UPK1B_HUMAN
ID UPK1B_HUMAN Reviewed; 260 AA.
AC O75841; O60753; Q9UIM2; Q9UNX6;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 30-NOV-2010, sequence version 5.
DT 03-AUG-2022, entry version 165.
DE RecName: Full=Uroplakin-1b;
DE Short=UP1b;
DE AltName: Full=Tetraspanin-20;
DE Short=Tspan-20;
DE AltName: Full=Uroplakin Ib;
DE Short=UPIb;
GN Name=UPK1B; Synonyms=TSPAN20;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ARG-113.
RC TISSUE=Urinary bladder urothelium;
RX PubMed=9818021; DOI=10.1111/j.1349-7006.1998.tb00643.x;
RA Yuasa T., Yoshiki T., Tanaka T., Kim C.J., Isono T., Okada Y.;
RT "Expression of uroplakin Ib and uroplakin III genes in tissues and
RT peripheral blood of patients with transitional cell carcinoma.";
RL Jpn. J. Cancer Res. 89:879-882(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND VARIANT ARG-113.
RC TISSUE=Urothelium;
RX PubMed=9935153;
RX DOI=10.1002/(sici)1097-0215(19990209)80:4<533::aid-ijc9>3.0.co;2-5;
RA Finch J.L., Miller J., Aspinall J.O., Cowled P.A.;
RT "Cloning of the human uroplakin 1B cDNA and analysis of its expression in
RT urothelial-tumor cell lines and bladder-carcinoma tissue.";
RL Int. J. Cancer 80:533-538(1999).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Corneal epithelium;
RA Adachi W., Nishida K., Kinoshita S., Matsubara K., Okubo K.;
RT "Molecular cloning of human uroplakin Ib cDNA.";
RL Submitted (MAR-1997) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16641997; DOI=10.1038/nature04728;
RA Muzny D.M., Scherer S.E., Kaul R., Wang J., Yu J., Sudbrak R., Buhay C.J.,
RA Chen R., Cree A., Ding Y., Dugan-Rocha S., Gill R., Gunaratne P.,
RA Harris R.A., Hawes A.C., Hernandez J., Hodgson A.V., Hume J., Jackson A.,
RA Khan Z.M., Kovar-Smith C., Lewis L.R., Lozado R.J., Metzker M.L.,
RA Milosavljevic A., Miner G.R., Morgan M.B., Nazareth L.V., Scott G.,
RA Sodergren E., Song X.-Z., Steffen D., Wei S., Wheeler D.A., Wright M.W.,
RA Worley K.C., Yuan Y., Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M.,
RA Brown M.J., Chen G., Chen Z., Clendenning J., Clerc-Blankenburg K.P.,
RA Chen R., Chen Z., Davis C., Delgado O., Dinh H.H., Dong W., Draper H.,
RA Ernst S., Fu G., Gonzalez-Garay M.L., Garcia D.K., Gillett W., Gu J.,
RA Hao B., Haugen E., Havlak P., He X., Hennig S., Hu S., Huang W.,
RA Jackson L.R., Jacob L.S., Kelly S.H., Kube M., Levy R., Li Z., Liu B.,
RA Liu J., Liu W., Lu J., Maheshwari M., Nguyen B.-V., Okwuonu G.O.,
RA Palmeiri A., Pasternak S., Perez L.M., Phelps K.A., Plopper F.J., Qiang B.,
RA Raymond C., Rodriguez R., Saenphimmachak C., Santibanez J., Shen H.,
RA Shen Y., Subramanian S., Tabor P.E., Verduzco D., Waldron L., Wang J.,
RA Wang J., Wang Q., Williams G.A., Wong G.K.-S., Yao Z., Zhang J., Zhang X.,
RA Zhao G., Zhou J., Zhou Y., Nelson D., Lehrach H., Reinhardt R.,
RA Naylor S.L., Yang H., Olson M., Weinstock G., Gibbs R.A.;
RT "The DNA sequence, annotation and analysis of human chromosome 3.";
RL Nature 440:1194-1198(2006).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT ARG-113.
RC TISSUE=Ovary;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 11-252, AND VARIANT ARG-113.
