CAAT5_ARATH
ID CAAT5_ARATH Reviewed; 569 AA.
AC O64759; Q8RX58;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Cationic amino acid transporter 5;
GN Name=CAT5; OrderedLocusNames=At2g34960; ORFNames=F19I3.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, BIOPHYSICOCHEMICAL PROPERTIES, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, GENE FAMILY, AND NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15377779; DOI=10.1104/pp.104.045278;
RA Su Y.-H., Frommer W.B., Ludewig U.;
RT "Molecular and functional characterization of a family of amino acid
RT transporters from Arabidopsis.";
RL Plant Physiol. 136:3104-3113(2004).
CC -!- FUNCTION: High-affinity permease involved in the transport of the
CC cationic amino acids (e.g. arginine, and, to a lower extent, citrulline
CC and glutamate). Transport mostly basic amino acids, and, to a lower
CC extent neutral and acidic amino acids. {ECO:0000269|PubMed:15377779}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=12.0 uM for arginine {ECO:0000269|PubMed:15377779};
CC pH dependence:
CC Optimal transport of arginine at pH 5. {ECO:0000269|PubMed:15377779};
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15377779};
CC Multi-pass membrane protein {ECO:0000269|PubMed:15377779}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, flowers, seeds, and
CC leaves. Mostly present in leaf rims and cotyledons of developing
CC seedlings. {ECO:0000269|PubMed:15377779}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL91292.1; Type=Frameshift; Evidence={ECO:0000305};
CC Sequence=AAN18189.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AC004238; AAC12835.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC09043.1; -; Genomic_DNA.
DR EMBL; AY090448; AAL91292.1; ALT_FRAME; mRNA.
DR EMBL; BT000623; AAN18189.1; ALT_FRAME; mRNA.
DR PIR; T00477; T00477.
DR RefSeq; NP_181041.1; NM_129048.3.
DR AlphaFoldDB; O64759; -.
DR SMR; O64759; -.
DR BioGRID; 3406; 1.
DR IntAct; O64759; 1.
DR STRING; 3702.AT2G34960.1; -.
DR TCDB; 2.A.3.3.12; the amino acid-polyamine-organocation (apc) family.
DR PaxDb; O64759; -.
DR PRIDE; O64759; -.
DR ProteomicsDB; 240538; -.
DR EnsemblPlants; AT2G34960.1; AT2G34960.1; AT2G34960.
DR GeneID; 818060; -.
DR Gramene; AT2G34960.1; AT2G34960.1; AT2G34960.
DR KEGG; ath:AT2G34960; -.
DR Araport; AT2G34960; -.
DR TAIR; locus:2044682; AT2G34960.
DR eggNOG; KOG1286; Eukaryota.
DR HOGENOM; CLU_007946_15_9_1; -.
DR InParanoid; O64759; -.
DR OMA; TYVAGCF; -.
DR OrthoDB; 439017at2759; -.
DR PhylomeDB; O64759; -.
DR PRO; PR:O64759; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; O64759; baseline and differential.
DR Genevisible; O64759; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0005313; F:L-glutamate transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0015189; F:L-lysine transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR029485; CAT_C.
DR Pfam; PF13520; AA_permease_2; 1.
DR Pfam; PF13906; AA_permease_C; 1.
PE 1: Evidence at protein level;
KW Amino-acid transport; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..569
FT /note="Cationic amino acid transporter 5"
FT /id="PRO_0000415781"
FT TOPO_DOM 1..67
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 68..88
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 89..97
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 98..118
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 119..143
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 144..164
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 165..192
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 214..222
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 223..243
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 244..251
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 252..272
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 273..290
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 291..311
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 312..341
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 342..362
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 363..389
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 390..410
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 411
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 412..432
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 433..450
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 451..471
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 472..477
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 478..498
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 499..505
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 506..526
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 527..537
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 538..558
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 559..569
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
SQ SEQUENCE 569 AA; 62636 MW; 3234EB8CD900A378 CRC64;
MEGEERGYWR WSKRDFFPEE SFQSFGSYRA ALSQTCSRFK NRLVSRSDDE NERFELKKQS
EHEMKRCLTW WDLVWFGFGS VIGAGIFVLT GQEAHEQAGP AIVLSYVVSG LSAMLSVFCY
TEFAVEIPVA GGSFAYLRIE LGDFAAFITA GNILLESIVG TAAVARAWTS YFATLLNRSP
NALRIKTDLS SGFNLLDPIA VVVIAASATI ASISTRKTSL LNWIASAINT LVIFFVIIAG
FIHADTSNLT PFLPFGPEGV FRAAAVVYFA YGGFDSIATM AEETKNPSRD IPIGLLGSMS
IITVIYCLMA LSLSMMQKYT DIDPNAAYSV AFQSVGMKWG KYLVALGALK GMTTVLLVGA
LGQARYVTHI ARTHMIPPIF ALVHPKTGTP INANLLVAIP SALIAFFSGL DVLASLLSIS
TLFIFTMMPI ALLVRRYYVR QDTPRVHLIK LITCLLFVVV SSMGTSAYWG MQRKGSWIGY
TVTVPFWFLG TLGIVFFVPQ QRTPKVWGVP LVPWLPCLSI ATNIFLMGSL GAMAFVRFGV
CTLAMLLYYF LLGLHATFDM AHQQIVPRT
