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UNC86_CAEEL
ID   UNC86_CAEEL             Reviewed;         467 AA.
AC   P13528; D7SFI2;
DT   01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 1.
DT   03-AUG-2022, entry version 174.
DE   RecName: Full=Transcription factor unc-86;
DE   AltName: Full=Uncoordinated protein 86;
GN   Name=unc-86; ORFNames=C30A5.7;
OS   Caenorhabditis elegans.
OC   Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC   Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC   Caenorhabditis.
OX   NCBI_TaxID=6239;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORMS A AND B).
RX   PubMed=2903797; DOI=10.1016/0092-8674(88)90132-8;
RA   Finney M., Ruvkun G., Horvitz H.R.;
RT   "The C. elegans cell lineage and differentiation gene unc-86 encodes a
RT   protein with a homeodomain and extended similarity to transcription
RT   factors.";
RL   Cell 55:757-769(1988).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC   STRAIN=Bristol N2;
RX   PubMed=7906398; DOI=10.1038/368032a0;
RA   Wilson R., Ainscough R., Anderson K., Baynes C., Berks M., Bonfield J.,
RA   Burton J., Connell M., Copsey T., Cooper J., Coulson A., Craxton M.,
RA   Dear S., Du Z., Durbin R., Favello A., Fraser A., Fulton L., Gardner A.,
RA   Green P., Hawkins T., Hillier L., Jier M., Johnston L., Jones M.,
RA   Kershaw J., Kirsten J., Laisster N., Latreille P., Lightning J., Lloyd C.,
RA   Mortimore B., O'Callaghan M., Parsons J., Percy C., Rifken L., Roopra A.,
RA   Saunders D., Shownkeen R., Sims M., Smaldon N., Smith A., Smith M.,
RA   Sonnhammer E., Staden R., Sulston J., Thierry-Mieg J., Thomas K.,
RA   Vaudin M., Vaughan K., Waterston R., Watson A., Weinstock L.,
RA   Wilkinson-Sproat J., Wohldman P.;
RT   "2.2 Mb of contiguous nucleotide sequence from chromosome III of C.
RT   elegans.";
RL   Nature 368:32-38(1994).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Bristol N2;
RX   PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG   The C. elegans sequencing consortium;
RT   "Genome sequence of the nematode C. elegans: a platform for investigating
RT   biology.";
RL   Science 282:2012-2018(1998).
RN   [4]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Bristol N2;
RX   PubMed=7237544; DOI=10.1016/0092-8674(81)90501-8;
RA   Chalfie M., Horvitz H.R., Sulston J.E.;
RT   "Mutations that lead to reiterations in the cell lineages of C. elegans.";
RL   Cell 24:59-69(1981).
RN   [5]
RP   FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP   STAGE.
RX   PubMed=2257628; DOI=10.1016/0092-8674(90)90493-x;
RA   Finney M., Ruvkun G.;
RT   "The unc-86 gene product couples cell lineage and cell identity in C.
RT   elegans.";
RL   Cell 63:895-905(1990).
RN   [6]
RP   FUNCTION.
RX   PubMed=1361171; DOI=10.1002/j.1460-2075.1992.tb05604.x;
RA   Xue D., Finney M., Ruvkun G., Chalfie M.;
RT   "Regulation of the mec-3 gene by the C.elegans homeoproteins UNC-86 and
RT   MEC-3.";
RL   EMBO J. 11:4969-4979(1992).
RN   [7]
RP   FUNCTION, AND SUBUNIT.
RC   STRAIN=Bristol N2;
RX   PubMed=9735371; DOI=10.1242/dev.125.20.4107;
RA   Duggan A., Ma C., Chalfie M.;
RT   "Regulation of touch receptor differentiation by the Caenorhabditis elegans
RT   mec-3 and unc-86 genes.";
RL   Development 125:4107-4119(1998).
RN   [8]
RP   FUNCTION, SUBUNIT, DISRUPTION PHENOTYPE, AND MUTAGENESIS OF PRO-270 AND
RP   LEU-320.
RX   PubMed=10899123; DOI=10.1093/emboj/19.14.3694;
RA   Rohrig S., Rockelein I., Donhauser R., Baumeister R.;
RT   "Protein interaction surface of the POU transcription factor UNC-86
RT   selectively used in touch neurons.";
RL   EMBO J. 19:3694-3703(2000).
RN   [9]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Bristol N2;
RX   PubMed=12135927; DOI=10.1242/dev.129.16.3901;
RA   Sze J.Y., Zhang S., Li J., Ruvkun G.;
RT   "The C. elegans POU-domain transcription factor UNC-86 regulates the tph-1
RT   tryptophan hydroxylase gene and neurite outgrowth in specific serotonergic
RT   neurons.";
RL   Development 129:3901-3911(2002).
