UNC5_DROME
ID UNC5_DROME Reviewed; 1072 AA.
AC Q95TU8; Q9NBL0; Q9V7B5;
DT 11-OCT-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Netrin receptor unc-5;
DE AltName: Full=Unc5 netrin receptor;
DE Flags: Precursor;
GN Name=unc-5; ORFNames=CG8166;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=11719202; DOI=10.1016/s0896-6273(01)00505-0;
RA Keleman K., Dickson B.J.;
RT "Short- and long-range repulsion by the Drosophila Unc5 netrin receptor.";
RL Neuron 32:605-617(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP FUNCTION.
RX PubMed=12765609; DOI=10.1016/s0896-6273(03)00289-7;
RA Freeman M.R., Delrow J., Kim J., Johnson E., Doe C.Q.;
RT "Unwrapping glial biology: Gcm target genes regulating glial development,
RT diversification, and function.";
RL Neuron 38:567-580(2003).
CC -!- FUNCTION: Receptor for netrin required for motor axon guidance.
CC Mediates both short- and long-range axon motor repulsion in the
CC developing nervous system upon ligand binding. Also involved in glial
CC migration. While short-range repulsion requires both fra and unc-5,
CC long-range repulsion only requires unc-5. {ECO:0000269|PubMed:11719202,
CC ECO:0000269|PubMed:12765609}.
CC -!- INTERACTION:
CC Q95TU8; Q0E9F2: sns; NbExp=3; IntAct=EBI-3411972, EBI-6895883;
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Prior to gastrulation, it is strongly expressed in
CC the presumptive mesoderm. Mesodermal expression begins to fade during
CC stages 13-14, persisting only in the cells that form the dorsal vessel.
CC Expressed within the CNS from late stage 13, shortly after the first
CC axons have extended. Detected in several dispersed clusters of cells
CC within the CNS, increasing in number as development proceeds. Also
CC expressed in the peripheral and exit glia, which migrate laterally out
CC of the CNS between stages 14 and 17. Strongly expressed in motor axons
CC that exit the CNS ipsilaterally via the segmental nerve root (SN). Not
CC expressed on either commissural or longitudinal axons within the CNS,
CC nor on motor axons that exit via the intersegmental nerve (ISN). In the
CC periphery, it is detected on all branches of the SN. Also expressed at
CC high level in exit and peripheral glia along both the SN and ISN.
CC {ECO:0000269|PubMed:11719202}.
CC -!- PTM: Phosphorylated on different cytoplasmic tyrosine residues.
CC {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the unc-5 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF247762; AAF74193.1; -; mRNA.
DR EMBL; AE013599; AAF58143.2; -; Genomic_DNA.
DR EMBL; AY058501; AAL13730.1; -; mRNA.
DR RefSeq; NP_001286450.1; NM_001299521.1.
DR RefSeq; NP_611033.2; NM_137189.3.
DR AlphaFoldDB; Q95TU8; -.
DR SMR; Q95TU8; -.
DR BioGRID; 62440; 8.
DR IntAct; Q95TU8; 3.
DR STRING; 7227.FBpp0086514; -.
DR GlyGen; Q95TU8; 2 sites.
DR PaxDb; Q95TU8; -.
DR PRIDE; Q95TU8; -.
DR DNASU; 36703; -.
DR EnsemblMetazoa; FBtr0087382; FBpp0086514; FBgn0034013.
DR EnsemblMetazoa; FBtr0339998; FBpp0309017; FBgn0034013.
DR GeneID; 36703; -.
DR KEGG; dme:Dmel_CG8166; -.
DR UCSC; CG8166-RA; d. melanogaster.
DR CTD; 36703; -.
DR FlyBase; FBgn0034013; unc-5.
DR VEuPathDB; VectorBase:FBgn0034013; -.
DR eggNOG; KOG1480; Eukaryota.
DR GeneTree; ENSGT00950000182815; -.
DR HOGENOM; CLU_014383_0_0_1; -.
DR InParanoid; Q95TU8; -.
DR OMA; TIEHNLI; -.
DR OrthoDB; 334938at2759; -.
DR PhylomeDB; Q95TU8; -.
DR Reactome; R-DME-373752; Netrin-1 signaling.
DR BioGRID-ORCS; 36703; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 36703; -.
DR PRO; PR:Q95TU8; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034013; Expressed in wing disc and 29 other tissues.
DR ExpressionAtlas; Q95TU8; baseline and differential.
DR Genevisible; Q95TU8; DM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005043; F:netrin receptor activity involved in chemorepulsion; ISS:FlyBase.
DR GO; GO:0007411; P:axon guidance; IMP:FlyBase.
DR GO; GO:0008347; P:glial cell migration; IMP:FlyBase.
DR GO; GO:0008045; P:motor neuron axon guidance; IMP:FlyBase.
DR GO; GO:0030335; P:positive regulation of cell migration; IMP:FlyBase.
DR GO; GO:0007432; P:salivary gland boundary specification; IMP:FlyBase.
DR Gene3D; 1.10.533.10; -; 1.
DR Gene3D; 2.20.100.10; -; 2.
DR Gene3D; 2.60.40.10; -; 2.
DR InterPro; IPR011029; DEATH-like_dom_sf.
DR InterPro; IPR000488; Death_domain.
DR InterPro; IPR007110; Ig-like_dom.
