UNC7_CAEEL
ID UNC7_CAEEL Reviewed; 522 AA.
AC Q03412;
DT 01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1993, sequence version 1.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Innexin unc-7;
DE AltName: Full=Uncoordinated protein 7;
GN Name=unc-7 {ECO:0000312|WormBase:R07D5.1a};
GN Synonyms=unc-12 {ECO:0000312|WormBase:R07D5.1a},
GN unc-124 {ECO:0000312|WormBase:R07D5.1a};
GN ORFNames=R07D5.1 {ECO:0000312|WormBase:R07D5.1a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=Bristol N2;
RX PubMed=7681023; DOI=10.1093/genetics/133.3.527;
RA Starich T.A., Herman R.K., Shaw J.E.;
RT "Molecular and genetic analysis of unc-7, a Caenorhabditis elegans gene
RT required for coordinated locomotion.";
RL Genetics 133:527-541(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [3]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=17891142; DOI=10.1038/nn1981;
RA Van Buskirk C., Sternberg P.W.;
RT "Epidermal growth factor signaling induces behavioral quiescence in
RT Caenorhabditis elegans.";
RL Nat. Neurosci. 10:1300-1307(2007).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, AND MUTAGENESIS OF ALA-59; 216-GLN--ASP-522;
RP PRO-224 AND CYS-238.
RX PubMed=26156999; DOI=10.1523/jneurosci.0940-15.2015;
RA Correa P.A., Gruninger T., Garcia L.R.;
RT "DOP-2 D2-Like Receptor Regulates UNC-7 Innexins to Attenuate Recurrent
RT Sensory Motor Neurons during C. elegans Copulation.";
RL J. Neurosci. 35:9990-10004(2015).
CC -!- FUNCTION: Structural component of the gap junctions (By similarity).
CC Required for coordinated locomotion (PubMed:7681023). Probably by
CC regulating neuronal transmission downstream of lin-3 and receptor lin-
CC 23 and phospholipase plc-3 in ALA neurons, involved in the decrease in
CC pharyngeal pumping during the quiescent state that precedes each larval
CC molt (PubMed:17891142). Plays a role in regulating male mating behavior
CC by positively regulating inhibitory neuronal signaling transduction
CC between chemosensory and mechanosensory neurons, and thus ensuring
CC spicule insertion attempts are confined to the hermaphrodite vulva
CC during copulation (PubMed:26156999). {ECO:0000250|UniProtKB:O61715,
CC ECO:0000269|PubMed:17891142, ECO:0000269|PubMed:26156999,
CC ECO:0000269|PubMed:7681023}.
CC -!- SUBUNIT: Heterooligomer of unc-7 and unc-9. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000255|PROSITE-ProRule:PRU00351}. Cell junction, gap
CC junction.
CC -!- TISSUE SPECIFICITY: In hermaphrodites, expressed in all premotor and
CC motor neurons, and in tail neurons (PubMed:26156999). In males,
CC expressed in the anal depressor, gubernacular erector, gubernacular
CC retractor and anterior oblique muscles, SPC, PCA, PCB, HOA, ray, head
CC ganglion and ventral cord neurons (PubMed:26156999).
CC {ECO:0000269|PubMed:26156999}.
CC -!- DEVELOPMENTAL STAGE: Present in all developmental stages but most
CC abundant in L1-L3 larvae.
CC -!- DISRUPTION PHENOTYPE: Restores pharyngeal pumping but not impaired
CC locomotion in 33 percent of animals overexpressing lin-3.
CC {ECO:0000269|PubMed:17891142}.
CC -!- SIMILARITY: Belongs to the pannexin family. {ECO:0000255|PROSITE-
CC ProRule:PRU00351}.
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DR EMBL; Z19122; CAA79529.1; -; Transcribed_RNA.
DR EMBL; BX284606; CAA94607.1; -; Genomic_DNA.
DR PIR; T24027; T24027.
DR RefSeq; NP_001257255.1; NM_001270326.1.
DR AlphaFoldDB; Q03412; -.
DR SMR; Q03412; -.
DR BioGRID; 46505; 2.
DR STRING; 6239.R07D5.1a; -.
DR TCDB; 1.A.25.1.2; the gap junction-forming innexin (innexin) family.
DR EPD; Q03412; -.
DR PaxDb; Q03412; -.
DR EnsemblMetazoa; R07D5.1a.1; R07D5.1a.1; WBGene00006747.
DR EnsemblMetazoa; R07D5.1a.2; R07D5.1a.2; WBGene00006747.
DR GeneID; 181608; -.
DR UCSC; R07D5.1; c. elegans.
DR CTD; 181608; -.
DR WormBase; R07D5.1a; CE06279; WBGene00006747; unc-7.
DR eggNOG; ENOG502QVJ2; Eukaryota.
