UPK3B_HUMAN
ID UPK3B_HUMAN Reviewed; 320 AA.
AC Q9BT76; A6NHH5; A8K231; A8MZA8; B3KPU5; Q75MM5; Q86W06;
DT 19-SEP-2003, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 2.
DT 03-AUG-2022, entry version 150.
DE RecName: Full=Uroplakin-3b;
DE Short=UP3b;
DE AltName: Full=Uroplakin IIIb;
DE Short=UPIIIb;
DE AltName: Full=p35;
DE Flags: Precursor;
GN Name=UPK3B;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), INTERACTION WITH UPK1B, SUBCELLULAR
RP LOCATION, AND VARIANT ARG-319.
RX PubMed=12446744; DOI=10.1083/jcb.200204102;
RA Deng F.-M., Liang F.-X., Tu L., Resing K.A., Hu P., Supino M., Hu C.-C.A.,
RA Zhou G., Ding M., Kreibich G., Sun T.-T.;
RT "Uroplakin IIIb, a urothelial differentiation marker, dimerizes with
RT uroplakin Ib as an early step of urothelial plaque assembly.";
RL J. Cell Biol. 159:685-694(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2), AND VARIANT
RP ARG-319.
RC TISSUE=Placenta, and Subthalamic nucleus;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12853948; DOI=10.1038/nature01782;
RA Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H.,
RA Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K.,
RA Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A.,
RA Delehaunty K.D., Miner T.L., Nash W.E., Cordes M., Du H., Sun H.,
RA Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A.,
RA Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P.,
RA Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M.,
RA Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S.,
RA Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R.,
RA Strowmatt C., Latreille P., Miller N., Johnson D., Murray J.,
RA Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W.,
RA Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E.,
RA Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A.,
RA Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E.,
RA Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E.,
RA Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A.,
RA Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A.,
RA Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R.,
RA McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H.,
RA Wilson R.K.;
RT "The DNA sequence of human chromosome 7.";
RL Nature 424:157-164(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT ARG-319.
RC TISSUE=Uterus;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Component of the asymmetric unit membrane (AUM); a highly
CC specialized biomembrane elaborated by terminally differentiated
CC urothelial cells. May play an important role in AUM-cytoskeleton
CC interaction in terminally differentiated urothelial cells. It also
CC contributes to the formation of urothelial glycocalyx which may play an
CC important role in preventing bacterial adherence (By similarity).
CC {ECO:0000250}.
CC -!- SUBUNIT: Heterodimer with uroplakin-1B (UPK1B).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Single-pass type I
CC membrane protein {ECO:0000305}. Note=Heterodimer formation with UPK1B
CC is a prerequisite to exit out of the endoplasmic reticulum (ER).
CC {ECO:0000269|PubMed:12446744}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q9BT76-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9BT76-2; Sequence=VSP_037327;
CC Name=3;
CC IsoId=Q9BT76-3; Sequence=VSP_037327, VSP_054295;
CC -!- SIMILARITY: Belongs to the uroplakin-3 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAO89507.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAS07526.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AY233462; AAO89507.1; ALT_FRAME; mRNA.
DR EMBL; AK056760; BAG51807.1; -; mRNA.
DR EMBL; AK290096; BAF82785.1; -; mRNA.
DR EMBL; AC004980; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC006972; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC007003; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC114737; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC007078; AAS07526.1; ALT_SEQ; Genomic_DNA.
DR EMBL; BC004304; AAH04304.1; -; mRNA.
DR CCDS; CCDS87512.1; -. [Q9BT76-3]
DR RefSeq; NP_001334613.1; NM_001347684.1. [Q9BT76-3]
DR RefSeq; NP_085047.1; NM_030570.3.
DR RefSeq; NP_872624.1; NM_182683.2.
DR RefSeq; NP_872625.1; NM_182684.2.
DR RefSeq; XP_005250669.1; XM_005250612.3.
DR RefSeq; XP_006716203.1; XM_006716140.3.
DR RefSeq; XP_006716204.1; XM_006716141.3.
DR AlphaFoldDB; Q9BT76; -.
DR BioGRID; 123295; 13.
DR IntAct; Q9BT76; 3.
DR STRING; 9606.ENSP00000257632; -.
DR TCDB; 8.A.90.2.2; the uroplakin 2/3 (upk2/3) family.
DR GlyGen; Q9BT76; 1 site.
DR iPTMnet; Q9BT76; -.
DR PhosphoSitePlus; Q9BT76; -.
DR BioMuta; UPK3B; -.
DR MassIVE; Q9BT76; -.
DR PaxDb; Q9BT76; -.
DR PeptideAtlas; Q9BT76; -.
DR PRIDE; Q9BT76; -.
DR ProteomicsDB; 1200; -.
DR ProteomicsDB; 78956; -. [Q9BT76-1]
DR ProteomicsDB; 78957; -. [Q9BT76-2]
DR Antibodypedia; 34816; 108 antibodies from 21 providers.
DR DNASU; 80761; -.
