UNE10_ARATH
ID UNE10_ARATH Reviewed; 399 AA.
AC Q8GZ38; O81306; Q8S3F4;
DT 16-DEC-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Transcription factor UNE10;
DE AltName: Full=Basic helix-loop-helix protein 16;
DE Short=AtbHLH16;
DE Short=bHLH 16;
DE AltName: Full=Protein UNFERTILIZED EMBRYO SAC 10;
DE AltName: Full=Transcription factor EN 108;
DE AltName: Full=bHLH transcription factor bHLH016;
GN Name=UNE10; Synonyms=BHLH16, EN108; OrderedLocusNames=At4g00050;
GN ORFNames=F6N15.11;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, INDUCTION BY COLD, GENE
RP FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia; TISSUE=Flower;
RX PubMed=12679534; DOI=10.1093/molbev/msg088;
RA Heim M.A., Jakoby M., Werber M., Martin C., Weisshaar B., Bailey P.C.;
RT "The basic helix-loop-helix transcription factor family in plants: a
RT genome-wide study of protein structure and functional diversity.";
RL Mol. Biol. Evol. 20:735-747(2003).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617198; DOI=10.1038/47134;
RA Mayer K.F.X., Schueller C., Wambutt R., Murphy G., Volckaert G., Pohl T.,
RA Duesterhoeft A., Stiekema W., Entian K.-D., Terryn N., Harris B.,
RA Ansorge W., Brandt P., Grivell L.A., Rieger M., Weichselgartner M.,
RA de Simone V., Obermaier B., Mache R., Mueller M., Kreis M., Delseny M.,
RA Puigdomenech P., Watson M., Schmidtheini T., Reichert B., Portetelle D.,
RA Perez-Alonso M., Boutry M., Bancroft I., Vos P., Hoheisel J.,
RA Zimmermann W., Wedler H., Ridley P., Langham S.-A., McCullagh B.,
RA Bilham L., Robben J., van der Schueren J., Grymonprez B., Chuang Y.-J.,
RA Vandenbussche F., Braeken M., Weltjens I., Voet M., Bastiaens I., Aert R.,
RA Defoor E., Weitzenegger T., Bothe G., Ramsperger U., Hilbert H., Braun M.,
RA Holzer E., Brandt A., Peters S., van Staveren M., Dirkse W., Mooijman P.,
RA Klein Lankhorst R., Rose M., Hauf J., Koetter P., Berneiser S., Hempel S.,
RA Feldpausch M., Lamberth S., Van den Daele H., De Keyser A., Buysshaert C.,
RA Gielen J., Villarroel R., De Clercq R., van Montagu M., Rogers J.,
RA Cronin A., Quail M.A., Bray-Allen S., Clark L., Doggett J., Hall S.,
RA Kay M., Lennard N., McLay K., Mayes R., Pettett A., Rajandream M.A.,
RA Lyne M., Benes V., Rechmann S., Borkova D., Bloecker H., Scharfe M.,
RA Grimm M., Loehnert T.-H., Dose S., de Haan M., Maarse A.C., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Fartmann B., Granderath K., Dauner D.,
RA Herzl A., Neumann S., Argiriou A., Vitale D., Liguori R., Piravandi E.,
RA Massenet O., Quigley F., Clabauld G., Muendlein A., Felber R., Schnabl S.,
RA Hiller R., Schmidt W., Lecharny A., Aubourg S., Chefdor F., Cooke R.,
RA Berger C., Monfort A., Casacuberta E., Gibbons T., Weber N., Vandenbol M.,
RA Bargues M., Terol J., Torres A., Perez-Perez A., Purnelle B., Bent E.,
RA Johnson S., Tacon D., Jesse T., Heijnen L., Schwarz S., Scholler P.,
RA Heber S., Francs P., Bielke C., Frishman D., Haase D., Lemcke K.,
RA Mewes H.-W., Stocker S., Zaccaria P., Bevan M., Wilson R.K.,
RA de la Bastide M., Habermann K., Parnell L., Dedhia N., Gnoj L., Schutz K.,
RA Huang E., Spiegel L., Sekhon M., Murray J., Sheet P., Cordes M.,
RA Abu-Threideh J., Stoneking T., Kalicki J., Graves T., Harmon G.,
RA Edwards J., Latreille P., Courtney L., Cloud J., Abbott A., Scott K.,