RN   [10]
RP   FUNCTION, AND TISSUE SPECIFICITY.
RC   STRAIN=Bristol N2;
RX   PubMed=11959845; DOI=10.1101/gad.976002;
RA   Shaham S., Bargmann C.I.;
RT   "Control of neuronal subtype identity by the C. elegans ARID protein CFI-
RT   1.";
RL   Genes Dev. 16:972-983(2002).
RN   [11]
RP   FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX   PubMed=12883006; DOI=10.1073/pnas.1530752100;
RA   Sze J.Y., Ruvkun G.;
RT   "Activity of the Caenorhabditis elegans UNC-86 POU transcription factor
RT   modulates olfactory sensitivity.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:9560-9565(2003).
RN   [12]
RP   FUNCTION.
RC   STRAIN=Bristol N2;
RX   PubMed=16139210; DOI=10.1016/j.cub.2005.07.057;
RA   Kage E., Hayashi Y., Takeuchi H., Hirotsu T., Kunitomo H., Inoue T.,
RA   Arai H., Iino Y., Kubo T.;
RT   "MBR-1, a novel helix-turn-helix transcription factor, is required for
RT   pruning excessive neurites in Caenorhabditis elegans.";
RL   Curr. Biol. 15:1554-1559(2005).
RN   [13]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=Bristol N2;
RX   PubMed=21656875; DOI=10.1002/dvdy.22667;
RA   Olsson-Carter K., Slack F.J.;
RT   "The POU transcription factor UNC-86 controls the timing and ventral
RT   guidance of Caenorhabditis elegans axon growth.";
RL   Dev. Dyn. 240:1815-1825(2011).
RN   [14]
RP   FUNCTION.
RC   STRAIN=Bristol N2;
RX   PubMed=23932402; DOI=10.1016/j.cub.2013.06.058;
RA   Schroeder N.E., Androwski R.J., Rashid A., Lee H., Lee J., Barr M.M.;
RT   "Dauer-specific dendrite arborization in C. elegans is regulated by KPC-
RT   1/Furin.";
RL   Curr. Biol. 23:1527-1535(2013).
RN   [15]
RP   FUNCTION, DNA-BINDING, AND DISRUPTION PHENOTYPE.
RX   PubMed=24353061; DOI=10.1242/dev.099721;
RA   Zhang F., Bhattacharya A., Nelson J.C., Abe N., Gordon P.,
RA   Lloret-Fernandez C., Maicas M., Flames N., Mann R.S., Colon-Ramos D.A.,
RA   Hobert O.;
RT   "The LIM and POU homeobox genes ttx-3 and unc-86 act as terminal selectors
RT   in distinct cholinergic and serotonergic neuron types.";
RL   Development 141:422-435(2014).
CC   -!- FUNCTION: Transcription factor required for correct cell fate
CC       determination and differentiation in diverse neuronal cell lineages
CC       where it plays a role in specifying the fate of daughter cells during
CC       cell divisions (PubMed:7237544, PubMed:2257628). Involved in sensory
CC       neuron production and function (PubMed:1361171, PubMed:9735371,
CC       PubMed:10899123). Binds both alone and with mec-3 to the mec-3 promoter
CC       to initiate and maintain mec-3 expression which is required for sensory
CC       neuron differentiation (PubMed:1361171, PubMed:9735371). In addition,
CC       binds both alone and with mec-3 to the promoters of mec-4 and mec-7
CC       which act to regulate sensory neuron function (PubMed:9735371,
CC       PubMed:10899123). Involved in determining the identity of the
CC       serotonergic NSM neurons and the cholinergic IL2 sensory and URA motor
CC       neurons (PubMed:24353061). Promotes expression of the cfi-1
CC       transcription factor in the URA and IL2 neurons which in turn activates
CC       normal URA and IL2 gene expression (PubMed:24353061, PubMed:11959845).
CC       Regulates expression of a number of genes in NSM neurons including bas-
CC       1, cat-1, dop-3, mgl-3, nlp-13, scd-2 and ptps-1 (PubMed:24353061). In
CC       the IL2 neurons, required for expression of cho-1, gcy-19, klp-6, lag-
CC       2, unc-5 and unc-17 (PubMed:24353061). Promotes expression of pkd-2 in
CC       the male-specific CEM head neurons (PubMed:11959845). Required for
CC       dauer-specific branching of IL2Q neurons and nictation behavior
CC       (PubMed:23932402). Controls both the timing and direction of axon
CC       outgrowth in HSN neurons (PubMed:21656875). Plays a role in serotonin
CC       production by regulating expression of the tryptophan hydrolase tph-1
CC       which catalyzes serotonin synthesis, in the AIM, NSM, HSN and RIH
CC       neurons (PubMed:12135927). Involved in regulation of lin-11 expression
CC       in the AIZ interneurons, the major interneurons of the olfactory
CC       pathway, and is required for odortaxis behavior (PubMed:12883006).
