UNK_DROME
ID UNK_DROME Reviewed; 599 AA.
AC Q86B79; A4V397; Q24580; Q5BIG8; Q960U9;
DT 29-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2003, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=RING finger protein unkempt;
GN Name=unk; ORFNames=CG4620;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAO41593.1};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RC TISSUE=Embryo {ECO:0000269|PubMed:1339381};
RX PubMed=1339381; DOI=10.1093/genetics/131.2.377;
RA Mohler J., Weiss N., Murli S., Mohammadi S., Vani K., Vasilakis G.,
RA Song C.H., Epstein A., Kuang T., English J., Cherdak D.;
RT "The embryonically active gene, unkempt, of Drosophila encodes a Cys3His
RT finger protein.";
RL Genetics 131:377-388(1992).
RN [2] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Rubin G.M., Celniker S.E.;
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 3-599.
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Embryo {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [6]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-411, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RX PubMed=17372656; DOI=10.1039/b617545g;
RA Bodenmiller B., Mueller L.N., Pedrioli P.G.A., Pflieger D., Juenger M.A.,
RA Eng J.K., Aebersold R., Tao W.A.;
RT "An integrated chemical, mass spectrometric and computational strategy for
RT (quantitative) phosphoproteomics: application to Drosophila melanogaster
RT Kc167 cells.";
RL Mol. Biosyst. 3:275-286(2007).
CC -!- FUNCTION: Essential for late larval/early pupal development.
CC {ECO:0000269|PubMed:1339381}.
CC -!- INTERACTION:
CC Q86B79; Q9N2M8: hdc; NbExp=3; IntAct=EBI-90517, EBI-97141;
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:1339381}.
CC -!- TISSUE SPECIFICITY: Ubiquitous in most somatic tissues from syncytial
CC embryo through to embryo stage 15. Expression becomes restricted
CC predominantly to the CNS at stages 16 and 17.
CC {ECO:0000269|PubMed:1339381}.
CC -!- DEVELOPMENTAL STAGE: Expressed both maternally and zygotically.
CC {ECO:0000269|PubMed:1339381}.
CC -!- DISRUPTION PHENOTYPE: Flies exhibit an 'unkempt' appearance: small
CC rough eyes, held out wings and crossed scutellar bristles.
CC {ECO:0000269|PubMed:1339381}.
CC -!- SIMILARITY: Belongs to the unkempt family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK93256.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=AAX33404.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=CAA77616.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; Z11527; CAA77616.1; ALT_INIT; mRNA.
DR EMBL; AE014297; AAO41593.1; -; Genomic_DNA.
DR EMBL; AE014297; AAO41594.1; -; Genomic_DNA.
DR EMBL; BT021256; AAX33404.1; ALT_INIT; mRNA.
DR EMBL; AY051832; AAK93256.1; ALT_INIT; mRNA.
DR PIR; S42526; S42526.
DR RefSeq; NP_001247253.1; NM_001260324.2.
DR RefSeq; NP_001247255.1; NM_001260326.2.
DR RefSeq; NP_788722.1; NM_176545.3.
DR RefSeq; NP_788723.1; NM_176546.3.
DR AlphaFoldDB; Q86B79; -.
DR SMR; Q86B79; -.
DR BioGRID; 67683; 8.
DR DIP; DIP-22281N; -.
DR IntAct; Q86B79; 35.
DR iPTMnet; Q86B79; -.
DR ABCD; Q86B79; 5 sequenced antibodies.
DR DNASU; 42738; -.
DR EnsemblMetazoa; FBtr0084402; FBpp0083794; FBgn0004395.
DR EnsemblMetazoa; FBtr0084403; FBpp0083795; FBgn0004395.
DR EnsemblMetazoa; FBtr0305571; FBpp0294022; FBgn0004395.
DR EnsemblMetazoa; FBtr0305573; FBpp0294024; FBgn0004395.
DR GeneID; 42738; -.
DR KEGG; dme:Dmel_CG4620; -.
DR UCSC; CG4620-RA; d. melanogaster.
DR CTD; 85451; -.
DR FlyBase; FBgn0004395; unk.
DR VEuPathDB; VectorBase:FBgn0004395; -.
DR GeneTree; ENSGT00940000169050; -.
DR InParanoid; Q86B79; -.