RA Johnson D., Minx P., Bentley D., Fulton B., Miller N., Greco T., Kemp K.,
RA Kramer J., Fulton L., Mardis E., Dante M., Pepin K., Hillier L.W.,
RA Nelson J., Spieth J., Ryan E., Andrews S., Geisel C., Layman D., Du H.,
RA Ali J., Berghoff A., Jones K., Drone K., Cotton M., Joshu C., Antonoiu B.,
RA Zidanic M., Strong C., Sun H., Lamar B., Yordan C., Ma P., Zhong J.,
RA Preston R., Vil D., Shekher M., Matero A., Shah R., Swaby I.K.,
RA O'Shaughnessy A., Rodriguez M., Hoffman J., Till S., Granat S., Shohdy N.,
RA Hasegawa A., Hameed A., Lodhi M., Johnson A., Chen E., Marra M.A.,
RA Martienssen R., McCombie W.R.;
RT "Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.";
RL Nature 402:769-777(1999).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11910074; DOI=10.1126/science.1071006;
RA Seki M., Narusaka M., Kamiya A., Ishida J., Satou M., Sakurai T.,
RA Nakajima M., Enju A., Akiyama K., Oono Y., Muramatsu M., Hayashizaki Y.,
RA Kawai J., Carninci P., Itoh M., Ishii Y., Arakawa T., Shibata K.,
RA Shinagawa A., Shinozaki K.;
RT "Functional annotation of a full-length Arabidopsis cDNA collection.";
RL Science 296:141-145(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Shinn P., Chen H., Kim C.J., Quinitio C., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP GENE FAMILY.
RX PubMed=12897250; DOI=10.1105/tpc.013839;
RA Toledo-Ortiz G., Huq E., Quail P.H.;
RT "The Arabidopsis basic/helix-loop-helix transcription factor family.";
RL Plant Cell 15:1749-1770(2003).
RN [7]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14600211; DOI=10.1105/tpc.151140;
RA Bailey P.C., Martin C., Toledo-Ortiz G., Quail P.H., Huq E., Heim M.A.,
RA Jakoby M., Werber M., Weisshaar B.;
RT "Update on the basic helix-loop-helix transcription factor gene family in
RT Arabidopsis thaliana.";
RL Plant Cell 15:2497-2502(2003).
RN [8]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=15634699; DOI=10.1242/dev.01595;
RA Pagnussat G.C., Yu H.-J., Ngo Q.A., Rajani S., Mayalagu S., Johnson C.S.,
RA Capron A., Xie L.-F., Ye D., Sundaresan V.;
RT "Genetic and molecular identification of genes required for female
RT gametophyte development and function in Arabidopsis.";
RL Development 132:603-614(2005).
CC -!- FUNCTION: Required during the fertilization of ovules by pollen.
CC {ECO:0000269|PubMed:15634699}.
CC -!- SUBUNIT: Homodimer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00981}.
CC -!- TISSUE SPECIFICITY: Expressed in stems and flowers.
CC {ECO:0000269|PubMed:12679534}.
CC -!- INDUCTION: By cold treatment. {ECO:0000269|PubMed:12679534}.
CC -!- DISRUPTION PHENOTYPE: Plants exhibit unfertilized ovules but normal
CC pollen tube attraction. {ECO:0000269|PubMed:15634699}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAC19308.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=CAB80763.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF488561; AAM10933.1; -; mRNA.
DR EMBL; AF069299; AAC19308.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AL161471; CAB80763.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002687; AEE81818.1; -; Genomic_DNA.
DR EMBL; AK117229; BAC41905.1; -; mRNA.
DR EMBL; BT025971; ABG25060.1; -; mRNA.
DR PIR; T01333; T01333.
DR RefSeq; NP_191916.3; NM_116222.4.
DR AlphaFoldDB; Q8GZ38; -.