RX PubMed=9846985; DOI=10.1016/s0002-9440(10)65709-4;
RA Lobban E.D., Smith B.A., Hall G.D., Harnden P., Roberts P., Selby P.J.,
RA Trejdosiewicz L.K., Southgate J.;
RT "Uroplakin gene expression by normal and neoplastic human urothelium.";
RL Am. J. Pathol. 153:1957-1967(1998).
RN [7]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 103-142, AND VARIANT ARG-113.
RX PubMed=9073521; DOI=10.1006/geno.1996.4589;
RA Finch J.L., Webb G.C., Evdokiou A., Cowled P.A.;
RT "Chromosomal localization of the human urothelial 'tetraspan' gene, UPK1B,
RT to 3q13.3-q21 and detection of a TaqI polymorphism.";
RL Genomics 40:501-503(1997).
CC -!- FUNCTION: Component of the asymmetric unit membrane (AUM); a highly
CC specialized biomembrane elaborated by terminally differentiated
CC urothelial cells. May play an important role in normal bladder
CC epithelial physiology, possibly in regulating membrane permeability of
CC superficial umbrella cells or in stabilizing the apical membrane
CC through AUM/cytoskeletal interactions (By similarity). {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer with uroplakin-3A (UPK3A) or uroplakin-3B (UPK3B).
CC {ECO:0000250}.
CC -!- INTERACTION:
CC O75841; Q8NFU1: BEST2; NbExp=3; IntAct=EBI-12237619, EBI-19947314;
CC O75841; P19397: CD53; NbExp=3; IntAct=EBI-12237619, EBI-6657396;
CC O75841; Q07108: CD69; NbExp=3; IntAct=EBI-12237619, EBI-2836595;
CC O75841; Q9HA82: CERS4; NbExp=3; IntAct=EBI-12237619, EBI-2622997;
CC O75841; O95471: CLDN7; NbExp=3; IntAct=EBI-12237619, EBI-740744;
CC O75841; Q9Y4D2: DAGLA; NbExp=6; IntAct=EBI-12237619, EBI-12808806;
CC O75841; Q9UNE0-2: EDAR; NbExp=3; IntAct=EBI-12237619, EBI-12964110;
CC O75841; Q5JX71: FAM209A; NbExp=3; IntAct=EBI-12237619, EBI-18304435;
CC O75841; O15552: FFAR2; NbExp=3; IntAct=EBI-12237619, EBI-2833872;
CC O75841; Q9Y680: FKBP7; NbExp=3; IntAct=EBI-12237619, EBI-3918971;
CC O75841; Q8TBE3: FNDC9; NbExp=3; IntAct=EBI-12237619, EBI-12142257;
CC O75841; P48165: GJA8; NbExp=3; IntAct=EBI-12237619, EBI-17458373;
CC O75841; P08034: GJB1; NbExp=3; IntAct=EBI-12237619, EBI-17565645;
CC O75841; Q8N6U8: GPR161; NbExp=3; IntAct=EBI-12237619, EBI-6255622;
CC O75841; O60883: GPR37L1; NbExp=3; IntAct=EBI-12237619, EBI-2927498;
CC O75841; Q8TED1: GPX8; NbExp=3; IntAct=EBI-12237619, EBI-11721746;
CC O75841; Q6UXK2: ISLR2; NbExp=3; IntAct=EBI-12237619, EBI-1266923;
CC O75841; P05107: ITGB2; NbExp=3; IntAct=EBI-12237619, EBI-300173;
CC O75841; Q8N6L0: KASH5; NbExp=3; IntAct=EBI-12237619, EBI-749265;
CC O75841; P48051: KCNJ6; NbExp=3; IntAct=EBI-12237619, EBI-12017638;
CC O75841; P43628: KIR2DL3; NbExp=3; IntAct=EBI-12237619, EBI-8632435;
CC O75841; P26715: KLRC1; NbExp=3; IntAct=EBI-12237619, EBI-9018187;
