UNC62_CAEEL
ID UNC62_CAEEL Reviewed; 564 AA.
AC Q9N5D6; Q5TKQ8; Q8MXU6; Q8MXU7; Q8WRQ5; Q8WRQ6; Q9N5D5;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 156.
DE RecName: Full=Homeobox protein unc-62;
DE AltName: Full=Uncoordinated protein 62;
GN Name=unc-62; ORFNames=T28F12.2;
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAL65143.1}
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS A; B; E AND F), FUNCTION,
RP DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC STRAIN=Bristol N2 {ECO:0000312|EMBL:AAL65143.1};
RX PubMed=12399316; DOI=10.1242/dev.129.22.5255;
RA Van Auken K., Weaver D., Robertson B., Sundaram M., Saldi T., Edgar L.,
RA Elling U., Lee M., Boese Q., Wood W.B.;
RT "Roles of the Homothorax/Meis/Prep homolog UNC-62 and the Exd/Pbx homologs
RT CEH-20 and CEH-40 in C. elegans embryogenesis.";
RL Development 129:5255-5268(2002).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3] {ECO:0000305}
RP FUNCTION, AND DEVELOPMENTAL STAGE.
RX PubMed=15750187; DOI=10.1242/dev.01569;
RA Yang L., Sym M., Kenyon C.;
RT "The roles of two C. elegans HOX co-factor orthologs in cell migration and
RT vulva development.";
RL Development 132:1413-1428(2005).
RN [4]
RP FUNCTION, INTERACTION WITH CEH-60, AND DISRUPTION PHENOTYPE.
RX PubMed=30956009; DOI=10.1016/j.devcel.2019.03.002;
RA Dowen R.H.;
RT "CEH-60/PBX and UNC-62/MEIS Coordinate a Metabolic Switch that Supports
RT Reproduction in C. elegans.";
RL Dev. Cell 49:235-250.e7(2019).
RN [5]
RP FUNCTION, AND INTERACTION WITH CEH-60.
RX PubMed=31675356; DOI=10.1371/journal.pbio.3000499;
RA Van de Walle P., Geens E., Baggerman G., Jose Naranjo-Galindo F.,
RA Askjaer P., Schoofs L., Temmerman L.;
RT "CEH-60/PBX regulates vitellogenesis and cuticle permeability through
RT intestinal interaction with UNC-62/MEIS in Caenorhabditis elegans.";
RL PLoS Biol. 17:e3000499-e3000499(2019).
CC -!- FUNCTION: Acts redundantly with ceh-20 and ceh-40 to perform
CC overlapping roles during embryogenesis (PubMed:12399316). Required for
CC postembryonic development of the ectoderm, including the Q, V and P
CC cell lineages, playing a crucial role in ensuring that these cells and
CC their descendants undergo their invariant patterns of cell division,
CC migration, fusion and morphogenesis (PubMed:15750187). Has a role in
CC the mig-13 pathway to promote anterior migration of neuroblasts in the
CC Q lineage (PubMed:15750187). Required for multiple roles in regulating
CC vulva development (PubMed:15750187). Associates with homeobox ceh-60 to
CC regulate gene expression, including repression of genes involved in
CC innate immunity and activation of genes involved in vitellogenesis
CC (PubMed:30956009, PubMed:31675356). Involved in lipid homeostasis,
CC contributing to the formation of the cuticle (PubMed:30956009,
CC PubMed:31675356). {ECO:0000269|PubMed:12399316,
CC ECO:0000269|PubMed:15750187, ECO:0000269|PubMed:30956009,
CC ECO:0000269|PubMed:31675356}.
CC -!- SUBUNIT: Forms a heterodimer with homeobox ceh-60.
CC {ECO:0000269|PubMed:30956009, ECO:0000269|PubMed:31675356}.
CC -!- INTERACTION:
CC Q9N5D6; P41779: ceh-20; NbExp=3; IntAct=EBI-319775, EBI-2416925;
CC Q9N5D6; Q19503: ceh-40; NbExp=4; IntAct=EBI-319775, EBI-319764;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=7;
CC Name=a {ECO:0000269|PubMed:12399316}; Synonyms=1a-7b
CC {ECO:0000269|PubMed:12399316};
CC IsoId=Q9N5D6-1; Sequence=Displayed;
CC Name=b {ECO:0000269|PubMed:12399316}; Synonyms=1b-7b
CC {ECO:0000269|PubMed:12399316};
CC IsoId=Q9N5D6-2; Sequence=VSP_052829;
CC Name=c {ECO:0000269|PubMed:9851916};
CC IsoId=Q9N5D6-3; Sequence=VSP_052830, VSP_052831;
CC Name=d {ECO:0000269|PubMed:9851916};
CC IsoId=Q9N5D6-4; Sequence=VSP_052827;
CC Name=e {ECO:0000269|PubMed:12399316}; Synonyms=1b-7a
CC {ECO:0000269|PubMed:12399316};
CC IsoId=Q9N5D6-5; Sequence=VSP_052829, VSP_052832;
CC Name=f {ECO:0000269|PubMed:12399316}; Synonyms=1a-7a
CC {ECO:0000269|PubMed:12399316};
CC IsoId=Q9N5D6-6; Sequence=VSP_052832;
CC Name=g {ECO:0000269|PubMed:9851916};
CC IsoId=Q9N5D6-7; Sequence=VSP_052828;
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically. Isoform
CC a and isoform f are more abundant than isoform b and isoform e, but
CC their temporal patterns of accumulation are similar. All are present in
CC adult germline, pregastrulation embryos, later embryos and adult soma.
