ID   CA225_CATRO             Reviewed;         538 AA.
AC   W8JWW3;
DT   10-APR-2019, integrated into UniProtKB/Swiss-Prot.
DT   14-MAY-2014, sequence version 1.
DT   03-AUG-2022, entry version 18.
DE   RecName: Full=Cytochrome P450 72A225 {ECO:0000303|PubMed:24710322};
DE            Short=CrCYP72A225 {ECO:0000303|PubMed:24710322};
DE            EC=1.14.-.- {ECO:0000305};
GN   Name=CYP72A225 {ECO:0000303|PubMed:24710322};
GN   ORFNames=Caros003930 {ECO:0000305};
OS   Catharanthus roseus (Madagascar periwinkle) (Vinca rosea).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Gentianales; Apocynaceae; Rauvolfioideae; Vinceae;
OC   Catharanthinae; Catharanthus.
OX   NCBI_TaxID=4058;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=cv. Little Bright Eyes;
RX   PubMed=24710322; DOI=10.1038/ncomms4606;
RA   Miettinen K., Dong L., Navrot N., Schneider T., Burlat V., Pollier J.,
RA   Woittiez L., van der Krol S., Lugan R., Ilc T., Verpoorte R.,
RA   Oksman-Caldentey K.M., Martinoia E., Bouwmeester H., Goossens A.,
RA   Memelink J., Werck-Reichhart D.;
RT   "The seco-iridoid pathway from Catharanthus roseus.";
RL   Nat. Commun. 5:3606-3606(2014).
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC   -!- WEB RESOURCE: Name=ORCAE database;
CC       URL="https://bioinformatics.psb.ugent.be/orcae/overview/Catro";
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DR   EMBL; KF302074; AHK60841.1; -; mRNA.
DR   AlphaFoldDB; W8JWW3; -.
DR   SMR; W8JWW3; -.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0020037; F:heme binding; IEA:InterPro.
DR   GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR   GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR   GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR   Gene3D; 1.10.630.10; -; 1.
DR   InterPro; IPR001128; Cyt_P450.
DR   InterPro; IPR017972; Cyt_P450_CS.
DR   InterPro; IPR002401; Cyt_P450_E_grp-I.
DR   InterPro; IPR036396; Cyt_P450_sf.
DR   Pfam; PF00067; p450; 1.
DR   PRINTS; PR00463; EP450I.
DR   PRINTS; PR00385; P450.
DR   SUPFAM; SSF48264; SSF48264; 1.
DR   PROSITE; PS00086; CYTOCHROME_P450; 1.
PE   2: Evidence at transcript level;
KW   Glycoprotein; Heme; Iron; Membrane; Metal-binding; Monooxygenase;
KW   Oxidoreductase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..538
FT                   /note="Cytochrome P450 72A225"
FT                   /id="PRO_0000446413"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        344..364
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   BINDING         486
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /ligand_part="Fe"
FT                   /ligand_part_id="ChEBI:CHEBI:18248"
FT                   /note="axial binding residue"
FT                   /evidence="ECO:0000250|UniProtKB:P04798"
FT   CARBOHYD        88
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        137
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
FT   CARBOHYD        325
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00498"
SQ   SEQUENCE   538 AA;  61937 MW;  340868948644790C CRC64;
     MISTCHSWHM FQIRNREMDV FYRGISVLGT CFFLLLAWKM LNWAWIKPKK LEKRLRQLGL
     KGNSYRLLIG DLGETSQMDK EAKSKPINFT NHIIPRIMPF LHKIIQSHGK NPFIWQGPNP
     AVIITDPELI KEVLTKNYTY QKPPSDDLGK LISQGVAAYD KDKWAKHRRL LNPAFHIEKL
     KHMLPAFHLS CSEMLKKWEK LASASAEGSF ELDVWPYLQD MTSDVISRTA FGSNLEEGKN
     IFKLQKEILE HILQILQSFY FPGMLYLPTK RIRRMKKISK EVHSSVLNII MKRVEAMKAG
     EPSNDDLLGI LLDSNFKEIE NDNGNNSNKN CGMSFEEVIE ECKLFYFAGQ ETTAVLLVWT
     LLLLSKHPEW QMRARDEVLQ IFGNSEPDFD ALNHLKVITM VINEVLRLYP PGFMMSRVIH
     EDTKLGSLSL PGGISIFLPT IMLHHDEELW GDDAKEFKPE RFSEGISKAT KGQFIYFPFS
     GGPRICIGNN FAMLEAKMAL ALILQRFTFE ISPSYTHAPH TLVTLQPQYG AQLILYKR