DR Ensembl; ENST00000257632.9; ENSP00000257632.5; ENSG00000243566.7. [Q9BT76-1]
DR Ensembl; ENST00000334348.8; ENSP00000334938.3; ENSG00000243566.7. [Q9BT76-3]
DR Ensembl; ENST00000394849.1; ENSP00000378319.1; ENSG00000243566.7. [Q9BT76-2]
DR Ensembl; ENST00000448265.7; ENSP00000441284.2; ENSG00000276184.4.
DR Ensembl; ENST00000615976.2; ENSP00000479568.1; ENSG00000276184.4.
DR GeneID; 105375355; -.
DR KEGG; hsa:105375355; -.
DR MANE-Select; ENST00000334348.8; ENSP00000334938.3; NM_001347684.2; NP_001334613.1. [Q9BT76-3]
DR UCSC; uc033ayx.2; human. [Q9BT76-1]
DR CTD; 105375355; -.
DR DisGeNET; 105375355; -.
DR GeneCards; UPK3B; -.
DR HGNC; HGNC:21444; UPK3B.
DR HPA; ENSG00000243566; Group enriched (adipose tissue, esophagus, lung, urinary bladder).
DR MIM; 611887; gene.
DR neXtProt; NX_Q9BT76; -.
DR OpenTargets; ENSG00000243566; -.
DR PharmGKB; PA134952181; -.
DR VEuPathDB; HostDB:ENSG00000243566; -.
DR eggNOG; ENOG502RZJD; Eukaryota.
DR GeneTree; ENSGT00940000153392; -.
DR HOGENOM; CLU_082608_0_0_1; -.
DR InParanoid; Q9BT76; -.
DR OMA; CEPGQDP; -.
DR OrthoDB; 1269506at2759; -.
DR PhylomeDB; Q9BT76; -.
DR TreeFam; TF336628; -.
DR PathwayCommons; Q9BT76; -.
DR SignaLink; Q9BT76; -.
DR BioGRID-ORCS; 105375355; 14 hits in 1000 CRISPR screens.
DR GenomeRNAi; 105375355; -.
DR Pharos; Q9BT76; Tbio.
DR PRO; PR:Q9BT76; -.
DR Proteomes; UP000005640; Chromosome 7.
DR RNAct; Q9BT76; protein.
DR Bgee; ENSG00000243566; Expressed in lower esophagus mucosa and 89 other tissues.
DR Genevisible; Q9BT76; HS.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0010629; P:negative regulation of gene expression; IMP:UniProtKB.
DR InterPro; IPR024831; Uroplakin-3.
DR InterPro; IPR024828; Uroplakin-3b.
DR PANTHER; PTHR15446; PTHR15446; 1.
DR PANTHER; PTHR15446:SF15; PTHR15446:SF15; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT SIGNAL 1..29
FT /evidence="ECO:0000255"
FT CHAIN 30..320
FT /note="Uroplakin-3b"
FT /id="PRO_0000022640"
FT TOPO_DOM 30..240
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 241..266
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 267..320
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REGION 273..303
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 133
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 29..83
FT /note="Missing (in isoform 2 and isoform 3)"
FT /evidence="ECO:0000303|PubMed:12446744,
FT ECO:0000303|PubMed:14702039"
FT /id="VSP_037327"
FT VAR_SEQ 210..320
FT /note="EDPRIHRHLARAAKWQHDRHYLHPLFSGRPPTLGLLGSLYHALLQPVVAGGG
FT PGAAADRLLHGQALHDPPHPTQRGRHTAGGLQAWPGPPPQPQPLAWPLCMGLGEMGRWE
FT -> VKFLLMDTRGSPRAETKWSDPITLHQGKTPGSIDTWPGRRSGSMIVITSILSSLAG
FT LLLLAFLAASTMRFSSLWWPEEAPEQLRIGSFMGKRYMTHHIPPREAATLPVGCKPGLD
FT PLPSLSP (in isoform 3)"
FT /evidence="ECO:0000305"
FT /id="VSP_054295"
FT VARIANT 293
FT /note="Q -> R (in dbSNP:rs1636632)"
FT /id="VAR_034561"
FT VARIANT 319
FT /note="W -> R (in dbSNP:rs1799126)"
FT /evidence="ECO:0000269|PubMed:12446744,
FT ECO:0000269|PubMed:14702039, ECO:0000269|PubMed:15489334"
FT /id="VAR_047805"
SQ SEQUENCE 320 AA; 33882 MW; 64A1FE268A8BB0EB CRC64;
MGLPWGQPHL GLQMLLLALN CLRPSLSLGE WGSWMDASSQ TQGAGGPAGV IGPWAPAPLR
LGEAAPGTPT PVSVAHLLSP VATELVPYTP QITAWDLEGK VTATTFSLEQ PRCVFDGLAS
ASDTVWLVVA FSNASRGFQN PETLADIPAS PQLLTDGHYM TLPLSPDQLP CGDPMAGSGG
APVLRVGHDH GCHQQPFCNA PLPGPGPYRE DPRIHRHLAR AAKWQHDRHY LHPLFSGRPP
TLGLLGSLYH ALLQPVVAGG GPGAAADRLL HGQALHDPPH PTQRGRHTAG GLQAWPGPPP
QPQPLAWPLC MGLGEMGRWE
