URAA_HAEIN
ID URAA_HAEIN Reviewed; 414 AA.
AC P45117;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 123.
DE RecName: Full=Probable uracil permease {ECO:0000250|UniProtKB:P0AGM7};
DE AltName: Full=Uracil transporter {ECO:0000250|UniProtKB:P0AGM7};
DE AltName: Full=Uracil/H(+) symporter UraA {ECO:0000250|UniProtKB:P0AGM7};
GN Name=uraA; OrderedLocusNames=HI_1227;
OS Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales;
OC Pasteurellaceae; Haemophilus.
OX NCBI_TaxID=71421;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX PubMed=7542800; DOI=10.1126/science.7542800;
RA Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT Rd.";
RL Science 269:496-512(1995).
CC -!- FUNCTION: Transport of uracil in the cell.
CC {ECO:0000250|UniProtKB:P0AGM7}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H(+)(in) + uracil(in) = H(+)(out) + uracil(out);
CC Xref=Rhea:RHEA:29239, ChEBI:CHEBI:15378, ChEBI:CHEBI:17568;
CC Evidence={ECO:0000250|UniProtKB:P0AGM7};
CC PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:29241;
CC Evidence={ECO:0000250|UniProtKB:P0AGM7};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane
CC {ECO:0000250|UniProtKB:P0AGM7}; Multi-pass membrane protein
CC {ECO:0000250|UniProtKB:P0AGM7}.
CC -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC 2.A.40) family. {ECO:0000305}.
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DR EMBL; L42023; AAC22880.1; -; Genomic_DNA.
DR PIR; D64111; D64111.
DR RefSeq; NP_439383.1; NC_000907.1.
DR RefSeq; WP_005694289.1; NC_000907.1.
DR AlphaFoldDB; P45117; -.
DR SMR; P45117; -.
DR STRING; 71421.HI_1227; -.
DR DNASU; 950172; -.
DR EnsemblBacteria; AAC22880; AAC22880; HI_1227.
DR KEGG; hin:HI_1227; -.
DR PATRIC; fig|71421.8.peg.1279; -.
DR eggNOG; COG2233; Bacteria.
DR HOGENOM; CLU_017959_1_2_6; -.
DR OMA; INHGIGM; -.
DR PhylomeDB; P45117; -.
DR BioCyc; HINF71421:G1GJ1-1258-MON; -.
DR Proteomes; UP000000579; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015205; F:nucleobase transmembrane transporter activity; IEA:UniProt.
DR GO; GO:0015293; F:symporter activity; IEA:UniProtKB-KW.
DR InterPro; IPR006043; NCS2.
DR InterPro; IPR006042; Xan_ur_permease.
DR Pfam; PF00860; Xan_ur_permease; 1.
DR TIGRFAMs; TIGR00801; ncs2; 1.
DR PROSITE; PS01116; XANTH_URACIL_PERMASE; 1.
PE 3: Inferred from homology;
KW Cell inner membrane; Cell membrane; Membrane; Reference proteome; Symport;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..414
FT /note="Probable uracil permease"
FT /id="PRO_0000165960"
FT TOPO_DOM 1..14
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 15..38
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 39..42
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 43..62
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 63..65
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 66..82
FT /note="Discontinuously helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 83..91
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 113..124
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 125..146
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 147..155
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 156..171
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 172..178
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 179..199
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 200..224
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 225..248
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 249..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 262..281
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 282..298
FT /note="Discontinuously helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 299..301
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 302..319
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 320..332
FT /note="Periplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TRANSMEM 333..354
FT /note="Helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 355..365
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT INTRAMEM 366..401
FT /note="Discontinuously helical"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT TOPO_DOM 402..414
FT /note="Cytoplasmic"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT BINDING 74
FT /ligand="uracil"
FT /ligand_id="ChEBI:CHEBI:17568"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT BINDING 241
FT /ligand="uracil"
FT /ligand_id="ChEBI:CHEBI:17568"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
FT BINDING 290
FT /ligand="uracil"
FT /ligand_id="ChEBI:CHEBI:17568"
FT /evidence="ECO:0000250|UniProtKB:P0AGM7"
SQ SEQUENCE 414 AA; 43572 MW; 25361ABC22FE08B7 CRC64;
MTNQIPPSLA ENQSKLKQSF VGLQMLFVAF GALVLVPLIT GLDSNTALLT AGVGTLLFQF
CTGKQVPIFL ASSFAFIAPI QYGVQTWGIA TTMGGLAFTG LVYFALSTLV KLRGAEALQR
FFPPVVVGPV IIIIGMGLAP IAVDMSLGKN SAYAYNDAVL VSMVTLLTTL SVAVFAKGLM
KLIPIMFGIT AGYILCLFLG LINFQPVIDA PWFSLPKLTT PEFNLEAILY MLPIAIAPAV
EHVGGIMAIS SVTGKDFLKK PGLHRTLLGD GIATAAASLV GGPPNTTYAE VTGAVMLTRN
FNPNIMTWAA VWAIAISFCG KVGAFLSTIP TIVMGGIMML VFGSIAVVGM STLIRGKVDV
TEARNLCIIS VVMTFGIGNM FVDVGNVSLK GISLCAIVAI ILNLVLPKAK NEVE
