UNG_STAAR
ID UNG_STAAR Reviewed; 218 AA.
AC Q6GJ88;
DT 21-DEC-2004, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2004, sequence version 1.
DT 25-MAY-2022, entry version 93.
DE RecName: Full=Uracil-DNA glycosylase {ECO:0000255|HAMAP-Rule:MF_00148};
DE Short=UDG {ECO:0000255|HAMAP-Rule:MF_00148};
DE EC=3.2.2.27 {ECO:0000255|HAMAP-Rule:MF_00148};
GN Name=ung {ECO:0000255|HAMAP-Rule:MF_00148}; OrderedLocusNames=SAR0586;
OS Staphylococcus aureus (strain MRSA252).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=282458;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MRSA252;
RX PubMed=15213324; DOI=10.1073/pnas.0402521101;
RA Holden M.T.G., Feil E.J., Lindsay J.A., Peacock S.J., Day N.P.J.,
RA Enright M.C., Foster T.J., Moore C.E., Hurst L., Atkin R., Barron A.,
RA Bason N., Bentley S.D., Chillingworth C., Chillingworth T., Churcher C.,
RA Clark L., Corton C., Cronin A., Doggett J., Dowd L., Feltwell T., Hance Z.,
RA Harris B., Hauser H., Holroyd S., Jagels K., James K.D., Lennard N.,
RA Line A., Mayes R., Moule S., Mungall K., Ormond D., Quail M.A.,
RA Rabbinowitsch E., Rutherford K.M., Sanders M., Sharp S., Simmonds M.,
RA Stevens K., Whitehead S., Barrell B.G., Spratt B.G., Parkhill J.;
RT "Complete genomes of two clinical Staphylococcus aureus strains: evidence
RT for the rapid evolution of virulence and drug resistance.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:9786-9791(2004).
CC -!- FUNCTION: Excises uracil residues from the DNA which can arise as a
CC result of misincorporation of dUMP residues by DNA polymerase or due to
CC deamination of cytosine. {ECO:0000255|HAMAP-Rule:MF_00148}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolyzes single-stranded DNA or mismatched double-stranded
CC DNA and polynucleotides, releasing free uracil.; EC=3.2.2.27;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00148};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00148}.
CC -!- SIMILARITY: Belongs to the uracil-DNA glycosylase (UDG) superfamily.
CC UNG family. {ECO:0000255|HAMAP-Rule:MF_00148}.
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DR EMBL; BX571856; CAG39606.1; -; Genomic_DNA.
DR RefSeq; WP_000455258.1; NC_002952.2.
DR PDB; 3WDF; X-ray; 1.48 A; A/B=1-218.
DR PDB; 3WDG; X-ray; 2.20 A; A=1-218.
DR PDBsum; 3WDF; -.
DR PDBsum; 3WDG; -.
DR AlphaFoldDB; Q6GJ88; -.
DR SMR; Q6GJ88; -.
DR KEGG; sar:SAR0586; -.
DR HOGENOM; CLU_032162_3_1_9; -.
DR OMA; PDNGYLM; -.
DR OrthoDB; 1260295at2; -.
DR Proteomes; UP000000596; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004844; F:uracil DNA N-glycosylase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006284; P:base-excision repair; IEA:UniProtKB-UniRule.
DR CDD; cd10027; UDG-F1-like; 1.
DR Gene3D; 3.40.470.10; -; 1.
DR HAMAP; MF_00148; UDG; 1.
DR InterPro; IPR002043; UDG_fam1.
DR InterPro; IPR018085; Ura-DNA_Glyclase_AS.
DR InterPro; IPR005122; Uracil-DNA_glycosylase-like.
DR InterPro; IPR036895; Uracil-DNA_glycosylase-like_sf.
DR PANTHER; PTHR11264; PTHR11264; 1.
DR Pfam; PF03167; UDG; 1.
DR SMART; SM00986; UDG; 1.
DR SUPFAM; SSF52141; SSF52141; 1.
DR TIGRFAMs; TIGR00628; ung; 1.
DR PROSITE; PS00130; U_DNA_GLYCOSYLASE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytoplasm; DNA damage; DNA repair; Hydrolase.
FT CHAIN 1..218
FT /note="Uracil-DNA glycosylase"
FT /id="PRO_0000176139"
FT ACT_SITE 59
FT /note="Proton acceptor"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00148"
FT HELIX 3..13
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 17..29
FT /evidence="ECO:0007829|PDB:3WDF"
FT STRAND 32..34
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 36..38
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 41..45
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 48..50
FT /evidence="ECO:0007829|PDB:3WDF"
FT STRAND 53..57
FT /evidence="ECO:0007829|PDB:3WDF"
FT TURN 64..66
FT /evidence="ECO:0007829|PDB:3WDF"
FT STRAND 68..70
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 82..94
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 105..110
FT /evidence="ECO:0007829|PDB:3WDF"
FT STRAND 112..118
FT /evidence="ECO:0007829|PDB:3WDF"
FT TURN 126..131
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 134..148
FT /evidence="ECO:0007829|PDB:3WDF"
FT STRAND 153..158
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 159..162
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 163..167
FT /evidence="ECO:0007829|PDB:3WDF"
FT TURN 170..172
FT /evidence="ECO:0007829|PDB:3WDF"
FT STRAND 173..178
FT /evidence="ECO:0007829|PDB:3WDF"
FT TURN 183..185
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 186..190
FT /evidence="ECO:0007829|PDB:3WDF"
FT HELIX 195..205
FT /evidence="ECO:0007829|PDB:3WDF"
SQ SEQUENCE 218 AA; 24967 MW; 5731B93297F0D154 CRC64;
MEWSQIFHDI TTKHDFKAMH DFLEKEYSTA IVYPDRENIY QAFDLTPFEN IKVVILGQDP
YHGPNQAHGL AFSVQPNAKF PPSLRNMYKE LADDIGCVRQ TPHLQDWARE GVLLLNTVLT
VRQGEANSHR DIGWETFTDE IIKAVSDYKE HVVFILWGKP AQQKIKLIDT SKHCIIKSVH
PSPLSAYRGF FGSKPYSKAN TYLESVGKSP INWCESEA