DR InParanoid; Q03412; -.
DR OMA; WHSGINL; -.
DR PhylomeDB; Q03412; -.
DR PRO; PR:Q03412; -.
DR Proteomes; UP000001940; Chromosome X.
DR Bgee; WBGene00006747; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR ExpressionAtlas; Q03412; baseline and differential.
DR GO; GO:0005911; C:cell-cell junction; IDA:WormBase.
DR GO; GO:0005921; C:gap junction; IMP:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0032589; C:neuron projection membrane; IDA:WormBase.
DR GO; GO:0005886; C:plasma membrane; IDA:WormBase.
DR GO; GO:0005243; F:gap junction channel activity; IDA:WormBase.
DR GO; GO:0055077; F:gap junction hemi-channel activity; ISS:UniProtKB.
DR GO; GO:1903351; P:cellular response to dopamine; IMP:UniProtKB.
DR GO; GO:0016264; P:gap junction assembly; TAS:WormBase.
DR GO; GO:0034220; P:ion transmembrane transport; IDA:WormBase.
DR GO; GO:0040011; P:locomotion; IMP:UniProtKB.
DR GO; GO:0018991; P:oviposition; IMP:WormBase.
DR GO; GO:0090325; P:regulation of locomotion involved in locomotory behavior; IMP:UniProtKB.
DR GO; GO:1902435; P:regulation of male mating behavior; IMP:UniProtKB.
DR GO; GO:1902847; P:regulation of neuronal signal transduction; IMP:UniProtKB.
DR GO; GO:0043051; P:regulation of pharyngeal pumping; IMP:WormBase.
DR GO; GO:0072347; P:response to anesthetic; IGI:WormBase.
DR GO; GO:0034609; P:spicule insertion; IMP:UniProtKB.
DR InterPro; IPR000990; Innexin.
DR PANTHER; PTHR11893; PTHR11893; 1.
DR Pfam; PF00876; Innexin; 1.
DR PRINTS; PR01262; INNEXIN.
DR PROSITE; PS51013; PANNEXIN; 1.
PE 1: Evidence at protein level;
KW Cell junction; Cell membrane; Gap junction; Ion channel; Ion transport;
KW Membrane; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..522
FT /note="Innexin unc-7"
FT /id="PRO_0000208517"
FT TRANSMEM 151..171
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT TRANSMEM 320..340
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT TRANSMEM 405..425
FT /note="Helical"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00351"
FT REGION 1..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 14..30
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 45..67
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 59
FT /note="A->T: In rg396; increases backwards movements.
FT Defects in male mating behavior with spicule protrusion
FT impairments. Impairs dopamine signaling."
FT /evidence="ECO:0000269|PubMed:26156999"
FT MUTAGEN 216..522
FT /note="Missing: In e5; defects in male mating behavior with
FT spicule protrusion impairments. Impairs dopamine
FT signaling."
FT /evidence="ECO:0000269|PubMed:26156999"
FT MUTAGEN 224
FT /note="P->L: In hs9; defects in male mating behavior with
FT spicule protrusion impairments. Impairs dopamine
FT signaling."
FT /evidence="ECO:0000269|PubMed:26156999"
FT MUTAGEN 238
FT /note="C->Y: In hs10; defects in male mating behavior with
FT spicule protrusion impairments. Impairs dopamine
FT signaling."
FT /evidence="ECO:0000269|PubMed:26156999"
SQ SEQUENCE 522 AA; 60121 MW; 950D7AFBA9F61120 CRC64;
MLGSSSNPEP PLLSRIIGVP PPPPPRAPTT ALVLRVPTVD SPSKKKQQPD TRNKYQETAL
RDKKTRTPLE KARHLDNLPS YQAQKLLDGS HQLRIDSHHV GSAGHGAGQG HGHKKEFGPA
MILYYLASAF RALYPRLDDD FVDKLNYYYT TTILASFALL VSAKQYVGFP IQCWVPATFT
DAMEQYTENY CWVQNTYWVP MQEDIPREIY SRRNRQIGYY QWVPFILAIE ALLFYVPCIL
WRGLLYWHSG INLQGLVQMA CDARLMDSEI KTRTVYTMAR HMQDEVQLTN IDRQGHSRSC
FSNLQLGANC GRHCGCYVTM LYIGIKVLYS ANVLLQFFLL NHLLGSNDLA YGFSLLKDLM
HAIEWEQTGM FPRVTLCDFE VRVLGNIHRH TVQCVLMINM FNEKIFLFLW FWFLTCGIVT
VCNTMYWILI MFIPSQGMSF VRKYLRVLPD HPAKPIADDV TLRKFTNNFL RKDGVFMLRM
ISTHAGELMS SELILALWQD FNNVDRSPTQ FWDAEHGQGT ID