CC {ECO:0000269|PubMed:12399316, ECO:0000269|PubMed:15750187}.
CC -!- DISRUPTION PHENOTYPE: Worms exhibit severe posterior disorganization
CC during embryogenesis (Nob phenotype) (PubMed:12399316). Mutants exhibit
CC Q cell migration defects, affecting cell position along the anterior
CC posterior axis (PubMed:12399316). RNAi mediated knockdown abolishes
CC nuclear localization of ceh-60 (PubMed:30956009). RNAi mediated
CC knockdown targeted to the intestine represses expression of
CC vitellogenin genes; further repressed on ceh-60 mutant background
CC (PubMed:30956009). {ECO:0000269|PubMed:12399316,
CC ECO:0000269|PubMed:30956009}.
CC -!- SIMILARITY: Belongs to the TALE/MEIS homeobox family. {ECO:0000255}.
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DR EMBL; AF427474; AAL65142.1; -; mRNA.
DR EMBL; AF427475; AAL65143.1; -; mRNA.
DR EMBL; AF427476; AAL65144.1; -; mRNA.
DR EMBL; AF427477; AAL65145.1; -; mRNA.
DR EMBL; FO081534; CCD72261.1; -; Genomic_DNA.
DR EMBL; FO081534; CCD72262.1; -; Genomic_DNA.
DR EMBL; FO081534; CCD72263.1; -; Genomic_DNA.
DR EMBL; FO081534; CCD72264.1; -; Genomic_DNA.
DR EMBL; FO081534; CCD72265.1; -; Genomic_DNA.
DR EMBL; FO081534; CCD72266.1; -; Genomic_DNA.
DR EMBL; FO081534; CCD72267.1; -; Genomic_DNA.
DR RefSeq; NP_001024169.1; NM_001028998.2. [Q9N5D6-1]
DR RefSeq; NP_001024170.1; NM_001028999.2. [Q9N5D6-2]
DR RefSeq; NP_001024171.1; NM_001029000.1.
DR RefSeq; NP_001024172.1; NM_001029001.2.
DR RefSeq; NP_001024173.1; NM_001029002.2. [Q9N5D6-5]
DR RefSeq; NP_001024174.1; NM_001029003.2.
DR RefSeq; NP_001024175.1; NM_001029004.3. [Q9N5D6-7]
DR AlphaFoldDB; Q9N5D6; -.
DR SMR; Q9N5D6; -.
DR BioGRID; 43897; 94.
DR DIP; DIP-26061N; -.
DR IntAct; Q9N5D6; 79.
DR STRING; 6239.T28F12.2a; -.
DR EPD; Q9N5D6; -.
DR PaxDb; Q9N5D6; -.
DR PeptideAtlas; Q9N5D6; -.
DR EnsemblMetazoa; T28F12.2a.1; T28F12.2a.1; WBGene00006796. [Q9N5D6-1]
DR EnsemblMetazoa; T28F12.2b.1; T28F12.2b.1; WBGene00006796. [Q9N5D6-2]
DR EnsemblMetazoa; T28F12.2c.1; T28F12.2c.1; WBGene00006796. [Q9N5D6-3]
DR EnsemblMetazoa; T28F12.2c.2; T28F12.2c.2; WBGene00006796. [Q9N5D6-3]
DR EnsemblMetazoa; T28F12.2d.1; T28F12.2d.1; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2d.10; T28F12.2d.10; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2d.2; T28F12.2d.2; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2d.3; T28F12.2d.3; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2d.4; T28F12.2d.4; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2d.5; T28F12.2d.5; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2d.6; T28F12.2d.6; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2d.7; T28F12.2d.7; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2d.8; T28F12.2d.8; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2d.9; T28F12.2d.9; WBGene00006796. [Q9N5D6-4]
DR EnsemblMetazoa; T28F12.2e.1; T28F12.2e.1; WBGene00006796. [Q9N5D6-5]
DR EnsemblMetazoa; T28F12.2f.1; T28F12.2f.1; WBGene00006796. [Q9N5D6-6]
DR EnsemblMetazoa; T28F12.2g.1; T28F12.2g.1; WBGene00006796. [Q9N5D6-7]
DR GeneID; 178845; -.
DR UCSC; T28F12.2a.1; c. elegans. [Q9N5D6-1]
DR CTD; 178845; -.
