CABS1_MOUSE
ID CABS1_MOUSE Reviewed; 391 AA.
AC Q8C633; Q9D4L4;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Calcium-binding and spermatid-specific protein 1;
GN Name=Cabs1;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Testis;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP CALCIUM-BINDING, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, AND INDUCTION BY
RP BUSULFAN.
RX PubMed=19208547; DOI=10.1095/biolreprod.108.073866;
RA Kawashima A., Osman B.A., Takashima M., Kikuchi A., Kohchi S., Satoh E.,
RA Tamba M., Matsuda M., Okamura N.;
RT "CABS1 is a novel calcium-binding protein specifically expressed in
RT elongate spermatids of mice.";
RL Biol. Reprod. 80:1293-1304(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE, AND TISSUE
RP SPECIFICITY.
RX PubMed=33440775; DOI=10.3390/ijms22020652;
RA Zhang X., Zhou W., Zhang P., Gao F., Zhao X., Shum W.W., Zeng X.;
RT "Cabs1 Maintains Structural Integrity of Mouse Sperm Flagella during
RT Epididymal Transit of Sperm.";
RL Int. J. Mol. Sci. 22:0-0(2021).
CC -!- FUNCTION: Calcium-binding protein (PubMed:19208547). Essential for
CC maintaining the structural integrity of the sperm flagella
CC (PubMed:33440775). {ECO:0000269|PubMed:19208547,
CC ECO:0000269|PubMed:33440775}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q68FX6}.
CC Mitochondrion inner membrane {ECO:0000250|UniProtKB:Q68FX6}. Cell
CC projection, cilium, flagellum {ECO:0000269|PubMed:19208547,
CC ECO:0000269|PubMed:33440775}. Cytoplasmic vesicle, secretory vesicle,
CC acrosome {ECO:0000269|PubMed:33440775}. Note=Mostly cytoplasmic, but
CC associated with the mitochondrial inner membrane during the last steps
CC of spermatid differentiation (By similarity). Localizes to the
CC principal piece of the sperm flagellum (PubMed:19208547,
CC PubMed:33440775). {ECO:0000250|UniProtKB:Q68FX6,
CC ECO:0000269|PubMed:19208547, ECO:0000269|PubMed:33440775}.
CC -!- TISSUE SPECIFICITY: Detected only in testis. Expressed from stages X to
CC VIII of the seminiferous epithelial cycle. Expressed from step 13 to
CC step 16 of spermatid development (at protein level).
CC {ECO:0000269|PubMed:19208547, ECO:0000269|PubMed:33440775}.
CC -!- INDUCTION: Down-regulated by Busulfan. {ECO:0000269|PubMed:19208547}.
CC -!- DISRUPTION PHENOTYPE: Males exhibit significantly impaired sperm tail
CC structure and subfertility (PubMed:33440775). Defects in sperm
CC flagellar differentiation leads to an abnormal annulus and
CC disorganization of the midpiece-principal piece junction
CC (PubMed:33440775). {ECO:0000269|PubMed:33440775}.
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DR EMBL; AK016441; BAB30237.1; -; mRNA.
DR EMBL; AK076636; BAC36426.1; -; mRNA.
DR EMBL; BC049750; AAH49750.1; -; mRNA.
DR CCDS; CCDS39136.1; -.
DR RefSeq; NP_081907.1; NM_027631.2.
DR AlphaFoldDB; Q8C633; -.
DR STRING; 10090.ENSMUSP00000008051; -.
DR iPTMnet; Q8C633; -.
DR PhosphoSitePlus; Q8C633; -.
DR REPRODUCTION-2DPAGE; Q8C633; -.
DR PaxDb; Q8C633; -.
DR PeptideAtlas; Q8C633; -.
DR PRIDE; Q8C633; -.
DR ProteomicsDB; 281738; -.
DR Antibodypedia; 44320; 57 antibodies from 21 providers.
DR DNASU; 70977; -.
