ID   UNG_BORBU               Reviewed;         223 AA.
AC   O51082; Q44841;
DT   15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-1998, sequence version 1.
DT   25-MAY-2022, entry version 122.
DE   RecName: Full=Uracil-DNA glycosylase;
DE            Short=UDG;
DE            EC=3.2.2.27;
GN   Name=ung; Synonyms=udg; OrderedLocusNames=BB_0053;
OS   Borreliella burgdorferi (strain ATCC 35210 / DSM 4680 / CIP 102532 / B31)
OS   (Borrelia burgdorferi).
OC   Bacteria; Spirochaetes; Spirochaetales; Borreliaceae; Borreliella.
OX   NCBI_TaxID=224326;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RX   PubMed=9403685; DOI=10.1038/37551;
RA   Fraser C.M., Casjens S., Huang W.M., Sutton G.G., Clayton R.A.,
RA   Lathigra R., White O., Ketchum K.A., Dodson R.J., Hickey E.K., Gwinn M.L.,
RA   Dougherty B.A., Tomb J.-F., Fleischmann R.D., Richardson D.L.,
RA   Peterson J.D., Kerlavage A.R., Quackenbush J., Salzberg S.L., Hanson M.,
RA   van Vugt R., Palmer N., Adams M.D., Gocayne J.D., Weidman J.F.,
RA   Utterback T.R., Watthey L., McDonald L.A., Artiach P., Bowman C.,
RA   Garland S.A., Fujii C., Cotton M.D., Horst K., Roberts K.M., Hatch B.,
RA   Smith H.O., Venter J.C.;
RT   "Genomic sequence of a Lyme disease spirochaete, Borrelia burgdorferi.";
RL   Nature 390:580-586(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-80.
RC   STRAIN=ATCC 35210 / DSM 4680 / CIP 102532 / B31;
RA   Gebbia J.A., Backenson P.B., Anda P., Coleman J.L., Benach J.L.;
RL   Submitted (JUN-1996) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Excises uracil residues from the DNA which can arise as a
CC       result of misincorporation of dUMP residues by DNA polymerase or due to
CC       deamination of cytosine. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes single-stranded DNA or mismatched double-stranded
CC         DNA and polynucleotides, releasing free uracil.; EC=3.2.2.27;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily.
CC       UNG family. {ECO:0000305}.
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DR   EMBL; AE000783; AAC66430.1; -; Genomic_DNA.
DR   EMBL; U57684; AAB53935.1; -; Genomic_DNA.
DR   PIR; E70106; E70106.
DR   RefSeq; NP_212187.1; NC_001318.1.
DR   RefSeq; WP_002656594.1; NC_001318.1.
DR   AlphaFoldDB; O51082; -.
DR   SMR; O51082; -.
DR   STRING; 224326.BB_0053; -.
DR   PRIDE; O51082; -.
DR   EnsemblBacteria; AAC66430; AAC66430; BB_0053.
DR   GeneID; 56568164; -.
DR   KEGG; bbu:BB_0053; -.
DR   PATRIC; fig|224326.49.peg.451; -.
DR   HOGENOM; CLU_032162_3_0_12; -.
DR   OMA; PDNGYLM; -.
DR   Proteomes; UP000001807; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004844; F:uracil DNA N-glycosylase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006284; P:base-excision repair; IEA:UniProtKB-UniRule.
DR   CDD; cd10027; UDG-F1-like; 1.
DR   Gene3D; 3.40.470.10; -; 1.
DR   HAMAP; MF_00148; UDG; 1.
DR   InterPro; IPR002043; UDG_fam1.
DR   InterPro; IPR018085; Ura-DNA_Glyclase_AS.
DR   InterPro; IPR005122; Uracil-DNA_glycosylase-like.
DR   InterPro; IPR036895; Uracil-DNA_glycosylase-like_sf.
DR   PANTHER; PTHR11264; PTHR11264; 1.
DR   Pfam; PF03167; UDG; 1.
DR   SMART; SM00986; UDG; 1.
DR   SUPFAM; SSF52141; SSF52141; 1.
DR   TIGRFAMs; TIGR00628; ung; 1.
DR   PROSITE; PS00130; U_DNA_GLYCOSYLASE; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; DNA damage; DNA repair; Hydrolase; Reference proteome.
FT   CHAIN           1..223
FT                   /note="Uracil-DNA glycosylase"
FT                   /id="PRO_0000176071"
FT   ACT_SITE        67
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        32
FT                   /note="E -> D (in Ref. 2; AAB53935)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        73..79
FT                   /note="NQANGLA -> KPSXWTC (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   223 AA;  25561 MW;  C5B72DC10B96C487 CRC64;
     MKVKIEESWK EVLNNEFNKE YFKKLVKFIK HEYKTKNGKI FPPPKLIFNA FNSLPFKDIK
     VVIIGQDPYH GKNQANGLAF SVDSKIKIPP SLQNIFKEIE KSLKIKTIPN GDLKRWAIQG
     VFLINTILTV EEGKPSSHKA IGWEIFTDEV IKIISKNLKN IVFMLWGNLA RSKKGLIDPT
     KHLILETSHP SPYSANNGFL GSNHFSSALD YLKKHNKNII NFQ