UNG_MYCTU
ID UNG_MYCTU Reviewed; 227 AA.
AC P9WFQ9; L0TBF3; P67071; P95119;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 38.
DE RecName: Full=Uracil-DNA glycosylase;
DE Short=UDG;
DE EC=3.2.2.27;
GN Name=ung; OrderedLocusNames=Rv2976c; ORFNames=MTCY349.11;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- FUNCTION: Excises uracil residues from the DNA which can arise as a
CC result of misincorporation of dUMP residues by DNA polymerase or due to
CC deamination of cytosine. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolyzes single-stranded DNA or mismatched double-stranded
CC DNA and polynucleotides, releasing free uracil.; EC=3.2.2.27;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily.
CC UNG family. {ECO:0000305}.
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DR EMBL; AL123456; CCP45781.1; -; Genomic_DNA.
DR PIR; E70672; E70672.
DR RefSeq; NP_217492.1; NC_000962.3.
DR RefSeq; WP_003899565.1; NZ_NVQJ01000015.1.
DR PDB; 2ZHX; X-ray; 3.10 A; A/C/E/G/I/K/M=1-227.
DR PDB; 3A7N; X-ray; 1.95 A; A=1-227.
DR PDB; 4WPK; X-ray; 0.98 A; A=1-227.
DR PDB; 4WPL; X-ray; 1.15 A; A=1-227.
DR PDB; 4WRU; X-ray; 1.24 A; A=1-227.
DR PDB; 4WRV; X-ray; 1.44 A; A=1-227.
DR PDB; 4WRW; X-ray; 1.90 A; A=1-227.
DR PDB; 4WRX; X-ray; 1.40 A; A=1-227.
DR PDB; 4WRY; X-ray; 1.43 A; A=1-227.
DR PDB; 4WRZ; X-ray; 1.19 A; A=1-227.
DR PDB; 4WS0; X-ray; 1.97 A; A=1-227.
DR PDB; 4WS1; X-ray; 1.40 A; A=1-227.
DR PDB; 4WS2; X-ray; 1.13 A; A=1-227.
DR PDB; 4WS3; X-ray; 1.40 A; A=1-227.
DR PDB; 4WS4; X-ray; 1.18 A; A=1-227.
DR PDB; 4WS5; X-ray; 1.40 A; A=1-227.
DR PDB; 4WS6; X-ray; 1.10 A; A=1-227.
DR PDB; 4WS7; X-ray; 1.88 A; A=1-227.
DR PDB; 4WS8; X-ray; 1.40 A; A=1-227.
DR PDBsum; 2ZHX; -.
DR PDBsum; 3A7N; -.
DR PDBsum; 4WPK; -.
DR PDBsum; 4WPL; -.
DR PDBsum; 4WRU; -.
DR PDBsum; 4WRV; -.
DR PDBsum; 4WRW; -.
DR PDBsum; 4WRX; -.
DR PDBsum; 4WRY; -.
DR PDBsum; 4WRZ; -.
DR PDBsum; 4WS0; -.
DR PDBsum; 4WS1; -.
DR PDBsum; 4WS2; -.
DR PDBsum; 4WS3; -.
DR PDBsum; 4WS4; -.
DR PDBsum; 4WS5; -.
DR PDBsum; 4WS6; -.
DR PDBsum; 4WS7; -.
DR PDBsum; 4WS8; -.
DR AlphaFoldDB; P9WFQ9; -.
DR SMR; P9WFQ9; -.
DR STRING; 83332.Rv2976c; -.
DR PaxDb; P9WFQ9; -.
DR DNASU; 887410; -.
DR GeneID; 45426965; -.
DR GeneID; 887410; -.
DR KEGG; mtu:Rv2976c; -.
DR TubercuList; Rv2976c; -.
DR eggNOG; COG0692; Bacteria.
DR OMA; WEAVTEQ; -.
DR PhylomeDB; P9WFQ9; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004844; F:uracil DNA N-glycosylase activity; IDA:MTBBASE.
DR GO; GO:0006284; P:base-excision repair; IDA:MTBBASE.
DR GO; GO:0097510; P:base-excision repair, AP site formation via deaminated base removal; IBA:GO_Central.
DR CDD; cd10027; UDG-F1-like; 1.
DR Gene3D; 3.40.470.10; -; 1.
DR HAMAP; MF_00148; UDG; 1.
DR InterPro; IPR002043; UDG_fam1.
DR InterPro; IPR018085; Ura-DNA_Glyclase_AS.
DR InterPro; IPR005122; Uracil-DNA_glycosylase-like.
DR InterPro; IPR036895; Uracil-DNA_glycosylase-like_sf.
DR PANTHER; PTHR11264; PTHR11264; 1.
DR Pfam; PF03167; UDG; 1.
DR SMART; SM00986; UDG; 1.
DR SUPFAM; SSF52141; SSF52141; 1.
DR TIGRFAMs; TIGR00628; ung; 1.
DR PROSITE; PS00130; U_DNA_GLYCOSYLASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA damage; DNA repair; Hydrolase;
KW Reference proteome.
FT CHAIN 1..227
FT /note="Uracil-DNA glycosylase"
FT /id="PRO_0000176119"
FT ACT_SITE 68
FT /note="Proton acceptor"
FT /evidence="ECO:0000250"
FT TURN 1..3
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 6..9
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 12..17
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 19..21
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 22..38
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 46..48
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 51..54
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 57..59
FT /evidence="ECO:0007829|PDB:4WPK"
FT STRAND 61..68
FT /evidence="ECO:0007829|PDB:4WPK"
FT TURN 73..75
FT /evidence="ECO:0007829|PDB:4WPK"
FT STRAND 78..81
FT /evidence="ECO:0007829|PDB:2ZHX"
FT HELIX 92..105
FT /evidence="ECO:0007829|PDB:4WPK"
FT STRAND 111..113
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 116..119
FT /evidence="ECO:0007829|PDB:4WPK"
FT TURN 120..122
FT /evidence="ECO:0007829|PDB:4WPK"
FT STRAND 123..129
FT /evidence="ECO:0007829|PDB:4WPK"
FT TURN 137..142
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 145..158
FT /evidence="ECO:0007829|PDB:4WPK"
FT STRAND 159..161
FT /evidence="ECO:0007829|PDB:4WPK"
FT STRAND 163..169
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 170..173
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 174..179
FT /evidence="ECO:0007829|PDB:4WPK"
FT TURN 181..183
FT /evidence="ECO:0007829|PDB:4WRX"
FT STRAND 184..189
FT /evidence="ECO:0007829|PDB:4WPK"
FT TURN 194..203
FT /evidence="ECO:0007829|PDB:4WPK"
FT HELIX 206..216
FT /evidence="ECO:0007829|PDB:4WPK"
SQ SEQUENCE 227 AA; 24481 MW; 379B5DB72894A304 CRC64;
MTARPLSELV ERGWAAALEP VADQVAHMGQ FLRAEIAAGR RYLPAGSNVL RAFTFPFDNV
RVLIVGQDPY PTPGHAVGLS FSVAPDVRPW PRSLANIFDE YTADLGYPLP SNGDLTPWAQ
RGVLLLNRVL TVRPSNPASH RGKGWEAVTE CAIRALAARA APLVAILWGR DASTLKPMLA
AGNCVAIESP HPSPLSASRG FFGSRPFSRA NELLVGMGAE PIDWRLP
