ID   UNGI_BPPB2              Reviewed;          84 AA.
AC   P14739;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=Uracil-DNA glycosylase inhibitor;
GN   Name=UGI;
OS   Bacillus phage PBS2 (Bacteriophage PBS2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Myoviridae.
OX   NCBI_TaxID=10684;
OH   NCBI_TaxID=1423; Bacillus subtilis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-5.
RX   PubMed=2492016; DOI=10.1016/s0021-9258(19)85067-6;
RA   Wang Z., Mosbaugh D.W.;
RT   "Uracil-DNA glycosylase inhibitor gene of bacteriophage PBS2 encodes a
RT   binding protein specific for uracil-DNA glycosylase.";
RL   J. Biol. Chem. 264:1163-1171(1989).
RN   [2]
RP   STRUCTURE BY NMR.
RX   PubMed=9261156; DOI=10.1074/jbc.272.34.21408;
RA   Lundquist A.J., Beger R.D., Bennett S.E., Bolton P.H., Mosbaugh D.W.;
RT   "Site-directed mutagenesis and characterization of uracil-DNA glycosylase
RT   inhibitor protein. Role of specific carboxylic amino acids in complex
RT   formation with Escherichia coli uracil-DNA glycosylase.";
RL   J. Biol. Chem. 272:21408-21419(1997).
RN   [3]
RP   X-RAY CRYSTALLOGRAPHY (3.2 ANGSTROMS).
RX   PubMed=9776748; DOI=10.1093/nar/26.21.4880;
RA   Ravishankar R., Sagar M.B., Roy S., Purnapatre K., Handa P., Varshney U.,
RA   Vijayan M.;
RT   "X-ray analysis of a complex of Escherichia coli uracil DNA glycosylase
RT   (EcUDG) with a proteinaceous inhibitor. The structure elucidation of a
RT   prokaryotic UDG.";
RL   Nucleic Acids Res. 26:4880-4887(1998).
RN   [4]
RP   X-RAY CRYSTALLOGRAPHY (1.55 ANGSTROMS).
RX   PubMed=10080896; DOI=10.1006/jmbi.1999.2605;
RA   Putnam C.D., Shroyer M.J.N., Lundquist A.J., Mol C.D., Arvai A.S.,
RA   Mosbaugh D.W., Tainer J.A.;
RT   "Protein mimicry of DNA from crystal structures of the uracil-DNA
RT   glycosylase inhibitor protein and its complex with Escherichia coli uracil-
RT   DNA glycosylase.";
RL   J. Mol. Biol. 287:331-346(1999).
CC   -!- FUNCTION: This protein binds specifically and reversibly to the host
CC       uracil-DNA glycosylase, preventing removal of uracil residues from PBS2
CC       DNA by the host uracil-excision repair system.
CC   -!- INTERACTION:
CC       P14739; P12295: ung; Xeno; NbExp=4; IntAct=EBI-1025973, EBI-559403;
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DR   EMBL; J04434; AAA91582.1; -; Genomic_DNA.
DR   PIR; A32206; QIBPS2.
DR   PDB; 1EUI; X-ray; 3.20 A; C/D=1-84.
DR   PDB; 1LQG; X-ray; 2.90 A; C/D=1-84.
DR   PDB; 1LQM; X-ray; 3.20 A; B/D/F/H=1-84.
DR   PDB; 1UDI; X-ray; 2.70 A; I=2-84.
DR   PDB; 1UGH; X-ray; 1.90 A; I=3-84.
DR   PDB; 1UGI; X-ray; 1.55 A; A/B/C/D/E/F/G/H=1-84.
DR   PDB; 1UUG; X-ray; 2.40 A; B/D=1-84.
DR   PDB; 2J8X; X-ray; 2.30 A; B/D=1-84.
DR   PDB; 2UGI; X-ray; 2.20 A; A/B=1-84.
DR   PDB; 2UUG; X-ray; 2.60 A; C/D=1-84.
DR   PDB; 2ZHX; X-ray; 3.10 A; B/D/F/H/J/L/N=1-84.
DR   PDB; 4LYL; X-ray; 1.93 A; B/D/F/H/J/L/N/P=1-84.
DR   PDB; 6LYD; X-ray; 2.60 A; I=2-84.
DR   PDB; 6LYE; X-ray; 3.10 A; I=2-84.
DR   PDBsum; 1EUI; -.
DR   PDBsum; 1LQG; -.
DR   PDBsum; 1LQM; -.
DR   PDBsum; 1UDI; -.
DR   PDBsum; 1UGH; -.
DR   PDBsum; 1UGI; -.
DR   PDBsum; 1UUG; -.
DR   PDBsum; 2J8X; -.
DR   PDBsum; 2UGI; -.
DR   PDBsum; 2UUG; -.
DR   PDBsum; 2ZHX; -.
DR   PDBsum; 4LYL; -.
DR   PDBsum; 6LYD; -.
DR   PDBsum; 6LYE; -.
DR   BMRB; P14739; -.
DR   SMR; P14739; -.
DR   IntAct; P14739; 2.
DR   MINT; P14739; -.
DR   DrugBank; DB03366; Imidazole.
DR   SABIO-RK; P14739; -.
DR   EvolutionaryTrace; P14739; -.
DR   Gene3D; 3.10.450.20; -; 1.
DR   InterPro; IPR024062; Phage_PBS2_Ugi.
DR   InterPro; IPR043805; Ugi.
DR   Pfam; PF18880; UDI; 1.
DR   SUPFAM; SSF54441; SSF54441; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Direct protein sequencing.
FT   CHAIN           1..84
FT                   /note="Uracil-DNA glycosylase inhibitor"
FT                   /id="PRO_0000065722"
FT   HELIX           3..12
FT                   /evidence="ECO:0007829|PDB:1UGI"
FT   STRAND          22..24
FT                   /evidence="ECO:0007829|PDB:1UGI"
FT   HELIX           26..33
FT                   /evidence="ECO:0007829|PDB:1UGI"
FT   STRAND          41..48
FT                   /evidence="ECO:0007829|PDB:1UGI"
FT   TURN            49..52
FT                   /evidence="ECO:0007829|PDB:1UGI"
FT   STRAND          53..60
FT                   /evidence="ECO:0007829|PDB:1UGI"
FT   TURN            62..64
FT                   /evidence="ECO:0007829|PDB:1UGI"
FT   STRAND          67..73
FT                   /evidence="ECO:0007829|PDB:1UGI"
FT   STRAND          75..77
FT                   /evidence="ECO:0007829|PDB:6LYE"
FT   STRAND          79..83
FT                   /evidence="ECO:0007829|PDB:1UGI"
SQ   SEQUENCE   84 AA;  9476 MW;  C07DCF9DB101BC2A CRC64;
     MTNLSDIIEK ETGKQLVIQE SILMLPEEVE EVIGNKPESD ILVHTAYDES TDENVMLLTS
     DAPEYKPWAL VIQDSNGENK IKML