DR WormBase; T28F12.2a; CE21219; WBGene00006796; unc-62. [Q9N5D6-1]
DR WormBase; T28F12.2b; CE21220; WBGene00006796; unc-62. [Q9N5D6-2]
DR WormBase; T28F12.2c; CE31073; WBGene00006796; unc-62. [Q9N5D6-3]
DR WormBase; T28F12.2d; CE31074; WBGene00006796; unc-62. [Q9N5D6-4]
DR WormBase; T28F12.2e; CE33584; WBGene00006796; unc-62. [Q9N5D6-5]
DR WormBase; T28F12.2f; CE33585; WBGene00006796; unc-62. [Q9N5D6-6]
DR WormBase; T28F12.2g; CE37795; WBGene00006796; unc-62. [Q9N5D6-7]
DR eggNOG; KOG0773; Eukaryota.
DR GeneTree; ENSGT00940000168996; -.
DR InParanoid; Q9N5D6; -.
DR OMA; PENNRAM; -.
DR OrthoDB; 1126341at2759; -.
DR PhylomeDB; Q9N5D6; -.
DR SignaLink; Q9N5D6; -.
DR PRO; PR:Q9N5D6; -.
DR Proteomes; UP000001940; Chromosome V.
DR Bgee; WBGene00006796; Expressed in embryo and 3 other tissues.
DR ExpressionAtlas; Q9N5D6; baseline and differential.
DR GO; GO:0000785; C:chromatin; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:WormBase.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IMP:UniProtKB.
DR GO; GO:0055088; P:lipid homeostasis; IMP:UniProtKB.
DR GO; GO:0007501; P:mesodermal cell fate specification; IMP:WormBase.
DR GO; GO:0042692; P:muscle cell differentiation; IMP:WormBase.
DR GO; GO:0045727; P:positive regulation of translation; IMP:WormBase.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR008422; Homeobox_KN_domain.
DR InterPro; IPR032453; PKNOX/Meis_N.
DR Pfam; PF05920; Homeobox_KN; 1.
DR Pfam; PF16493; Meis_PKNOX_N; 1.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Developmental protein; DNA-binding; Homeobox;
KW Nucleus; Reference proteome.
FT CHAIN 1..564
FT /note="Homeobox protein unc-62"
FT /id="PRO_0000341483"
FT DOMAIN 133..218
FT /note="MEIS N-terminal"
FT /evidence="ECO:0000255"
FT DNA_BIND 392..454
FT /note="Homeobox; TALE-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 40..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 216..270
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 293..313
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 328..397
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 455..503
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 219..243
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 328..362
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 375..393
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 457..471
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 481..503
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..453
FT /note="Missing (in isoform d)"
FT /evidence="ECO:0000303|PubMed:9851916"
FT /id="VSP_052827"
FT VAR_SEQ 1..74
FT /note="Missing (in isoform g)"
FT /evidence="ECO:0000303|PubMed:9851916"
FT /id="VSP_052828"
FT VAR_SEQ 2..41
FT /note="SGDSKVWAHASADTWAVQNGISTYDLDTSSIKREKRDHNE -> AQR (in
FT isoform b and isoform e)"
FT /evidence="ECO:0000303|PubMed:12399316"
FT /id="VSP_052829"
FT VAR_SEQ 2..5
FT /note="SGDS -> FNFQ (in isoform c)"
FT /evidence="ECO:0000303|PubMed:9851916"
FT /id="VSP_052830"
FT VAR_SEQ 6..416
FT /note="Missing (in isoform c)"
FT /evidence="ECO:0000303|PubMed:9851916"
FT /id="VSP_052831"
FT VAR_SEQ 364..413
FT /note="GDEFSVCGSNDDGRDSVLSDSANGSQNGKRKVPKVFSKEAITKFRAWLFH
FT -> APSSASSSSSSKNKPSTPNGGRIGKSRGRGIFPKQATNRLRQWLFQ (in
FT isoform e and isoform f)"
FT /evidence="ECO:0000303|PubMed:12399316"
FT /id="VSP_052832"
SQ SEQUENCE 564 AA; 60889 MW; 1CA94964980BC2C5 CRC64;
MSGDSKVWAH ASADTWAVQN GISTYDLDTS SIKREKRDHN EQFNDGYGPP PGSASADPAS
YIADPAAFYN LYTNMGGAPT STPMMHHEMG EAMKRDKESI YAHPLYPLLV LLFEKCELAT
STPRDTSRDG STSSDVCSSA SFKDDLNEFV RHTQENADKQ YYVPNPQLDQ IMLQSIQMLR
FHLLELEKVH ELCDNFCNRY VVCLKGKMPL DIVGDERASS SQPPMSPGSM GHLGHSSSPS
MAGGATPMHY PPPYEPQSVP LPENAGVMGG HPMEGSSMAY SMAGMAAAAA SSSSSSNQAG
DHPLANGGTL HSTAGASQTL LPIAVSSPST CSSGGLRQDS TPLSGETPMG HANGNSMDSI
SEAGDEFSVC GSNDDGRDSV LSDSANGSQN GKRKVPKVFS KEAITKFRAW LFHNLTHPYP
SEEQKKQLAK ETGLTILQVN NWFINARRRI VQPMIDQNNR AGRSGQMNVC KNRRRNRSEQ
SPGPSPDSGS DSGANYSPDP SSLAASTAMP YPAEFYMQRT MPYGGFPSFT NPAMPFMNPM
MGFQVAPTVD ALSQQWVDLS APHE
