UPL6_ARATH
ID UPL6_ARATH Reviewed; 1029 AA.
AC Q8RWB8; Q9LUV3;
DT 04-DEC-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=E3 ubiquitin-protein ligase UPL6;
DE Short=Ubiquitin-protein ligase 6;
DE EC=2.3.2.26;
DE AltName: Full=HECT-type E3 ubiquitin transferase UPL6;
GN Name=UPL6; OrderedLocusNames=At3g17205; ORFNames=MCE21.4, MGD8.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10907853; DOI=10.1093/dnares/7.3.217;
RA Kaneko T., Katoh T., Sato S., Nakamura Y., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. II. Sequence
RT features of the 4,251,695 bp regions covered by 90 P1, TAC and BAC
RT clones.";
RL DNA Res. 7:217-221(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA Cheuk R.F., Chen H., Kim C.J., Shinn P., Carninci P., Hayashizaki Y.,
RA Ishida J., Kamiya A., Kawai J., Narusaka M., Sakurai T., Satou M., Seki M.,
RA Shinozaki K., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Probable E3 ubiquitin-protein ligase which mediates
CC ubiquitination and subsequent proteasomal degradation of target
CC proteins. {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26;
CC -!- PATHWAY: Protein modification; protein ubiquitination.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According to EST
CC sequences.;
CC Name=1;
CC IsoId=Q8RWB8-1; Sequence=Displayed;
CC -!- SIMILARITY: Belongs to the UPL family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB02722.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AB022216; BAB02722.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP000384; BAB02722.1; JOINED; Genomic_DNA.
DR EMBL; CP002686; AEE75918.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE75920.1; -; Genomic_DNA.
DR EMBL; AY093202; AAM13201.1; -; mRNA.
DR EMBL; BT010593; AAQ89615.1; -; mRNA.
DR RefSeq; NP_001189915.1; NM_001202986.1. [Q8RWB8-1]
DR RefSeq; NP_188346.2; NM_112597.4. [Q8RWB8-1]
DR AlphaFoldDB; Q8RWB8; -.
DR SMR; Q8RWB8; -.
DR BioGRID; 6312; 3.
DR IntAct; Q8RWB8; 3.
DR STRING; 3702.AT3G17205.1; -.
DR iPTMnet; Q8RWB8; -.
DR PaxDb; Q8RWB8; -.
DR ProteomicsDB; 245281; -. [Q8RWB8-1]
DR EnsemblPlants; AT3G17205.1; AT3G17205.1; AT3G17205. [Q8RWB8-1]
DR EnsemblPlants; AT3G17205.3; AT3G17205.3; AT3G17205. [Q8RWB8-1]
DR GeneID; 820979; -.
DR Gramene; AT3G17205.1; AT3G17205.1; AT3G17205. [Q8RWB8-1]
DR Gramene; AT3G17205.3; AT3G17205.3; AT3G17205. [Q8RWB8-1]
DR KEGG; ath:AT3G17205; -.
DR Araport; AT3G17205; -.
DR TAIR; locus:2087939; AT3G17205.
DR eggNOG; KOG0942; Eukaryota.
DR InParanoid; Q8RWB8; -.
DR OMA; KRCNENQ; -.
DR OrthoDB; 1163565at2759; -.
DR PhylomeDB; Q8RWB8; -.
DR UniPathway; UPA00143; -.
DR PRO; PR:Q8RWB8; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8RWB8; baseline and differential.
DR GO; GO:0061630; F:ubiquitin protein ligase activity; IBA:GO_Central.
DR GO; GO:0000209; P:protein polyubiquitination; IBA:GO_Central.
DR GO; GO:0006511; P:ubiquitin-dependent protein catabolic process; IBA:GO_Central.
DR CDD; cd00078; HECTc; 1.
DR InterPro; IPR044611; E3B/C.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR000048; IQ_motif_EF-hand-BS.
DR PANTHER; PTHR45700; PTHR45700; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; SSF56204; 1.
DR PROSITE; PS50237; HECT; 1.
DR PROSITE; PS50096; IQ; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Reference proteome; Transferase;
KW Ubl conjugation pathway.
FT CHAIN 1..1029
FT /note="E3 ubiquitin-protein ligase UPL6"
FT /id="PRO_0000312024"
FT DOMAIN 45..74
FT /note="IQ"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00116"
FT DOMAIN 688..1029
FT /note="HECT"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
FT REGION 1..20
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 997
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 1029 AA; 117662 MW; AADC5A2E10E4A2EB CRC64;
MFFSGDPSTR KRVDLGGRST KERDARKLLE QTRMERNRRL LQKQQNSAAL KIQKFFRGRR
SMAIERSKVR HDFCETYGNN CQNVDRHCFE PGSSFLRQFL FFFKAQNSGD FVILVETCRL
LQSFVDSSGD IVSLFSGLDY SSEHNLVDFR VKKLAFTCIE AIHQNRNRLR DQLLVTPEEA
SISTAILMEA VSLLLDPKLP WVCKIVSYLQ KRKVFKLVRE MVTTAKESPR GKTMTGNILS
LERVLILIVP HIGREPCCCT VVDPRWSFSS MILTIPLIWK LFPNLKVVFA NPSLSQHYIH
QMASCIQKDT CVLPMETSPE FPGYACLLGN TLDTANVVLS QPECSLDMAI DIALVATFFL
ETLPPVKSSE GESRQGSSDE DDMLIDDVPG LVLNKALEQQ ITNAIDSRFL LQLTNVLFRQ
VSLGMQSYDE DKEALAIGTA SSFLYAAFNT LPLERIMTIL AYRTELVAVL WNYMKRCHEN
QKWSSMPKLL AYLPGDAPGW LLPLVVFCPV YKHMLMIVDN EEFYEREKPL SLQDIRLLII
ILKQALWQLL WVNPLTQPNT GKSVSNDLSK KNPIELIQNR MGIVVSELLS QLQDWNNRKQ
FTSSSDFQAD SVNEYFISQA IMEGTRANYI LMQAPFLIPF TSRVKIFTTQ LATARQSHGS
QGIFARNRFR IRRDHILEDA YNQMSALSED DLRSSIRVTF VNELGVEEAG IDGGGIFKDF
MEKITRAAFD VQYGLFKETA DHMLYPNPGS GMIHEQHLQF FHFLGSLLAK AMFEGILVDI
PFATFFLSKL KQKYNYLNDL PSLDPELYRH LIFLKRYKGD ISDLELYFVI LNNEYGERTE
EELLPGGQDM RVTNENVITF IHLVSNHRLN FQIRQQSSHF LRGFQQLIPK EWIDMFNEHE
LQVLISGSVD SLDIDDLRNN TNYAGGYHAG HYVIDMFWEV MKSFSTENQK KFLKFVTGCS
RGPLLGFKYL EPAFCIQRAA GSASNESVDR LPTSATCMNL LKLPPYQSKE LLETKLMYAI
SAEAGFDLS