DR SMR; Q8GZ38; -.
DR BioGRID; 13464; 3.
DR IntAct; Q8GZ38; 1.
DR STRING; 3702.AT4G00050.1; -.
DR PaxDb; Q8GZ38; -.
DR PRIDE; Q8GZ38; -.
DR EnsemblPlants; AT4G00050.1; AT4G00050.1; AT4G00050.
DR GeneID; 828175; -.
DR Gramene; AT4G00050.1; AT4G00050.1; AT4G00050.
DR KEGG; ath:AT4G00050; -.
DR Araport; AT4G00050; -.
DR TAIR; locus:2126876; AT4G00050.
DR eggNOG; ENOG502QUQP; Eukaryota.
DR HOGENOM; CLU_044659_0_0_1; -.
DR InParanoid; Q8GZ38; -.
DR OMA; PMASWDG; -.
DR OrthoDB; 1153539at2759; -.
DR PhylomeDB; Q8GZ38; -.
DR PRO; PR:Q8GZ38; -.
DR Proteomes; UP000006548; Chromosome 4.
DR ExpressionAtlas; Q8GZ38; baseline and differential.
DR Genevisible; Q8GZ38; AT.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0009506; C:plasmodesma; HDA:TAIR.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046983; F:protein dimerization activity; IEA:InterPro.
DR GO; GO:0009704; P:de-etiolation; IMP:TAIR.
DR GO; GO:0009567; P:double fertilization forming a zygote and endosperm; IMP:TAIR.
DR GO; GO:0009959; P:negative gravitropism; IMP:TAIR.
DR GO; GO:0010187; P:negative regulation of seed germination; IMP:TAIR.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; TAS:TAIR.
DR GO; GO:0009637; P:response to blue light; IMP:TAIR.
DR GO; GO:0010218; P:response to far red light; IMP:TAIR.
DR GO; GO:0009416; P:response to light stimulus; IMP:TAIR.
DR GO; GO:0009845; P:seed germination; IMP:TAIR.
DR GO; GO:0090351; P:seedling development; IGI:TAIR.
DR GO; GO:0006351; P:transcription, DNA-templated; IDA:TAIR.
DR Gene3D; 4.10.280.10; -; 1.
DR InterPro; IPR031066; bHLH_ALC-like_plant.
DR InterPro; IPR011598; bHLH_dom.
DR InterPro; IPR036638; HLH_DNA-bd_sf.
DR PANTHER; PTHR45855; PTHR45855; 1.
DR Pfam; PF00010; HLH; 1.
DR SMART; SM00353; HLH; 1.
DR SUPFAM; SSF47459; SSF47459; 1.
DR PROSITE; PS50888; BHLH; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..399
FT /note="Transcription factor UNE10"
FT /id="PRO_0000358830"
FT DOMAIN 213..262
FT /note="bHLH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00981"
FT REGION 119..158
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 173..228
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 119..134
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 177..205
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 390
FT /note="Q -> R (in Ref. 1; AAM10933)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 399 AA; 43365 MW; CEF7F585022AB0FC CRC64;
MSQCVPNCHI DDTPAAATTT VRSTTAADIP ILDYEVAELT WENGQLGLHG LGPPRVTASS
TKYSTGAGGT LESIVDQATR LPNPKPTDEL VPWFHHRSSR AAMAMDALVP CSNLVHEQQS
KPGGVGSTRV GSCSDGRTMG GGKRARVAPE WSGGGSQRLT MDTYDVGFTS TSMGSHDNTI
DDHDSVCHSR PQMEDEEEKK AGGKSSVSTK RSRAAAIHNQ SERKRRDKIN QRMKTLQKLV
PNSSKTDKAS MLDEVIEYLK QLQAQVSMMS RMNMPSMMLP MAMQQQQQLQ MSLMSNPMGL
GMGMGMPGLG LLDLNSMNRA AASAPNIHAN MMPNPFLPMN CPSWDASSND SRFQSPLIPD
PMSAFLACST QPTTMEAYSR MATLYQQMQQ QLPPPSNPK