DR PhylomeDB; Q86B79; -.
DR Reactome; R-DME-983168; Antigen processing: Ubiquitination & Proteasome degradation.
DR SignaLink; Q86B79; -.
DR BioGRID-ORCS; 42738; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 42738; -.
DR PRO; PR:Q86B79; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0004395; Expressed in cleaving embryo and 23 other tissues.
DR ExpressionAtlas; Q86B79; baseline and differential.
DR Genevisible; Q86B79; DM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0003677; F:DNA binding; NAS:UniProtKB.
DR GO; GO:0008270; F:zinc ion binding; ISM:FlyBase.
DR GO; GO:0008407; P:chaeta morphogenesis; IMP:UniProtKB.
DR GO; GO:0048749; P:compound eye development; IMP:FlyBase.
DR GO; GO:0007476; P:imaginal disc-derived wing morphogenesis; IMP:UniProtKB.
DR GO; GO:0002164; P:larval development; IMP:UniProtKB.
DR GO; GO:0030182; P:neuron differentiation; IMP:FlyBase.
DR GO; GO:0050767; P:regulation of neurogenesis; IMP:FlyBase.
DR Gene3D; 3.30.40.10; -; 1.
DR InterPro; IPR045234; Unkempt-like.
DR InterPro; IPR040594; Unkempt_Znf.
DR InterPro; IPR000571; Znf_CCCH.
DR InterPro; IPR036855; Znf_CCCH_sf.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR14493; PTHR14493; 2.
DR Pfam; PF00642; zf-CCCH; 1.
DR Pfam; PF18384; zf_CCCH_5; 1.
DR SMART; SM00356; ZnF_C3H1; 4.
DR SUPFAM; SSF90229; SSF90229; 1.
DR PROSITE; PS50103; ZF_C3H1; 5.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Developmental protein; DNA-binding; Metal-binding;
KW Phosphoprotein; Reference proteome; Repeat; Zinc; Zinc-finger.
FT CHAIN 1..599
FT /note="RING finger protein unkempt"
FT /id="PRO_0000056300"
FT ZN_FING 71..100
FT /note="C3H1-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 111..141
FT /note="C3H1-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 194..220
FT /note="C3H1-type 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 230..264
FT /note="C3H1-type 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 272..300
FT /note="C3H1-type 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00723"
FT ZN_FING 556..591
FT /note="RING-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00175"
FT MOD_RES 411
FT /note="Phosphoserine"
FT /evidence="ECO:0000269|PubMed:17372656"
FT CONFLICT 411
FT /note="S -> R (in Ref. 4; AAX33404)"
FT /evidence="ECO:0000305"
FT CONFLICT 558
FT /note="K -> E (in Ref. 4; AAX33404)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 599 AA; 68442 MW; 4BCBD702FF6C9846 CRC64;
MLANETNKLL LSSQQEKPNH YTYLKEFRVE QCQSFLQHKC NQHRPFVCFN WHFQNQRRRR
PVRKRDGTFN YSADNYCTKY DETTGICPEG DECPYLHRTA GDTERRYHLR YYKTCMCVHD
TDSRGYCVKN GLHCAFAHGM QDQRPPVYDI KELETLQNAE STLDSTNALN ALDKERNLMN
EDPKWQDTNY VLANYKTEPC KRPPRLCRQG YACPQYHNSK DKRRSPRKYK YRSTPCPNVK
HGEEWGEPGN CEAGDNCQYC HTRTEQQFHP EIYKSTKCND VQQAGYCPRS VFCAFAHVEP
CSMDDPRENS LSASLANTSL LTRSSAPINI PNTTLSNSIN DFNSGSFAVN IPSSSLTYSP
TNHANLFNVD AFNYGGSNKL SNSLSATQND SSLFFPSRII SPGFGDGLSI SPSVRISELN
TIRDDINSSS VGNSLFENTL NTAKNAFSLQ SLQSQNNSDL GRITNELLTK NAQIHKLNGR
FEDMACKLKI AELHRDKAKQ EAQEWKERYD LAQIQLNLPA ELRDLSIQKL KQLQSKLRTD
LEEVDKVLYL ENAKKCMKCE ENNRTVTLEP CNHLSICNTC AESVTECPYC QVPVITTHT