CC       Involved in neurite pruning between AIM neurons during larval
CC       development by regulating the expression of transcription factor mbr-1
CC       (PubMed:16139210). Required for correct localization of unc-40
CC       (PubMed:24353061). {ECO:0000269|PubMed:10899123,
CC       ECO:0000269|PubMed:11959845, ECO:0000269|PubMed:12135927,
CC       ECO:0000269|PubMed:12883006, ECO:0000269|PubMed:1361171,
CC       ECO:0000269|PubMed:16139210, ECO:0000269|PubMed:21656875,
CC       ECO:0000269|PubMed:2257628, ECO:0000269|PubMed:23932402,
CC       ECO:0000269|PubMed:24353061, ECO:0000269|PubMed:7237544,
CC       ECO:0000269|PubMed:9735371}.
CC   -!- SUBUNIT: Interacts with mec-3; the heterooligomer binds to the
CC       promoters of mec-3, mec-4 and mec-7. {ECO:0000269|PubMed:10899123,
CC       ECO:0000269|PubMed:9735371}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC       ECO:0000255|PROSITE-ProRule:PRU00530, ECO:0000269|PubMed:2257628}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=b;
CC         IsoId=P13528-1; Sequence=Displayed;
CC       Name=a;
CC         IsoId=P13528-2; Sequence=VSP_002343, VSP_002344;
CC   -!- TISSUE SPECIFICITY: Specific to neurons and neuroblasts. Expressed in
CC       CEM head neurons and in IL2, URA, URB, URX and URY neurons. Not
CC       expressed in olfactory sensory neurons but expressed in AIZ
CC       interneurons. {ECO:0000269|PubMed:11959845,
CC       ECO:0000269|PubMed:12883006, ECO:0000269|PubMed:2257628}.
CC   -!- DEVELOPMENTAL STAGE: First appears during embryogenesis and persists
CC       throughout adulthood. {ECO:0000269|PubMed:2257628}.
CC   -!- DISRUPTION PHENOTYPE: In a number of neuronal cell lineages, one of the
CC       two daughter cells of a division fails to assume its normal fate,
CC       retaining instead the fate of its parent cell. Altered gene expression
CC       in a number of neuron types including reduced cat-1 expression in AIM,
CC       NSM, HSN and RIH neurons, loss of cat-4 expression in HSN neurons, loss
CC       of expression of dop-3 and mgl-3 and reduced expression of bas-1, nlp-
CC       13, ptpa-1 and scd-2 in NSM neurons, loss of expression of gcy-19, klp-
CC       6, lag-2 and unc-17 and reduced expression of cho-1 and unc-5 in IL2
CC       neurons, and loss of tph-1 expression in AIM, NSM, HSN and RIH neurons
CC       which leads to defects in serotonin production. Defective motility
CC       along with defects in IL2Q dendritic arborization and nictation.
CC       Abnormal neurite outgrowth in NSM neurons. Defective response to odor
CC       attractants but odor repellants are avoided normally. Penetrant ventral
CC       axon growth defects in HSN motor neurons with extension of processes in
CC       L1, three stages earlier than wild-type. Incorrect unc-40 localization.
CC       Defective egg-laying characterized by retention of substantially more
CC       eggs in the uterus of second-day animals than the wild-type.
CC       {ECO:0000269|PubMed:10899123, ECO:0000269|PubMed:12135927,
CC       ECO:0000269|PubMed:12883006, ECO:0000269|PubMed:21656875,
CC       ECO:0000269|PubMed:24353061, ECO:0000269|PubMed:7237544}.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-4
CC       subfamily. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCD62655.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC       Sequence=CCD62656.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; M22363; AAA28158.1; -; Genomic_DNA.
DR   EMBL; M22363; AAA28159.1; -; Genomic_DNA.
DR   EMBL; FO080290; CCD62655.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; FO080290; CCD62656.1; ALT_SEQ; Genomic_DNA.
DR   PIR; A30042; A30042.
DR   PIR; S44778; S44778.
DR   RefSeq; NP_001021191.2; NM_001026020.4.
DR   RefSeq; NP_001309473.1; NM_001322687.1.
DR   AlphaFoldDB; P13528; -.
DR   SMR; P13528; -.
