UPP_MOUSE
ID UPP_MOUSE Reviewed; 310 AA.
AC B1AVZ0;
DT 19-OCT-2011, integrated into UniProtKB/Swiss-Prot.
DT 08-APR-2008, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Uracil phosphoribosyltransferase homolog;
GN Name=Uprt;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-25, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Lung, Pancreas, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}. Nucleus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the UPRTase family. {ECO:0000305}.
CC -!- CAUTION: The uracil binding region known from UPRTases is missing.
CC {ECO:0000305}.
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DR EMBL; AL731717; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC147845; AAI47846.1; -; mRNA.
DR EMBL; BC147853; AAI47854.1; -; mRNA.
DR CCDS; CCDS41093.1; -.
DR RefSeq; NP_001074658.1; NM_001081189.1.
DR AlphaFoldDB; B1AVZ0; -.
DR SMR; B1AVZ0; -.
DR STRING; 10090.ENSMUSP00000085175; -.
DR iPTMnet; B1AVZ0; -.
DR PhosphoSitePlus; B1AVZ0; -.
DR EPD; B1AVZ0; -.
DR jPOST; B1AVZ0; -.
DR MaxQB; B1AVZ0; -.
DR PaxDb; B1AVZ0; -.
DR PeptideAtlas; B1AVZ0; -.
DR PRIDE; B1AVZ0; -.
DR ProteomicsDB; 298126; -.
DR Antibodypedia; 361; 285 antibodies from 19 providers.
DR Ensembl; ENSMUST00000087867; ENSMUSP00000085175; ENSMUSG00000073016.
DR GeneID; 331487; -.
DR KEGG; mmu:331487; -.
DR UCSC; uc009uai.1; mouse.
DR CTD; 139596; -.
DR MGI; MGI:2685620; Uprt.
DR VEuPathDB; HostDB:ENSMUSG00000073016; -.
DR eggNOG; KOG1017; Eukaryota.
DR GeneTree; ENSGT01020000230412; -.
DR HOGENOM; CLU_067096_1_0_1; -.
DR InParanoid; B1AVZ0; -.
DR OMA; NLFCTPM; -.
DR OrthoDB; 929897at2759; -.
DR PhylomeDB; B1AVZ0; -.
DR TreeFam; TF105900; -.
DR BioGRID-ORCS; 331487; 1 hit in 71 CRISPR screens.
DR PRO; PR:B1AVZ0; -.
DR Proteomes; UP000000589; Chromosome X.
DR RNAct; B1AVZ0; protein.
DR Bgee; ENSMUSG00000073016; Expressed in jejunum and 58 other tissues.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; ISO:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0006222; P:UMP biosynthetic process; ISO:MGI.
DR CDD; cd06223; PRTases_typeI; 1.
DR Gene3D; 3.40.50.2020; -; 1.
DR InterPro; IPR000836; PRibTrfase_dom.
DR InterPro; IPR029057; PRTase-like.
DR SUPFAM; SSF53271; SSF53271; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; GTP-binding; Nucleotide-binding; Nucleus; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..310
FT /note="Uracil phosphoribosyltransferase homolog"
FT /id="PRO_0000413215"
FT REGION 1..27
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 62..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 10..27
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 64..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 134
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q26998"
FT BINDING 143
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q26998"
FT BINDING 177..180
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q26998"
FT BINDING 187
FT /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
FT /ligand_id="ChEBI:CHEBI:58017"
FT /evidence="ECO:0000250|UniProtKB:Q26998"
FT BINDING 204
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q26998"
FT BINDING 233
FT /ligand="GTP"
FT /ligand_id="ChEBI:CHEBI:37565"
FT /evidence="ECO:0000250|UniProtKB:Q26998"
FT BINDING 239..247
FT /ligand="5-phospho-alpha-D-ribose 1-diphosphate"
FT /ligand_id="ChEBI:CHEBI:58017"
FT /evidence="ECO:0000250|UniProtKB:Q26998"
FT BINDING 300..302
FT /ligand="uracil"
FT /ligand_id="ChEBI:CHEBI:17568"
FT /evidence="ECO:0000250|UniProtKB:Q26998"
FT MOD_RES 25
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
SQ SEQUENCE 310 AA; 34278 MW; 5EB768287D80B879 CRC64;
MASELQRPDS MPCHNRQVNS TSSPSPEHLL AEDRVLDHAE ENNAAMAKLT LLPGHAHSSV
LSERDSPACC STNLHSENHS DSSDSGNYDA PVGGDSLLGD CELSRQIGAQ LKLLPMNDQI
RELQTIIRDK TASRGDFMFS ADRLIRLVVE EGLNQLPYKE CMVTTPTGHK YEGVKFEKGN
CGVSIMRSGE AMEQGLRDCC RSIRIGKILI QSDEETQRAK VYYAKFPPDI HRRKVLLMYP
ILSTGNTVIE AVKVLIEHGV QPSVIILLSL FSTPHGAKSI IQEFPEITIL TTEVHPVAPT
HFGQKYFGTD
