ID   URAD_AMICA              Reviewed;         175 AA.
AC   Q0ZDF7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   22-AUG-2006, sequence version 1.
DT   03-AUG-2022, entry version 48.
DE   RecName: Full=2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase;
DE            Short=OHCU decarboxylase;
DE            EC=4.1.1.97;
DE   AltName: Full=Parahox neighbor;
DE   AltName: Full=Ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase;
GN   Name=urad; Synonyms=prhoxnb;
OS   Amia calva (Bowfin).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Holostei; Amiiformes; Amiidae; Amia.
OX   NCBI_TaxID=7924;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=16801555; DOI=10.1073/pnas.0600341103;
RA   Mulley J.F., Chiu C.-H., Holland P.W.H.;
RT   "Breakup of a homeobox cluster after genome duplication in teleosts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 103:10369-10372(2006).
CC   -!- FUNCTION: Catalyzes the stereoselective decarboxylation of 2-oxo-4-
CC       hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-
CC         carboxylate + H(+) = (S)-allantoin + CO2; Xref=Rhea:RHEA:26301,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15678, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:58639; EC=4.1.1.97;
CC   -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC       urate: step 3/3.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the OHCU decarboxylase family. {ECO:0000305}.
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DR   EMBL; DQ504303; ABF51667.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q0ZDF7; -.
DR   SMR; Q0ZDF7; -.
DR   UniPathway; UPA00394; UER00652.
DR   GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR   GO; GO:0051997; F:2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity; IEA:RHEA.
DR   GO; GO:0000255; P:allantoin metabolic process; IEA:InterPro.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019628; P:urate catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 1.10.3330.10; -; 1.
DR   InterPro; IPR018020; OHCU_decarboxylase.
DR   InterPro; IPR017580; OHCU_decarboxylase-1.
DR   InterPro; IPR036778; OHCU_decarboxylase_sf.
DR   Pfam; PF09349; OHCU_decarbox; 1.
DR   SUPFAM; SSF158694; SSF158694; 1.
DR   TIGRFAMs; TIGR03164; UHCUDC; 1.
PE   3: Inferred from homology;
KW   Decarboxylase; Lyase; Peroxisome; Purine metabolism.
FT   CHAIN           1..175
FT                   /note="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
FT                   decarboxylase"
FT                   /id="PRO_0000315242"
FT   MOTIF           173..175
FT                   /note="Microbody targeting signal"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        67
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         68
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         84..88
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         119..123
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   175 AA;  19501 MW;  B300C0DD3E8EB8B0 CRC64;
     MDMRQVNSLS YEEFVETFGN VVERCPVVAA AVWSGRPFAN SHDLEASIGE FIDSLPVAGQ
     EGIVRCHPDL AGRDLKRGAL TAESRGEQTQ AGLTLLDAGD AARMNRLNAQ YKERFGFPFV
     ICARMNNKEE ILLRLAERLG NEPAQERQCA IEEVKKICQL RLQDIIQCPA THTKL