND 18..93
FT /note="Nuclear receptor"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 21..41
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT ZN_FING 57..76
FT /note="NR C4-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00407"
FT REGION 216..240
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 344..355
FT /note="AF-2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01189"
FT COMPBIAS 224..240
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 90..102
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_060026"
FT MUTAGEN 195..370
FT /note="Missing: In ot718; uncoordinated locomotory
FT phenotype. Induces ectopic expression of TGF-b family
FT members unc-129 and dbl-1, and of potassium channel slo-2,
FT in AS motor neurons. Ectopic expression is repressed in an
FT unc-3 mutant background."
FT /evidence="ECO:0000269|PubMed:28056346"
SQ SEQUENCE 370 AA; 41505 MW; 3CACA8E42B8D3D3B CRC64;
MQDGSSGAAS LGNSSPDATD CVVCGDKSSG KHYGQFSCEG CKSFFKRSIR RSLSYTCRAT
KNCAIDVQHR NQCQYCRLTK CIRMGMRKEG RRSNETQTAV SAVQRGRLPV TMPSLFPPNM
FLRSPFPFMS VPFNPLMTAQ FTKPSIKESI FEFAAQTIFA TVNWARTSMS NLVKGDQLIL
LRHSWTPIFI FALAQSNFAL NLSTHLTAVT ATAASTENGS SSLGSKSEDE EKSEEKPERV
FDEPQFQGFQ AKIDKIRDFH LDVVESSSLR AVLLFSCDEE ALEEKGKIEE IVEKLKSAVD
EYCKMNKRSE RYHQICECLQ LLKSTRNLPI SRLFFSRLLG TTPLETILSD LLITPPPPTL
PFFPQLPSRN
