ID   UNG_RFVKA               Reviewed;         218 AA.
AC   P32941; Q77PB8;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   02-DEC-2020, entry version 85.
DE   RecName: Full=Uracil-DNA glycosylase;
DE            Short=UDG;
DE            EC=3.2.2.27;
GN   Name=UNG; ORFNames=D6R, s079R;
OS   Rabbit fibroma virus (strain Kasza) (RFV) (Shope fibroma virus (strain
OS   Kasza)).
OC   Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes;
OC   Chitovirales; Poxviridae; Chordopoxvirinae; Leporipoxvirus.
OX   NCBI_TaxID=10272;
OH   NCBI_TaxID=9986; Oryctolagus cuniculus (Rabbit).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION, AND FUNCTION.
RX   PubMed=8389453; DOI=10.1073/pnas.90.10.4518;
RA   Upton C., Stuart D.T., McFadden G.;
RT   "Identification of a poxvirus gene encoding a uracil-DNA glycosylase.";
RL   Proc. Natl. Acad. Sci. U.S.A. 90:4518-4522(1993).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1329373; DOI=10.1016/0168-1702(92)90104-h;
RA   Strayer D.S., Jerng H.H.;
RT   "Sequence and analysis of the BamHI 'D' fragment of Shope fibroma virus:
RT   comparison with similar regions of related poxviruses.";
RL   Virus Res. 25:117-132(1992).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1660196; DOI=10.1016/0042-6822(91)90529-k;
RA   Strayer D.S., Jerng H.H., O'Connor K.;
RT   "Sequence and analysis of a portion of the genomes of Shope fibroma virus
RT   and malignant rabbit fibroma virus that is important for viral replication
RT   in lymphocytes.";
RL   Virology 185:585-595(1991).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=10562495; DOI=10.1006/viro.1999.0002;
RA   Willer D.O., McFadden G., Evans D.H.;
RT   "The complete genome sequence of shope (Rabbit) fibroma virus.";
RL   Virology 264:319-343(1999).
RN   [5]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-10.
RX   PubMed=1649507; DOI=10.1016/0042-6822(91)91009-6;
RA   Upton C., Stuart D., McFadden G.;
RT   "Identification and DNA sequence of the large subunit of the capping enzyme
RT   from Shope fibroma virus.";
RL   Virology 183:773-777(1991).
CC   -!- FUNCTION: Excises uracil residues from the DNA which can arise as a
CC       result of misincorporation of dUMP residues by DNA polymerase or due to
CC       deamination of cytosine. Also part of a heterodimeric processivity
CC       factor which potentiates the DNA polymerase activity. Binds to DNA (By
CC       similarity). {ECO:0000250, ECO:0000269|PubMed:8389453}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolyzes single-stranded DNA or mismatched double-stranded
CC         DNA and polynucleotides, releasing free uracil.; EC=3.2.2.27;
CC   -!- SUBUNIT: Homodimer. Interacts with protein A20. Component of the
CC       Uracil-DNA glycosylase(UDG)-A20-polymerase complex; A20 and UDG form a
CC       heterodimeric processivity factor that associates with E9 to form the
CC       processive polymerase holoenzyme (By similarity). {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily.
CC       UNG family. {ECO:0000305}.
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DR   EMBL; L01417; AAA47229.1; -; Genomic_DNA.
DR   EMBL; AF170722; AAF17961.1; -; Genomic_DNA.
DR   RefSeq; NP_051968.1; NC_001266.1.
DR   SMR; P32941; -.
DR   GeneID; 1486922; -.
DR   KEGG; vg:1486922; -.
DR   Proteomes; UP000000868; Genome.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0016799; F:hydrolase activity, hydrolyzing N-glycosyl compounds; IEA:InterPro.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-KW.
DR   Gene3D; 3.40.470.10; -; 1.
DR   InterPro; IPR018085; Ura-DNA_Glyclase_AS.
DR   InterPro; IPR036895; Uracil-DNA_glycosylase-like_sf.
DR   SUPFAM; SSF52141; SSF52141; 1.
DR   PROSITE; PS00130; U_DNA_GLYCOSYLASE; 1.
PE   1: Evidence at protein level;
KW   DNA damage; DNA repair; DNA-binding; Hydrolase; Reference proteome.
FT   CHAIN           1..218
FT                   /note="Uracil-DNA glycosylase"
FT                   /id="PRO_0000176178"
FT   ACT_SITE        68
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU10072"
SQ   SEQUENCE   218 AA;  25569 MW;  7E16AFFB83F814DD CRC64;
     MRRVFLSHEP YVIEYHEDWE NIITRLVDMY NEVAEWILKD DTSPTPDKFF KQLSVSLKDK
     RVCVCGIDPY PRDATGVPFE SHNFTKKTIK YIAETVSNIT GVRYYKGYNL NNVEGVFPWN
     YYLSCKIGET KSHALHWKRI SKLLLQHITK YVNVLYCLGK TDFANIRSIL ETPVTTVIGY
     HPAAREKQFE KDKGFEIVNV LLEINDKPSI RWEQGFSY