CAAT2_ARATH
ID CAAT2_ARATH Reviewed; 635 AA.
AC Q9ASS7; Q8H182;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 134.
DE RecName: Full=Cationic amino acid transporter 2, vacuolar;
GN Name=CAT2; OrderedLocusNames=At1g58030; ORFNames=T15M6;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RA de los Reyes C., Quan R., Chen H., Bautista V., Kim C.J., Ecker J.R.;
RT "Arabidopsis ORF clones.";
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15377779; DOI=10.1104/pp.104.045278;
RA Su Y.-H., Frommer W.B., Ludewig U.;
RT "Molecular and functional characterization of a family of amino acid
RT transporters from Arabidopsis.";
RL Plant Physiol. 136:3104-3113(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=17151019; DOI=10.1074/mcp.m600250-mcp200;
RA Jaquinod M., Villiers F., Kieffer-Jaquinod S., Hugouvieux V., Bruley C.,
RA Garin J., Bourguignon J.;
RT "A proteomics dissection of Arabidopsis thaliana vacuoles isolated from
RT cell culture.";
RL Mol. Cell. Proteomics 6:394-412(2007).
CC -!- FUNCTION: Permease involved in the transport of the cationic amino
CC acids. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:15377779,
CC ECO:0000269|PubMed:17151019}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15377779, ECO:0000269|PubMed:17151019}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, flowers, leaves, and
CC siliques. {ECO:0000269|PubMed:15377779}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.
CC {ECO:0000305}.
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DR EMBL; AC079604; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CP002684; AEE33488.1; -; Genomic_DNA.
DR EMBL; AF367304; AAK32891.1; -; mRNA.
DR EMBL; BT000457; AAN17434.1; -; mRNA.
DR EMBL; BT046174; ACI49773.1; -; mRNA.
DR RefSeq; NP_849822.1; NM_179491.3.
DR AlphaFoldDB; Q9ASS7; -.
DR SMR; Q9ASS7; -.
DR STRING; 3702.AT1G58030.1; -.
DR TCDB; 2.A.3.3.13; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; Q9ASS7; -.
DR PRIDE; Q9ASS7; -.
DR ProteomicsDB; 239122; -.
DR EnsemblPlants; AT1G58030.1; AT1G58030.1; AT1G58030.
DR GeneID; 842170; -.
DR Gramene; AT1G58030.1; AT1G58030.1; AT1G58030.
DR KEGG; ath:AT1G58030; -.
DR Araport; AT1G58030; -.
DR TAIR; locus:2196245; AT1G58030.
DR eggNOG; KOG1286; Eukaryota.
DR HOGENOM; CLU_007946_15_1_1; -.
DR InParanoid; Q9ASS7; -.
DR OMA; WFAKTHP; -.
DR OrthoDB; 439017at2759; -.
DR PhylomeDB; Q9ASS7; -.
DR PRO; PR:Q9ASS7; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9ASS7; baseline and differential.
DR Genevisible; Q9ASS7; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0080144; P:amino acid homeostasis; IMP:TAIR.
DR GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR029485; CAT_C.
DR Pfam; PF13520; AA_permease_2; 1.
DR Pfam; PF13906; AA_permease_C; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..635
FT /note="Cationic amino acid transporter 2, vacuolar"
FT /id="PRO_0000415778"
FT TOPO_DOM 1..48
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 49..69
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 70..76
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 77..97
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 98..108
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..131
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 132..171
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 172..192
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 193..200
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 201..221
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 222..235
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..280
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 281..301
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 302..324
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 325..345
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 346..376
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 377..397
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 398
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..419
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 420..493
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 494..514
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 515..524
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 525..545
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 546..560
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 561..581
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 582..585
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 586..606
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 607..635
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 391
FT /note="F -> I (in Ref. 3; AAN17434)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 635 AA; 67115 MW; 34F3D17A57DF738E CRC64;
MGFLVDTQKE GGGHSWGYVR SLVRRKQVDS ANGQSHGHQL ARALTVPHLV AIGVGATIGA
GVYILVGTVA REHSGPSLAL SFLIAGIAAG LSAFCYAELS SRCPSAGSAY HYSYICVGEG
VAWIIGWALI LEYTIGGSAV ARGISPNLAL IFGGEDGLPA ILARHQIPGL DIVVDPCAAI
LVFVVTGLLC MGIKESTFAQ GIVTAVNVCV LLFVIVAGSY LGFKTGWPGY ELPTGFFPFG
VDGMFAGSAT VFFAFIGFDS VASTAEEVRN PQRDLPIGIG LALLLCCSLY MMVSIVIVGL
IPYYAMDPDT PISSAFASHD MQWAVYLITL GAVMALCSAL MGALLPQPRI LMAMARDGLL
PSIFSDINKR TQVPVKATVA TGLCAATLAF FMDVSQLAGM VSVGTLLAFT MVAISVLILR
YVPPDEQPLP SSLQERIDSV SFICGETTSS GHVGTSDSSH QPLIVNNDAL VDVPLIKNQE
ALGCLVLSEE TRRIVAGWSI MFTCVGAFLL SYAASSLSFP GLIRYPLCGV GGCLLLAGLI
ALSSIDQDDA RHTFGHSGGY MCPFVPLLPI ICILINMYLL VNLGSATWAR VSVWLLIGVI
VYVFYGRKNS SLANAVYVTT AHAEEIYREH EGSLA
