CAAT4_ARATH
ID CAAT4_ARATH Reviewed; 600 AA.
AC Q8W4K3; F4J170; Q9SRU9;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Cationic amino acid transporter 4, vacuolar;
GN Name=CAT4; OrderedLocusNames=At3g03720; ORFNames=F20H23.25;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP TISSUE SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15377779; DOI=10.1104/pp.104.045278;
RA Su Y.-H., Frommer W.B., Ludewig U.;
RT "Molecular and functional characterization of a family of amino acid
RT transporters from Arabidopsis.";
RL Plant Physiol. 136:3104-3113(2004).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND SUBCELLULAR LOCATION [LARGE SCALE
RP ANALYSIS].
RX PubMed=17151019; DOI=10.1074/mcp.m600250-mcp200;
RA Jaquinod M., Villiers F., Kieffer-Jaquinod S., Hugouvieux V., Bruley C.,
RA Garin J., Bourguignon J.;
RT "A proteomics dissection of Arabidopsis thaliana vacuoles isolated from
RT cell culture.";
RL Mol. Cell. Proteomics 6:394-412(2007).
CC -!- FUNCTION: Permease involved in the transport of the cationic amino
CC acids. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Vacuole membrane {ECO:0000269|PubMed:17151019};
CC Multi-pass membrane protein {ECO:0000269|PubMed:17151019}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, flowers, and leaves.
CC {ECO:0000269|PubMed:15377779}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF00645.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AC009540; AAF00645.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002686; AEE73975.2; -; Genomic_DNA.
DR EMBL; AY062508; AAL32586.1; -; mRNA.
DR EMBL; BT008369; AAP37728.1; -; mRNA.
DR RefSeq; NP_001154586.1; NM_001161114.2.
DR AlphaFoldDB; Q8W4K3; -.
DR SMR; Q8W4K3; -.
DR BioGRID; 6509; 2.
DR IntAct; Q8W4K3; 2.
DR STRING; 3702.AT3G03720.1; -.
DR TCDB; 2.A.3.3.14; the amino acid-polyamine-organocation (apc) family.
DR iPTMnet; Q8W4K3; -.
DR PaxDb; Q8W4K3; -.
DR PRIDE; Q8W4K3; -.
DR ProteomicsDB; 240537; -.
DR EnsemblPlants; AT3G03720.1; AT3G03720.1; AT3G03720.
DR GeneID; 821176; -.
DR Gramene; AT3G03720.1; AT3G03720.1; AT3G03720.
DR KEGG; ath:AT3G03720; -.
DR Araport; AT3G03720; -.
DR TAIR; locus:2079414; AT3G03720.
DR eggNOG; KOG1286; Eukaryota.
DR HOGENOM; CLU_007946_15_1_1; -.
DR InParanoid; Q8W4K3; -.
DR OMA; VGTWQVP; -.
DR OrthoDB; 439017at2759; -.
DR PhylomeDB; Q8W4K3; -.
DR PRO; PR:Q8W4K3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q8W4K3; baseline and differential.
DR Genevisible; Q8W4K3; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0009705; C:plant-type vacuole membrane; IDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR029485; CAT_C.
DR Pfam; PF13520; AA_permease_2; 1.
DR Pfam; PF13906; AA_permease_C; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix; Transport; Vacuole.
FT CHAIN 1..600
FT /note="Cationic amino acid transporter 4, vacuolar"
FT /id="PRO_0000415780"
FT TOPO_DOM 1..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 54..60
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 82..92
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..115
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 116..152
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 153..173
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 174..184
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 185..205
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 206..220
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 221..241
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 242..264
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 265..285
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 286..308
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 309..329
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 330..360
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 361..381
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 382
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 383..403
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 404..462
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 463..483
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 484..492
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 493..513
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 514..528
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 529..549
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 550
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 551..571
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 572..600
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 600 AA; 63639 MW; B74D371C3067D882 CRC64;
MNSLVRRKQV DSVHLIKNDG PHQLAKKLSA VDLVAIGVGT TIGAGVYILV GTVAREHTGP
ALAVSFFIAG VAAALSACCY AELASRCPSA GSAYHYAYIC LGEGIAWLVG WALVLDYTIG
GSAIARGITP NLASFFGGLD NLPVFLARQT IPGVGIVVDP CAALLIMIVT ILLCFGIKES
STVQAIVTSV NVCTLVFIIV VGGYLACKTG WVGYDLPSGY FPFGLNGILA GSAVVFFSYI
GFDTVTSTAE EVKNPQRDLP LGIGIALLIC CILYMLLSVV IVGLVPYYSL NPDTPISSAF
GDSGMQWAAY ILTTGAITAL CASLLGSLLA QPRIFMAMAR DGLLPAFFSE ISPRTQVPVK
STIAIGVLAA ALAFFMDVAQ LSEMVSVGTL MAFTAVAVCV LVLRYVPPDG VPLSSSSQTL
SDTDESRAET ENFLVDAIES SDSPLLGNET ARDEKYFGKR RKIAAWSIAL VCIGVLGLAS
AASAERLPSF PRFTICGVSA VILLGSLITL GYIDEDEERH NFGHKGGFLC PFVPYLPVLC
ILINTYLIIN IGAGTWIRVL IWLLIGSMIY IFYGRSHSLL NNAVYVPTMT CTRKTTDHLA