CC O75841; Q9GZY8-5: MFF; NbExp=3; IntAct=EBI-12237619, EBI-11956541;
CC O75841; Q96JQ5: MS4A4A; NbExp=3; IntAct=EBI-12237619, EBI-12820341;
CC O75841; O14524-2: NEMP1; NbExp=3; IntAct=EBI-12237619, EBI-10969203;
CC O75841; Q13113: PDZK1IP1; NbExp=3; IntAct=EBI-12237619, EBI-716063;
CC O75841; Q96FE7: PIK3IP1; NbExp=3; IntAct=EBI-12237619, EBI-10285708;
CC O75841; Q5VZY2: PLPP4; NbExp=3; IntAct=EBI-12237619, EBI-10485931;
CC O75841; Q9UBD6: RHCG; NbExp=3; IntAct=EBI-12237619, EBI-15853497;
CC O75841; Q9NR31: SAR1A; NbExp=3; IntAct=EBI-12237619, EBI-3920694;
CC O75841; Q5VUM1: SDHAF4; NbExp=3; IntAct=EBI-12237619, EBI-16769525;
CC O75841; Q96DD7: SHISA4; NbExp=3; IntAct=EBI-12237619, EBI-18035902;
CC O75841; Q9Y3P8: SIT1; NbExp=3; IntAct=EBI-12237619, EBI-6977215;
CC O75841; Q12908: SLC10A2; NbExp=3; IntAct=EBI-12237619, EBI-18114847;
CC O75841; Q9NQQ7-3: SLC35C2; NbExp=3; IntAct=EBI-12237619, EBI-17295964;
CC O75841; Q9NP94: SLC39A2; NbExp=3; IntAct=EBI-12237619, EBI-12898013;
CC O75841; O43278-2: SPINT1; NbExp=3; IntAct=EBI-12237619, EBI-12078338;
CC O75841; Q8WWF3: SSMEM1; NbExp=3; IntAct=EBI-12237619, EBI-17280858;
CC O75841; Q16623: STX1A; NbExp=3; IntAct=EBI-12237619, EBI-712466;
CC O75841; Q86SS6: SYT9; NbExp=3; IntAct=EBI-12237619, EBI-19129467;
CC O75841; Q8IV31: TMEM139; NbExp=3; IntAct=EBI-12237619, EBI-7238458;
CC O75841; Q96Q45-2: TMEM237; NbExp=3; IntAct=EBI-12237619, EBI-10982110;
CC O75841; E9PQX1: TMEM262; NbExp=3; IntAct=EBI-12237619, EBI-17180389;
CC O75841; Q96B21: TMEM45B; NbExp=3; IntAct=EBI-12237619, EBI-3923061;
CC O75841; Q969K7: TMEM54; NbExp=3; IntAct=EBI-12237619, EBI-3922833;
CC O75841; Q6ZNR0-2: TMEM91; NbExp=3; IntAct=EBI-12237619, EBI-13345425;
CC O75841; Q71RG4: TMUB2; NbExp=3; IntAct=EBI-12237619, EBI-2820477;
CC O75841; Q86XK7: VSIG1; NbExp=3; IntAct=EBI-12237619, EBI-18323486;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Bladder epithelium.
CC -!- PTM: N-glycosylated with high-mannose oligosaccharides. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR EMBL; AB015234; BAA33724.1; -; mRNA.
DR EMBL; AF042331; AAC77794.1; -; mRNA.
DR EMBL; AB002155; BAA88878.1; -; mRNA.
DR EMBL; AC083800; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC063568; AAH63568.1; -; mRNA.
DR EMBL; AF082888; AAD09401.1; -; mRNA.
DR EMBL; AF067147; AAC17446.1; -; Genomic_DNA.
DR CCDS; CCDS2985.1; -.
DR RefSeq; NP_008883.2; NM_006952.3.
DR AlphaFoldDB; O75841; -.
DR SMR; O75841; -.
DR BioGRID; 113195; 56.
DR IntAct; O75841; 50.
DR STRING; 9606.ENSP00000264234; -.
DR TCDB; 8.A.40.7.2; the tetraspanin (tetraspanin) family.
DR iPTMnet; O75841; -.
DR PhosphoSitePlus; O75841; -.
DR BioMuta; UPK1B; -.
DR jPOST; O75841; -.