DR InterPro; IPR036179; Ig-like_dom_sf.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR013098; Ig_I-set.
DR InterPro; IPR003599; Ig_sub.
DR InterPro; IPR003598; Ig_sub2.
DR InterPro; IPR000884; TSP1_rpt.
DR InterPro; IPR036383; TSP1_rpt_sf.
DR InterPro; IPR037936; UNC5.
DR InterPro; IPR033772; UPA.
DR InterPro; IPR000906; ZU5_dom.
DR PANTHER; PTHR12582; PTHR12582; 1.
DR Pfam; PF00531; Death; 1.
DR Pfam; PF07679; I-set; 1.
DR Pfam; PF00090; TSP_1; 2.
DR Pfam; PF17217; UPA; 1.
DR Pfam; PF00791; ZU5; 1.
DR SMART; SM00005; DEATH; 1.
DR SMART; SM00409; IG; 2.
DR SMART; SM00408; IGc2; 1.
DR SMART; SM00209; TSP1; 2.
DR SMART; SM00218; ZU5; 1.
DR SUPFAM; SSF47986; SSF47986; 1.
DR SUPFAM; SSF48726; SSF48726; 1.
DR SUPFAM; SSF82895; SSF82895; 2.
DR PROSITE; PS50017; DEATH_DOMAIN; 1.
DR PROSITE; PS50835; IG_LIKE; 1.
DR PROSITE; PS50092; TSP1; 2.
DR PROSITE; PS51145; ZU5; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Disulfide bond; Glycoprotein; Immunoglobulin domain;
KW Membrane; Phosphoprotein; Receptor; Reference proteome; Repeat; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..1072
FT /note="Netrin receptor unc-5"
FT /id="PRO_0000036082"
FT TOPO_DOM 31..440
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 441..461
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 462..1072
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 128..224
FT /note="Ig-like"
FT DOMAIN 232..314
FT /note="Ig-like C2-type"
FT DOMAIN 324..379
FT /note="TSP type-1 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT DOMAIN 398..499
FT /note="TSP type-1 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00210"
FT DOMAIN 654..802
FT /note="ZU5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00485"
FT DOMAIN 980..1067
FT /note="Death"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00064"
FT REGION 77..100
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 79
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 300
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 149..207
FT /evidence="ECO:0000250"
FT DISULFID 253..303
FT /evidence="ECO:0000250"
FT DISULFID 336..375
FT /evidence="ECO:0000250"
FT DISULFID 338..378
FT /evidence="ECO:0000250"
FT DISULFID 352..364
FT /evidence="ECO:0000250"
FT CONFLICT 885
FT /note="P -> S (in Ref. 1; AAF74193)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1072 AA; 116418 MW; A0A6B5A96B10138F CRC64;
MAVINKAGNV IALLLVKLQL ILLFTLSVSG ELPQLDYGSL SASLEEDAID PLTAMAPFAN
SLVTEESAQH KNNAELLGNS SEDENVRPQQ GSSSSGLGSS GAAGGSGILL EEFNSGKLSP
GEASNTLPIF LIEPESVFVV KNRPAVLKCK ASHSLQVIFK CSGSSQPPPS THETHVDPHT
GVNMEEVTAT IHRDLVDEFF GDGPFKCECH AWSSRGVVKS QAATVHIAYI RKSFNQSPTS
LRLELGSRAE LRCEPPGGFP EPKLTWHKNN AVITADSEPG ITVSAGTLIF RQVALQHMAN
YSCSAENIAG RRVSDSAVLI VYVNGGWSTW SPWRECKCAG KPSQGRKRSR TCNNPMPLNG
GAQCPGPQIQ KSADCAACPE DTQIVSPDGF DISSSKRMAR WSAWSDWSIC SAECIQVRRR
KCLTQGQTQI SSEAEEAGDL LLGAPGVGMA ALIAAAGVGA VGSPSEATGS SSDIIPGYGK
SLCAGKDIQT AECRGEQCQI GKDDFDWTLY LGLAFITAVC FAFGTALICC ARRGIRTNPH
YNMARSVMDA DYMPGVVEKK EMRMHIEASN MGYDYVNPGH RYLPGEHIMG MGIGCGGVTE
HHYDVPNLSA NYTNPIDHLS VDYLSETGES STADTSNSTF DMNGKLSILN ASKSSTYEML
GSAGGQLRLY GGELLLFVPE HAIGKHVKKH VSLLLLSDEC SRVSCATESS ILCSSVVHSA
PRNYSFVKPV ILKIPHCLVA PEQWHVHIYH ADSEHDELSV NWRRAVSVGE ETINTPMFVQ
LEATHVFIMT EQLGHFTVVA EPRIQQPSIK MKLLAFSQHT PPSNANCSLR IYVVKDFPNS
RDICANVEAK LGGSFLGESQ VFAFTLNSRN LNIRVRSADV EAAAPYEHAI PYQHILSNNS
ILHCEFSLRR QDQNSLCVDF GQGSEDDYYT FNIPAHSMSG SAEELASTTN TTISIDRQGN
YVNESCVMDF VQLPHATKRL ICGALDPPRA DERDWRLLAK KLNTDRYIAY FATKASPTEQ
ILNLWECRAN SSPGSSSNSV SHTIMALLLT LKEMGRQDVL DIIVETIGPL WI
