UPK3A_MOUSE
ID UPK3A_MOUSE Reviewed; 287 AA.
AC Q9JKX8; Q27QV3;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 26-MAY-2009, sequence version 3.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Uroplakin-3a;
DE Short=UP3a;
DE AltName: Full=Uroplakin III;
DE Short=UPIII;
DE Flags: Precursor;
GN Name=Upk3a; Synonyms=Upk3;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ICR; TISSUE=Urinary bladder;
RA Kwon D.-N., Hwang K.-C., Chu H.-J.;
RT "Cloning of mouse uroplakin III cDNA.";
RL Submitted (DEC-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=Swiss Webster; TISSUE=Urinary bladder;
RX PubMed=16814572; DOI=10.1016/j.ympev.2006.04.023;
RA Garcia-Espana A., Chung P.-J., Zhao X., Lee A., Pellicer A., Yu J.,
RA Sun T.-T., Desalle R.;
RT "Origin of the tetraspanin uroplakins and their co-evolution with
RT associated proteins: implications for uroplakin structure and function.";
RL Mol. Phylogenet. Evol. 41:355-367(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the asymmetric unit membrane (AUM); a highly
CC specialized biomembrane elaborated by terminally differentiated
CC urothelial cells. May play an important role in AUM-cytoskeleton
CC interaction in terminally differentiated urothelial cells. It also
CC contributes to the formation of urothelial glycocalyx which may play an
CC important role in preventing bacterial adherence (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer with uroplakin-1B (UPK1B). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Single-pass type I membrane protein {ECO:0000250}. Note=Heterodimer
CC formation with UPK1B is a prerequisite to exit out of the endoplasmic
CC reticulum (ER). {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the uroplakin-3 family. {ECO:0000305}.
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DR EMBL; AF222750; AAF34681.2; -; mRNA.
DR EMBL; DQ387454; ABD52002.1; -; mRNA.
DR EMBL; CH466550; EDL04442.1; -; Genomic_DNA.
DR EMBL; BC125336; AAI25337.1; -; mRNA.
DR EMBL; BC125338; AAI25339.1; -; mRNA.
DR CCDS; CCDS27716.1; -.
DR RefSeq; NP_075967.2; NM_023478.2.
DR AlphaFoldDB; Q9JKX8; -.
DR IntAct; Q9JKX8; 3.
DR MINT; Q9JKX8; -.
DR STRING; 10090.ENSMUSP00000023070; -.
DR GlyGen; Q9JKX8; 3 sites.
DR iPTMnet; Q9JKX8; -.
DR PhosphoSitePlus; Q9JKX8; -.
DR PaxDb; Q9JKX8; -.
DR PRIDE; Q9JKX8; -.
DR ProteomicsDB; 297871; -.
DR Antibodypedia; 13681; 267 antibodies from 26 providers.
DR DNASU; 22270; -.
DR Ensembl; ENSMUST00000023070; ENSMUSP00000023070; ENSMUSG00000022435.
DR GeneID; 22270; -.
DR KEGG; mmu:22270; -.
DR UCSC; uc007xcs.2; mouse.
DR CTD; 7380; -.
DR MGI; MGI:98914; Upk3a.
DR VEuPathDB; HostDB:ENSMUSG00000022435; -.
DR eggNOG; ENOG502S14V; Eukaryota.
DR GeneTree; ENSGT00940000153392; -.
DR HOGENOM; CLU_082608_1_0_1; -.
DR InParanoid; Q9JKX8; -.
DR OMA; EKPFCVF; -.
DR OrthoDB; 1269506at2759; -.
DR PhylomeDB; Q9JKX8; -.
DR TreeFam; TF336628; -.
DR BioGRID-ORCS; 22270; 1 hit in 74 CRISPR screens.
DR PRO; PR:Q9JKX8; -.
DR Proteomes; UP000000589; Chromosome 15.
DR RNAct; Q9JKX8; protein.
DR Bgee; ENSMUSG00000022435; Expressed in urinary bladder urothelium and 53 other tissues.
DR Genevisible; Q9JKX8; MM.
DR GO; GO:0016324; C:apical plasma membrane; IDA:MGI.
DR GO; GO:0120001; C:apical plasma membrane urothelial plaque; IDA:MGI.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0000902; P:cell morphogenesis; IMP:MGI.
DR GO; GO:0030855; P:epithelial cell differentiation; ISO:MGI.
DR GO; GO:0001822; P:kidney development; IMP:MGI.
DR GO; GO:0055075; P:potassium ion homeostasis; IMP:MGI.
DR GO; GO:0055078; P:sodium ion homeostasis; IMP:MGI.
DR GO; GO:0015840; P:urea transport; IMP:MGI.
DR GO; GO:0060157; P:urinary bladder development; IMP:MGI.
DR GO; GO:0006833; P:water transport; IMP:MGI.
DR InterPro; IPR024831; Uroplakin-3.
DR InterPro; IPR024825; Uroplakin-3a.
DR PANTHER; PTHR15446; PTHR15446; 1.
DR PANTHER; PTHR15446:SF17; PTHR15446:SF17; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..18
FT /evidence="ECO:0000250"
FT CHAIN 19..287
FT /note="Uroplakin-3a"
FT /id="PRO_0000022638"
FT TOPO_DOM 19..207
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 208..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..287
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 243..287
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 243..269
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 170
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 2..3
FT /note="LL -> PP (in Ref. 1; AAF34681)"
FT /evidence="ECO:0000305"
FT CONFLICT 187
FT /note="W -> R (in Ref. 1; AAF34681)"
FT /evidence="ECO:0000305"
FT CONFLICT 195
FT /note="S -> L (in Ref. 1; AAF34681)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 287 AA; 30990 MW; 44CF39DA6CA30E88 CRC64;
MLLLWALLAL GCLRCGWTVN LQPQLASVTF ATNNPTLTTV ALEKPLCMFD SSEPLSGSYE
VYLYAMVDSA MSRNVSVQDS AGVPLSTTFR QTQGGRSGPY KAAAFDLTPC GDLPSLDAVG
DVTQASEILN AYLVRVGNNG TCFWDPNFQG LCNPPLTAAT EYRFKYVLVN MSTGLVQDQT
LWSDPIWTNR PIPYSAIDTW PGRRSGGMIV ITSILGSLPF FLLVGFAGAI ILSFVDMGSS
DGEMTHDSQI TQEAVPKTLG TSEPSYSSVN RGPPLDRAEV FSSKLQD