DR   BioGRID; 41362; 2.
DR   IntAct; P13528; 1.
DR   STRING; 6239.C30A5.7b; -.
DR   EPD; P13528; -.
DR   PaxDb; P13528; -.
DR   PRIDE; P13528; -.
DR   EnsemblMetazoa; C30A5.7a.1; C30A5.7a.1; WBGene00006818.
DR   EnsemblMetazoa; C30A5.7b.1; C30A5.7b.1; WBGene00006818.
DR   GeneID; 176157; -.
DR   KEGG; cel:CELE_C30A5.7; -.
DR   UCSC; C30A5.7b; c. elegans. [P13528-1]
DR   CTD; 176157; -.
DR   WormBase; C30A5.7a; CE51471; WBGene00006818; unc-86.
DR   WormBase; C30A5.7b; CE45067; WBGene00006818; unc-86.
DR   eggNOG; KOG1168; Eukaryota.
DR   GeneTree; ENSGT00940000169264; -.
DR   InParanoid; P13528; -.
DR   OrthoDB; 929123at2759; -.
DR   PhylomeDB; P13528; -.
DR   SignaLink; P13528; -.
DR   PRO; PR:P13528; -.
DR   Proteomes; UP000001940; Chromosome III.
DR   Bgee; WBGene00006818; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR   GO; GO:0005634; C:nucleus; IDA:WormBase.
DR   GO; GO:0090575; C:RNA polymerase II transcription regulator complex; IDA:WormBase.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IDA:WormBase.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IDA:WormBase.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:WormBase.
DR   GO; GO:0008134; F:transcription factor binding; IPI:WormBase.
DR   GO; GO:0007638; P:mechanosensory behavior; IMP:WormBase.
DR   GO; GO:0048666; P:neuron development; IMP:WormBase.
DR   GO; GO:0048665; P:neuron fate specification; IMP:WormBase.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:WormBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0009612; P:response to mechanical stimulus; IMP:WormBase.
DR   GO; GO:0007608; P:sensory perception of smell; IEA:UniProtKB-KW.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.260.40; -; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF47413; SSF47413; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
PE   1: Evidence at protein level;
KW   Alternative splicing; DNA-binding; Homeobox; Neurogenesis; Nucleus;
KW   Olfaction; Reference proteome; Sensory transduction; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..467
FT                   /note="Transcription factor unc-86"
FT                   /id="PRO_0000100780"
FT   DOMAIN          265..342
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        363..422
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   MOTIF           145..154
FT                   /note="POU-IV box"
FT   VAR_SEQ         129..134
FT                   /note="FNNSLF -> DFILFI (in isoform a)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_002343"
FT   VAR_SEQ         135..172
FT                   /note="Missing (in isoform a)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_002344"
FT   MUTAGEN         270
FT                   /note="P->S: In u168; defective mechanosensation, lack of
FT                   interaction with mec-3 and lack of maintained mec-3
FT                   expression. No egg-laying or Q neuroblast lineage defects."
FT                   /evidence="ECO:0000269|PubMed:10899123"
FT   MUTAGEN         320
FT                   /note="L->F: In u5; defective mechanosensation, lack of
FT                   interaction with mec-3 and lack of maintained mec-3
FT                   expression. No egg-laying or Q neuroblast lineage defects."
FT                   /evidence="ECO:0000269|PubMed:10899123"
SQ   SEQUENCE   467 AA;  52315 MW;  86C3507584C91AC2 CRC64;
     MEKAHRFRLP FCSFFPVPLL VSVLIFHHSA PFLIQLFFPS PLFNPLLRPS KISRGSENGA
     CTSHSTLQRT RKIIQWELPK RGGDQDIGDP RPFRIHLSPP SFKVPLFSTD MQNTAPVPTT
     TTASKMQPFN NSLFGSFDDP ILNARAAQVA LADIDVKNVP QLTNPLMRPH DMFSYSNYFS
     GIHDTSAATN IYQGLPSSSE PFDASVVVPT SSDDQMTPLQ QVMAMQQSYG APPPFQYNMT
     HPFSTTSIAS SNNLARYPIA PPTSDMDTDP RQLETFAEHF KQRRIKLGVT QADVGKALAH
     LKMPGVGSLS QSTICRFESL TLSHNNMVAL KPILHSWLEK AEEAMKQKDT IGDINGILPN
     TDKKRKRTSI AAPEKRELEQ FFKQQPRPSG ERIASIADRL DLKKNVVRVW FCNQRQKQKR
     DFRSQFRARS AAAVMGPRVM PVLNGNNSNN NLKQGQTTYN GLPGFFD
 
 
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