UNPG_DROME
ID UNPG_DROME Reviewed; 485 AA.
AC Q4V5A3; Q24136;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Homeobox protein unplugged;
GN Name=unpg {ECO:0000312|EMBL:AAY55169.1, ECO:0000312|FlyBase:FBgn0015561};
GN Synonyms=unp {ECO:0000303|PubMed:8582298}; ORFNames=CG1650;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000305, ECO:0000312|EMBL:AAA97404.1}
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX PubMed=8582298; DOI=10.1242/dev.121.11.3901;
RA Chiang C., Young K.E., Beachy P.A.;
RT "Control of Drosophila tracheal branching by the novel homeodomain gene
RT unplugged, a regulatory target for genes of the bithorax complex.";
RL Development 121:3901-3912(1995).
RN [2] {ECO:0000312|EMBL:AAF58968.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3] {ECO:0000305, ECO:0000312|EMBL:AAF58968.1}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4] {ECO:0000312|EMBL:AAY55169.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (MAY-2005) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, AND TISSUE SPECIFICITY.
RX PubMed=12702651; DOI=10.1242/dev.00438;
RA Hirth F., Kammermeier L., Frei E., Walldorf U., Noll M., Reichert H.;
RT "An urbilaterian origin of the tripartite brain: developmental genetic
RT insights from Drosophila.";
RL Development 130:2365-2373(2003).
CC -!- FUNCTION: Plays a regulatory role in neural branching of the tracheae:
CC segment-specific aspects of these neural branching patterns appear to
CC be generated by homeotic regulation of expression. May have a role with
CC oc/otd in the postembryonic development of the brain.
CC {ECO:0000269|PubMed:12702651, ECO:0000269|PubMed:8582298}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC ECO:0000269|PubMed:8582298}. Note=In tracheal precursor cells as they
CC migrate and extend. {ECO:0000269|PubMed:8582298}.
CC -!- TISSUE SPECIFICITY: Expressed in the neuroectodermal and mesectodermal
CC cells at the ventral midline of stage 8 embryos, Subsequently,
CC expression domains in the CNS widen and have their most anterior border
CC in the posterior deutocerebrum. Oc/otd and unpg are mutual repressors
CC at the interface of their brain-specific expression domains. Expression
CC fades during germ band retraction and is then restricted to subset of
CC cells by stage 14. Expressed in the founder cells of the cerebral
CC branch within the first tracheal metamere. Outside the CNS, expression
CC is seen in two clusters of ectodermal cells located laterally within
CC the labial and first thoracic segments of stage 9 embryos. By stage 13,
CC the expression is detected in a few cells close to the dorsal midline
CC of the embryos. {ECO:0000269|PubMed:12702651,
CC ECO:0000269|PubMed:8582298}.
CC -!- DEVELOPMENTAL STAGE: First detected in 4 hours embryos, expression
CC persists throughout embryogenesis, levels drop during larval stages and
CC then increase again during pupal and adult stages.
CC {ECO:0000269|PubMed:8582298}.
CC -!- DISRUPTION PHENOTYPE: Embryos display segmentally repeated ganglionic
CC branches that stall and fail to penetrate the CNS and the segment-
CC specific cerebral branch and associated cerebral anastomosis fail to
CC form. {ECO:0000269|PubMed:8582298}.
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DR EMBL; U35427; AAA97404.1; -; mRNA.
DR EMBL; AE013599; AAF58968.1; -; Genomic_DNA.
DR EMBL; BT022753; AAY55169.1; -; mRNA.
DR RefSeq; NP_477146.1; NM_057798.3.
DR AlphaFoldDB; Q4V5A3; -.
DR SMR; Q4V5A3; -.
DR BioGRID; 61780; 5.
DR IntAct; Q4V5A3; 5.
DR STRING; 7227.FBpp0087667; -.
DR PaxDb; Q4V5A3; -.
DR PRIDE; Q4V5A3; -.
DR DNASU; 35942; -.
DR EnsemblMetazoa; FBtr0088586; FBpp0087667; FBgn0015561.
