UPS4_ARATH
ID UPS4_ARATH Reviewed; 401 AA.
AC Q9ZQ88; Q1PFA1; Q6E273; Q6E274; Q6E275;
DT 05-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2005, sequence version 2.
DT 03-AUG-2022, entry version 112.
DE RecName: Full=Ureide permease 4 {ECO:0000303|PubMed:11971139};
DE Short=AtUPS4 {ECO:0000303|PubMed:11971139};
GN Name=UPS4 {ECO:0000303|PubMed:11971139};
GN OrderedLocusNames=At2g03520 {ECO:0000312|Araport:AT2G03520};
GN ORFNames=T4M8.4 {ECO:0000312|EMBL:AAD17425.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 2; 3 AND 4).
RC STRAIN=cv. Columbia;
RA Xiao Y.-L., Underwood B.A., Moskal W.A. Jr., Torian U., Redman J.C.,
RA Wu H.C., Utterback T., Town C.D.;
RT "Reconstruction of cDNA sequences for hypothetical genes in Arabidopsis
RT thaliana from 5' and 3' RACE products.";
RL Submitted (APR-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [5]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=11971139; DOI=10.1105/tpc.010458;
RA Desimone M., Catoni E., Ludewig U., Hilpert M., Schneider A., Kunze R.,
RA Tegeder M., Frommer W.B., Schumacher K.;
RT "A novel superfamily of transporters for allantoin and other oxo
RT derivatives of nitrogen heterocyclic compounds in Arabidopsis.";
RL Plant Cell 14:847-856(2002).
RN [6]
RP TISSUE SPECIFICITY.
RX PubMed=16738859; DOI=10.1007/s00425-006-0315-z;
RA Schmidt A., Baumann N., Schwarzkopf A., Frommer W.B., Desimone M.;
RT "Comparative studies on Ureide Permeases in Arabidopsis thaliana and
RT analysis of two alternative splice variants of AtUPS5.";
RL Planta 224:1329-1340(2006).
CC -!- FUNCTION: Proton-coupled transporter that transports a wide spectrum of
CC oxo derivatives of heterocyclic nitrogen compounds.
CC {ECO:0000250|UniProtKB:Q9ZPR7}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Multi-pass membrane
CC protein {ECO:0000255}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=4;
CC Name=1;
CC IsoId=Q9ZQ88-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9ZQ88-2; Sequence=VSP_014325, VSP_014326;
CC Name=3;
CC IsoId=Q9ZQ88-3; Sequence=VSP_014323, VSP_014324;
CC Name=4;
CC IsoId=Q9ZQ88-4; Sequence=VSP_014322, VSP_014323, VSP_014324;
CC -!- TISSUE SPECIFICITY: Expressed in developing seedlings, flower filaments
CC and stigma, and the top and bottom parts of carpels in siliques.
CC {ECO:0000269|PubMed:16738859}.
CC -!- SIMILARITY: Belongs to the plant ureide permease (TC 2.A.7.19) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAD17425.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAT68347.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AC006284; AAD17425.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP002685; AEC05710.1; -; Genomic_DNA.
DR EMBL; AY600550; AAT68349.1; -; mRNA.
DR EMBL; AY600549; AAT68348.1; -; mRNA.
DR EMBL; AY600548; AAT68347.1; ALT_INIT; mRNA.
DR EMBL; DQ446462; ABE65798.1; -; mRNA.
DR PIR; D84449; D84449.
DR RefSeq; NP_178450.2; NM_126402.3. [Q9ZQ88-1]
DR AlphaFoldDB; Q9ZQ88; -.
DR STRING; 3702.AT2G03520.1; -.
DR PaxDb; Q9ZQ88; -.
DR PRIDE; Q9ZQ88; -.
DR ProteomicsDB; 228564; -. [Q9ZQ88-1]
DR EnsemblPlants; AT2G03520.1; AT2G03520.1; AT2G03520. [Q9ZQ88-1]
DR GeneID; 814881; -.
DR Gramene; AT2G03520.1; AT2G03520.1; AT2G03520. [Q9ZQ88-1]
DR KEGG; ath:AT2G03520; -.
DR Araport; AT2G03520; -.
DR TAIR; locus:2063851; AT2G03520.
DR eggNOG; ENOG502QUAA; Eukaryota.
DR HOGENOM; CLU_051261_0_0_1; -.
DR InParanoid; Q9ZQ88; -.
DR OMA; GRGWAVF; -.
DR PhylomeDB; Q9ZQ88; -.
DR PRO; PR:Q9ZQ88; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q9ZQ88; baseline and differential.
DR Genevisible; Q9ZQ88; AT.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0005274; F:allantoin:proton symporter activity; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0015505; F:uracil:cation symporter activity; IBA:GO_Central.
DR InterPro; IPR030189; UPS_plant.
DR InterPro; IPR009834; Ureide_permease.
DR PANTHER; PTHR31081; PTHR31081; 1.
DR Pfam; PF07168; Ureide_permease; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; ATP-binding; Membrane; Nucleotide-binding;
KW Reference proteome; Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..401
FT /note="Ureide permease 4"
FT /id="PRO_0000221648"
FT TOPO_DOM 1..10
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 11..31
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 32..40
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 41..61
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 62..81
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..104
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..141
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 142..162
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 163..231
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 232..252
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 253..275
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 276..296
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 297..318
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 319..339
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 340..344
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 345..365
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 366..374
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 375..395
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 396..401
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT BINDING 224..231
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VAR_SEQ 126
FT /note="G -> GILPLVCDESKTYVSMVSCVSRLNFIG (in isoform 4)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_014322"
FT VAR_SEQ 179..184
FT /note="DLYSSI -> YKAETN (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:17147637, ECO:0000303|Ref.3"
FT /id="VSP_014323"
FT VAR_SEQ 185..401
FT /note="Missing (in isoform 3 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:17147637, ECO:0000303|Ref.3"
FT /id="VSP_014324"
FT VAR_SEQ 228..230
FT /note="FGK -> QLQ (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_014325"
FT VAR_SEQ 231..401
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_014326"
SQ SEQUENCE 401 AA; 43203 MW; FBEC7511B34A0154 CRC64;
MYVVESKAGA IGCMILSLCC LGSWPAILTL LERRGRLPQH TFLDFATANL LAAIVIAFSL
GEIGKSTFLK PDFTTQLPQD NWPSVLLAVA GGVLLSIGNL ATQYAFAFVG LSVTEVITAS
ITVVIGTTLN YFLDNKINKA EILFPGVGCF LIAVFLGAAV HASNAADVKE KLKSLPSEDL
YSSIENGEDK PEIEKTDVES QEKLAEKAKA GTAGFYVELE NKRAIKVFGK SIMIGLFITL
FAGISLSLFS PAFNLATNDQ WSTLPKGVPK LVVYTAFFYF SIAGFLISLI LNLIFLYRPM
VGLARSSLKK YIYDSKGRGW AVFAGFLCGF GNGLQFMGGQ AAGYAAADSV QALPLVSTFW
GIVLFGEYRK SSKRTYALLV SMLAMFVAAV AILMASSGHR K