DR MassIVE; O75841; -.
DR MaxQB; O75841; -.
DR PaxDb; O75841; -.
DR PeptideAtlas; O75841; -.
DR PRIDE; O75841; -.
DR ProteomicsDB; 50224; -.
DR Antibodypedia; 32685; 240 antibodies from 28 providers.
DR DNASU; 7348; -.
DR Ensembl; ENST00000264234.8; ENSP00000264234.3; ENSG00000114638.8.
DR GeneID; 7348; -.
DR KEGG; hsa:7348; -.
DR MANE-Select; ENST00000264234.8; ENSP00000264234.3; NM_006952.4; NP_008883.2.
DR UCSC; uc003ecc.4; human.
DR CTD; 7348; -.
DR DisGeNET; 7348; -.
DR GeneCards; UPK1B; -.
DR HGNC; HGNC:12578; UPK1B.
DR HPA; ENSG00000114638; Tissue enhanced (stomach, urinary bladder).
DR MIM; 602380; gene.
DR neXtProt; NX_O75841; -.
DR OpenTargets; ENSG00000114638; -.
DR PharmGKB; PA37210; -.
DR VEuPathDB; HostDB:ENSG00000114638; -.
DR eggNOG; KOG3882; Eukaryota.
DR GeneTree; ENSGT00940000160779; -.
DR InParanoid; O75841; -.
DR OMA; QDHCCGV; -.
DR OrthoDB; 944403at2759; -.
DR PhylomeDB; O75841; -.
DR TreeFam; TF335659; -.
DR PathwayCommons; O75841; -.
DR SignaLink; O75841; -.
DR BioGRID-ORCS; 7348; 8 hits in 1055 CRISPR screens.
DR ChiTaRS; UPK1B; human.
DR GeneWiki; UPK1B; -.
DR GenomeRNAi; 7348; -.
DR Pharos; O75841; Tbio.
DR PRO; PR:O75841; -.
DR Proteomes; UP000005640; Chromosome 3.
DR RNAct; O75841; protein.
DR Bgee; ENSG00000114638; Expressed in palpebral conjunctiva and 109 other tissues.
DR ExpressionAtlas; O75841; baseline and differential.
DR Genevisible; O75841; HS.
DR GO; GO:0120001; C:apical plasma membrane urothelial plaque; IEA:Ensembl.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; NAS:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005198; F:structural molecule activity; NAS:UniProtKB.
DR GO; GO:0030855; P:epithelial cell differentiation; IDA:UniProtKB.
DR GO; GO:0009617; P:response to bacterium; IEA:Ensembl.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR InterPro; IPR034766; Uroplakin-1b.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR PANTHER; PTHR19282:SF24; PTHR19282:SF24; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..260
FT /note="Uroplakin-1b"
FT /id="PRO_0000219289"
FT TOPO_DOM 1..15
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 16..36
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 37..60
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..86
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 87..107
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 108..229
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 230..250
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 251..260
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT VARIANT 113
FT /note="Q -> R (in dbSNP:rs9840317)"
FT /evidence="ECO:0000269|PubMed:15489334,
FT ECO:0000269|PubMed:9073521, ECO:0000269|PubMed:9818021,
FT ECO:0000269|PubMed:9846985, ECO:0000269|PubMed:9935153"
FT /id="VAR_028780"
FT CONFLICT 137
FT /note="N -> D (in Ref. 1; BAA33724)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 260 AA; 29643 MW; 0512AF8D33884C0F CRC64;
MAKDNSTVRC FQGLLIFGNV IIGCCGIALT AECIFFVSDQ HSLYPLLEAT DNDDIYGAAW
IGIFVGICLF CLSVLGIVGI MKSSRKILLA YFILMFIVYA FEVASCITAA TQQDFFTPNL
FLKQMLERYQ NNSPPNNDDQ WKNNGVTKTW DRLMLQDNCC GVNGPSDWQK YTSAFRTENN
DADYPWPRQC CVMNNLKEPL NLEACKLGVP GFYHNQGCYE LISGPMNRHA WGVAWFGFAI
LCWTFWVLLG TMFYWSRIEY
