ID   URAD_BOVIN              Reviewed;         170 AA.
AC   A5PJD0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   10-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 75.
DE   RecName: Full=2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase;
DE            Short=OHCU decarboxylase;
DE            EC=4.1.1.97;
DE   AltName: Full=Parahox neighbor;
DE   AltName: Full=Ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase;
GN   Name=URAD; Synonyms=PRHOXNB;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Testis;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the stereoselective decarboxylation of 2-oxo-4-
CC       hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.
CC       {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-
CC         carboxylate + H(+) = (S)-allantoin + CO2; Xref=Rhea:RHEA:26301,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15678, ChEBI:CHEBI:16526,
CC         ChEBI:CHEBI:58639; EC=4.1.1.97;
CC   -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC       urate: step 3/3.
CC   -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the OHCU decarboxylase family. {ECO:0000305}.
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DR   EMBL; BC142055; AAI42056.1; -; mRNA.
DR   RefSeq; NP_001093173.1; NM_001099703.2.
DR   AlphaFoldDB; A5PJD0; -.
DR   SMR; A5PJD0; -.
DR   STRING; 9913.ENSBTAP00000018362; -.
DR   PaxDb; A5PJD0; -.
DR   PRIDE; A5PJD0; -.
DR   GeneID; 518364; -.
DR   KEGG; bta:518364; -.
DR   CTD; 646625; -.
DR   eggNOG; KOG0848; Eukaryota.
DR   InParanoid; A5PJD0; -.
DR   OrthoDB; 1547390at2759; -.
DR   UniPathway; UPA00394; UER00652.
DR   Proteomes; UP000009136; Unplaced.
DR   GO; GO:0005777; C:peroxisome; IBA:GO_Central.
DR   GO; GO:0051997; F:2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity; IBA:GO_Central.
DR   GO; GO:0000255; P:allantoin metabolic process; IEA:InterPro.
DR   GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0019628; P:urate catabolic process; IBA:GO_Central.
DR   Gene3D; 1.10.3330.10; -; 1.
DR   InterPro; IPR018020; OHCU_decarboxylase.
DR   InterPro; IPR017580; OHCU_decarboxylase-1.
DR   InterPro; IPR036778; OHCU_decarboxylase_sf.
DR   Pfam; PF09349; OHCU_decarbox; 1.
DR   SUPFAM; SSF158694; SSF158694; 1.
DR   TIGRFAMs; TIGR03164; UHCUDC; 1.
PE   2: Evidence at transcript level;
KW   Decarboxylase; Lyase; Peroxisome; Purine metabolism; Reference proteome.
FT   CHAIN           1..170
FT                   /note="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
FT                   decarboxylase"
FT                   /id="PRO_0000315239"
FT   ACT_SITE        67
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   BINDING         68
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         84..88
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
FT   BINDING         119..123
FT                   /ligand="substrate"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   170 AA;  18942 MW;  07D2B9739582B42E CRC64;
     MDIEKVNSMD FGEFVDVFGN VIERCPLIAA AVWSKRPFSG LGDLEKHFFA FIDGLPLSGQ
     EGVLRCHPEL AGRQLPWGRL TAESQREQSA AGLQNLGAAE RLRFTELTAQ YRTRFGFPFV
     LALRLSDPAA APRELARRLR CPPAQELRTA LGEVKKICHL RLANLLGEQP