DR Ensembl; ENSMUST00000008051; ENSMUSP00000008051; ENSMUSG00000007907.
DR GeneID; 70977; -.
DR KEGG; mmu:70977; -.
DR UCSC; uc008xzj.1; mouse.
DR CTD; 85438; -.
DR MGI; MGI:1918227; Cabs1.
DR VEuPathDB; HostDB:ENSMUSG00000007907; -.
DR eggNOG; ENOG502RWWC; Eukaryota.
DR GeneTree; ENSGT00390000015647; -.
DR HOGENOM; CLU_719537_0_0_1; -.
DR InParanoid; Q8C633; -.
DR OMA; KITEPFS; -.
DR OrthoDB; 1176309at2759; -.
DR PhylomeDB; Q8C633; -.
DR TreeFam; TF338174; -.
DR BioGRID-ORCS; 70977; 2 hits in 72 CRISPR screens.
DR PRO; PR:Q8C633; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q8C633; protein.
DR Bgee; ENSMUSG00000007907; Expressed in seminiferous tubule of testis and 9 other tissues.
DR Genevisible; Q8C633; MM.
DR GO; GO:0001669; C:acrosomal vesicle; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005743; C:mitochondrial inner membrane; ISO:MGI.
DR GO; GO:0031514; C:motile cilium; IDA:UniProtKB.
DR GO; GO:0097228; C:sperm principal piece; IDA:UniProtKB.
DR GO; GO:0005509; F:calcium ion binding; IDA:UniProtKB.
DR GO; GO:0030317; P:flagellated sperm motility; IMP:UniProtKB.
DR GO; GO:0007283; P:spermatogenesis; IBA:GO_Central.
DR InterPro; IPR026118; Ca-bd_spermatid.
DR PANTHER; PTHR22810; PTHR22810; 2.
DR Pfam; PF15367; CABS1; 1.
PE 1: Evidence at protein level;
KW Calcium; Cell projection; Cilium; Cytoplasm; Cytoplasmic vesicle;
KW Flagellum; Membrane; Mitochondrion; Mitochondrion inner membrane;
KW Phosphoprotein; Reference proteome.
FT CHAIN 1..391
FT /note="Calcium-binding and spermatid-specific protein 1"
FT /id="PRO_0000339180"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 90..110
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 152..221
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 271..299
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 330..352
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 91..110
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 181..198
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 253
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q68FX6"
FT MOD_RES 269
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q68FX6"
FT MOD_RES 314
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q68FX6"
FT MOD_RES 347
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q68FX6"
FT MOD_RES 357
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q68FX6"
FT MOD_RES 372
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q68FX6"
FT MOD_RES 376
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:Q68FX6"
FT CONFLICT 8
FT /note="K -> Q (in Ref. 1; BAB30237)"
FT /evidence="ECO:0000305"
FT CONFLICT 108
FT /note="S -> V (in Ref. 1; BAB30237)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 391 AA; 42302 MW; F4E8FFFC0DFF51A8 CRC64;
MAEDGSPKIY SRPPRDNSKT PTEADIFFGA DNTIPKSETT ITSEGDHVTS VNDCTPDGDF
STTVNKLTPT KEKLKLEDDI EGCLKLTTLP EKEITTPTET PNSKPKGSIT ENFIPVKIGN
TSSPVGTVSL IDFSSNTAKE DIFLTTIDTG EKEVVPTTEF SGTLEDSAAD VEDASGFPDE
STETDVPSSA TSDAPDDGAV QVTDSFSPEA GVPPSTEKEV TTIPDITNIA EENVTEIKLI
VSEDRPKTVT KLSDSEEEKF ITVFELTNSA EKAKDNVEDP LNDEESTDGA NDWMEKETAS
EAESHAVLLT AVESRYDFIV TASETDNVME ESHVNTTDLP ENETTESVTN VTEELPSVTS
IVDTLKDKED LSTTNSGLFK LLKEEPDDLM M
