CAAT8_ARATH
ID CAAT8_ARATH Reviewed; 590 AA.
AC Q9SHH0;
DT 22-FEB-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 140.
DE RecName: Full=Cationic amino acid transporter 8, vacuolar;
GN Name=CAT8; OrderedLocusNames=At1g17120; ORFNames=F20D23.19;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130712; DOI=10.1038/35048500;
RA Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL Nature 408:816-820(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP FUNCTION, TISSUE SPECIFICITY, SUBCELLULAR LOCATION, GENE FAMILY, AND
RP NOMENCLATURE.
RC STRAIN=cv. Columbia;
RX PubMed=15377779; DOI=10.1104/pp.104.045278;
RA Su Y.-H., Frommer W.B., Ludewig U.;
RT "Molecular and functional characterization of a family of amino acid
RT transporters from Arabidopsis.";
RL Plant Physiol. 136:3104-3113(2004).
CC -!- FUNCTION: Permease involved in the transport of the cationic neutral or
CC acidic amino acids. {ECO:0000269|PubMed:15377779}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:15377779};
CC Multi-pass membrane protein {ECO:0000269|PubMed:15377779}.
CC -!- TISSUE SPECIFICITY: Expressed in roots, stems, flowers and leaves.
CC Mostly present in young and rapidly dividing tissues such as the shoot
CC and root apical meristem, and in young leaves and petioles during
CC seedling development. {ECO:0000269|PubMed:15377779}.
CC -!- SIMILARITY: Belongs to the amino acid-polyamine-organocation (APC)
CC superfamily. Cationic amino acid transporter (CAT) (TC 2.A.3.3) family.
CC {ECO:0000305}.
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DR EMBL; AC007651; AAD50030.1; -; Genomic_DNA.
DR EMBL; CP002684; AEE29544.1; -; Genomic_DNA.
DR EMBL; AY072181; AAL60003.1; -; mRNA.
DR EMBL; AY117296; AAM51371.1; -; mRNA.
DR PIR; A86307; A86307.
DR RefSeq; NP_173155.1; NM_101572.4.
DR AlphaFoldDB; Q9SHH0; -.
DR SMR; Q9SHH0; -.
DR STRING; 3702.AT1G17120.1; -.
DR TCDB; 2.A.3.3.11; the amino acid-polyamine-organocation (apc) family.
DR iPTMnet; Q9SHH0; -.
DR PaxDb; Q9SHH0; -.
DR PRIDE; Q9SHH0; -.
DR ProteomicsDB; 240309; -.
DR EnsemblPlants; AT1G17120.1; AT1G17120.1; AT1G17120.
DR GeneID; 838282; -.
DR Gramene; AT1G17120.1; AT1G17120.1; AT1G17120.
DR KEGG; ath:AT1G17120; -.
DR Araport; AT1G17120; -.
DR TAIR; locus:2020387; AT1G17120.
DR eggNOG; KOG1286; Eukaryota.
DR HOGENOM; CLU_007946_15_9_1; -.
DR InParanoid; Q9SHH0; -.
DR OMA; FFICSAV; -.
DR OrthoDB; 439017at2759; -.
DR PhylomeDB; Q9SHH0; -.
DR PRO; PR:Q9SHH0; -.
DR Proteomes; UP000006548; Chromosome 1.
DR ExpressionAtlas; Q9SHH0; baseline and differential.
DR Genevisible; Q9SHH0; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005886; C:plasma membrane; IDA:TAIR.
DR GO; GO:0015171; F:amino acid transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0006865; P:amino acid transport; IBA:GO_Central.
DR InterPro; IPR002293; AA/rel_permease1.
DR InterPro; IPR029485; CAT_C.
DR Pfam; PF13520; AA_permease_2; 1.
DR Pfam; PF13906; AA_permease_C; 1.
PE 2: Evidence at transcript level;
KW Amino-acid transport; Cell membrane; Glycoprotein; Membrane;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..590
FT /note="Cationic amino acid transporter 8, vacuolar"
FT /id="PRO_0000415784"
FT TOPO_DOM 1..85
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..114
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..160
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 161..181
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 182..209
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 210..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 231..238
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 239..259
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 260..266
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 267..287
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 288..310
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 311..331
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 332..359
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 360..380
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 381..407
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 408..428
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 429
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 430..450
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 451..465
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 466..486
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 487..493
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 494..514
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 515..522
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 523..543
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 544..553
FT /note="Vacuolar"
FT /evidence="ECO:0000255"
FT TRANSMEM 554..574
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 575..590
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 195
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 590 AA; 64862 MW; 9205D33EEEC4F7F7 CRC64;
MIPASMEEAH QLESRSDDLS QRRSYWRWRK QDFFPEFSFQ SFSTYKSALS ATCPRLADRL
LSRSSDAYEL DAARRESENP MRRCLTWWDL LWLSFGSVVG SGVFVITGQE ARVGAGPAVV
LSYAISGVSA LLSVLCYAEF GVEIPVAGGS FSYLRVELGD FIAFIAAGNI LLEAMVGAAG
LGRSWSSYLA SLVKNDSDYF RIKVDSFAKG FDLLDPVAVA VLLVANGIAM TGTKRTSWLN
LITSMVTVCI IVFIVVVGFT HSKTSNLVPF FPYGAKGVVQ SAAVVYWSYT GFDMVANMAE
ETEKPSRDIP IGLVGSMSMI TVVYCLMALA LTMMVKYTEI DANAAYSVAF AQIGMKWAKY
LVGICALKGM TTSLLVGSLG QARYTTQIAR SHMIPPWFAL VHPKTGTPIY ATLLVTILSS
IISFFTSLEV LSSVFSFATL FIFMLVAVAL LVRRYYVKDV TPEAGLLKFL GFLFLIIASS
IGVSALWNSG VKGWIAYTVT GVIWFIGTLG LALLPKYRVP KVWGVPLVPW LPSFSIAMNL
FLIGSLGYVA FLRFIICTMV MLLYYLFVGL HATYDVAHQP LEEAKFEGER