DR GeneID; 35942; -.
DR KEGG; dme:Dmel_CG1650; -.
DR UCSC; CG1650-RA; d. melanogaster.
DR CTD; 35942; -.
DR FlyBase; FBgn0015561; unpg.
DR VEuPathDB; VectorBase:FBgn0015561; -.
DR eggNOG; KOG0489; Eukaryota.
DR GeneTree; ENSGT00940000154365; -.
DR HOGENOM; CLU_571441_0_0_1; -.
DR InParanoid; Q4V5A3; -.
DR OMA; MSPRNYN; -.
DR OrthoDB; 858478at2759; -.
DR PhylomeDB; Q4V5A3; -.
DR BioGRID-ORCS; 35942; 0 hits in 3 CRISPR screens.
DR GenomeRNAi; 35942; -.
DR PRO; PR:Q4V5A3; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0015561; Expressed in lateral cord glial cell (Drosophila) and 17 other tissues.
DR Genevisible; Q4V5A3; DM.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IMP:UniProtKB.
DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR GO; GO:0007420; P:brain development; IMP:UniProtKB.
DR GO; GO:0051960; P:regulation of nervous system development; IMP:UniProtKB.
DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR GO; GO:0006355; P:regulation of transcription, DNA-templated; IMP:UniProtKB.
DR CDD; cd00086; homeodomain; 1.
DR InterPro; IPR042982; GBX-1/2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017970; Homeobox_CS.
DR InterPro; IPR001356; Homeobox_dom.
DR InterPro; IPR020479; Homeobox_metazoa.
DR InterPro; IPR031253; Unplugged.
DR PANTHER; PTHR24334; PTHR24334; 1.
DR PANTHER; PTHR24334:SF0; PTHR24334:SF0; 1.
DR Pfam; PF00046; Homeodomain; 1.
DR PRINTS; PR00024; HOMEOBOX.
DR SMART; SM00389; HOX; 1.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS00027; HOMEOBOX_1; 1.
DR PROSITE; PS50071; HOMEOBOX_2; 1.
PE 2: Evidence at transcript level;
KW DNA-binding; Homeobox; Nucleus; Reference proteome; Transcription;
KW Transcription regulation.
FT CHAIN 1..485
FT /note="Homeobox protein unplugged"
FT /id="PRO_0000299499"
FT DNA_BIND 319..378
FT /note="Homeobox"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT REGION 1..65
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 114..157
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 212..325
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 125..143
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 213..233
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 255..284
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 307..321
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 107
FT /note="G -> A (in Ref. 1; AAA97404)"
FT /evidence="ECO:0000305"
FT CONFLICT 481
FT /note="A -> P (in Ref. 1; AAA97404)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 485 AA; 52230 MW; 16B4EF888ADE1D56 CRC64;
MERPALLQNG EIGTMESPTT RLASKPFPKP FSIESLIANQ TPATATPPSP PEERDQEQEA
EQEQELSARA MVASSALGLT QFPLYNPWLH GYFAQNHERL THLIAGGCYL PSSPAGHPAA
QQPQAQAQPQ PPPPHPPTHA LEKQLPPTLP HPLDTRFLPF NPAAAGVAPT DLSYRRLAEL
MNQDYVHSLS VHARLQHMAA AGRMHEDQAN PGMAQLQEPT PPQAHSSPAK SGSHSPMEPA
LDVGMDEDFE CSGDSCSDIS LTMSPRNYNG EMDKSRNGAY TNSDSEDCSD DEGAQSRHEG
GGMGGKDSQG NGSSSNSKSR RRRTAFTSEQ LLELEREFHA KKYLSLTERS QIATSLKLSE
VQVKIWFQNR RAKWKRVKAG LTSHGLGRNG TTSGTKIVVP IPVHVNRFAV RSQHQQLEKM
CLSGPKPDLR KKLSAEAIGG FEKFSGSTNA SSPSGGPVGL GVGVGVGVGV GLGVSTPLSL
ARSIY
