UP12_ORYSI
ID UP12_ORYSI Reviewed; 283 AA.
AC P83643; A2YST8; B8BC84;
DT 20-MAR-2007, integrated into UniProtKB/Swiss-Prot.
DT 29-APR-2008, sequence version 2.
DT 25-MAY-2022, entry version 60.
DE RecName: Full=ACT domain-containing protein DS12, chloroplastic {ECO:0000305};
DE AltName: Full=Uncharacterized protein DS12 from 2D-PAGE of leaf;
DE Flags: Precursor;
GN ORFNames=OsI_027381, OsI_28383 {ECO:0000312|EMBL:EEC83162.1};
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Guang-Lu-Ai No.4;
RX PubMed=17522955; DOI=10.1007/s11103-007-9174-7;
RA Liu X., Lu T., Yu S., Li Y., Huang Y., Huang T., Zhang L., Zhu J., Zhao Q.,
RA Fan D., Mu J., Shangguan Y., Feng Q., Guan J., Ying K., Zhang Y., Lin Z.,
RA Sun Z., Qian Q., Lu Y., Han B.;
RT "A collection of 10,096 indica rice full-length cDNAs reveals highly
RT expressed sequence divergence between Oryza sativa indica and japonica
RT subspecies.";
RL Plant Mol. Biol. 65:403-415(2007).
RN [3] {ECO:0000305}
RP PROTEIN SEQUENCE OF 207-215 AND 234-245, AND INDUCTION.
RC STRAIN=cv. IR62266-42-6-2; TISSUE=Leaf {ECO:0000269|PubMed:12362332};
RX PubMed=12362332;
RX DOI=10.1002/1615-9861(200209)2:9<1131::aid-prot1131>3.0.co;2-1;
RA Salekdeh G.H., Siopongco J., Wade L.J., Ghareyazie B., Bennett J.;
RT "Proteomic analysis of rice leaves during drought stress and recovery.";
RL Proteomics 2:1131-1145(2002).
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast {ECO:0000305}.
CC -!- INDUCTION: Repressed by drought stress. {ECO:0000269|PubMed:12362332}.
CC -!- MISCELLANEOUS: On the 2D-gel the determined pI of this unknown protein
CC is: 4.7, its MW is: 36 kDa. {ECO:0000269|PubMed:12362332}.
CC -!- SEQUENCE CAUTION:
CC Sequence=EEC83162.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; CM000133; EEC83162.1; ALT_INIT; Genomic_DNA.
DR EMBL; CT858998; -; NOT_ANNOTATED_CDS; mRNA.
DR AlphaFoldDB; P83643; -.
DR STRING; 39946.P83643; -.
DR PRIDE; P83643; -.
DR HOGENOM; CLU_066532_1_0_1; -.
DR Proteomes; UP000007015; Chromosome 8.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR InterPro; IPR040217; ACR1-12.
DR InterPro; IPR045865; ACT-like_dom_sf.
DR PANTHER; PTHR31096; PTHR31096; 1.
DR SUPFAM; SSF55021; SSF55021; 1.
PE 1: Evidence at protein level;
KW Chloroplast; Direct protein sequencing; Plastid; Reference proteome;
KW Repeat; Transit peptide.
FT TRANSIT 1..56
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 57..283
FT /note="ACT domain-containing protein DS12, chloroplastic"
FT /id="PRO_0000280231"
FT DOMAIN 91..171
FT /note="ACT 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
FT DOMAIN 206..276
FT /note="ACT 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01007"
FT REGION 14..78
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..78
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 283 AA; 30323 MW; AEE558E937C4F7A4 CRC64;
MAEMAVTAAL RPCSGVSPAV SGTSHRRRRP AAWRALAPPP PHAGLRLSSP AVRVPRAASS
AAVEDGSSSN TDTVPTPKVI IDQDSDPDAT IVEITLGDRL GDLLDTMNAL KNLGLNVVKA
SVCLDSTGKH IKLAITKLST GRKIGEPELL EAVRLTIINN MIQYHPEASS QLALGATFGP
EPPTELVDVD IATHIDIYDD GPDRSLLVVE TADRPGLLVD LVKIIDDINI TVQSGEFDTE
GLLAKAKFHV SYRGKPLIKA LQQVLANSLR YFLRRPTTEE GSY