URAD_MOUSE
ID URAD_MOUSE Reviewed; 178 AA.
AC Q283N4;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 04-APR-2006, sequence version 1.
DT 03-AUG-2022, entry version 103.
DE RecName: Full=2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase;
DE Short=OHCU decarboxylase;
DE EC=4.1.1.97 {ECO:0000269|PubMed:16462750};
DE AltName: Full=Parahox neighbor;
DE AltName: Full=Ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase;
GN Name=Urad; Synonyms=Prhoxnb;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, AND CATALYTIC ACTIVITY.
RX PubMed=16462750; DOI=10.1038/nchembio768;
RA Ramazzina I., Folli C., Secchi A., Berni R., Percudani R.;
RT "Completing the uric acid degradation pathway through phylogenetic
RT comparison of whole genomes.";
RL Nat. Chem. Biol. 2:144-148(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Catalyzes the stereoselective decarboxylation of 2-oxo-4-
CC hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.
CC {ECO:0000269|PubMed:16462750}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-
CC carboxylate + H(+) = (S)-allantoin + CO2; Xref=Rhea:RHEA:26301,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15678, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:58639; EC=4.1.1.97;
CC Evidence={ECO:0000269|PubMed:16462750};
CC -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC urate: step 3/3.
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the OHCU decarboxylase family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; DQ225767; ABB46374.1; -; mRNA.
DR EMBL; BC152959; AAI52960.1; -; mRNA.
DR CCDS; CCDS39399.1; -.
DR RefSeq; NP_001034767.1; NM_001039678.2.
DR AlphaFoldDB; Q283N4; -.
DR SMR; Q283N4; -.
DR STRING; 10090.ENSMUSP00000098000; -.
DR iPTMnet; Q283N4; -.
DR PhosphoSitePlus; Q283N4; -.
DR SwissPalm; Q283N4; -.
DR jPOST; Q283N4; -.
DR MaxQB; Q283N4; -.
DR PaxDb; Q283N4; -.
DR PRIDE; Q283N4; -.
DR ProteomicsDB; 298255; -.
DR DNASU; 231903; -.
DR Ensembl; ENSMUST00000100433; ENSMUSP00000098000; ENSMUSG00000075543.
DR GeneID; 231903; -.
DR KEGG; mmu:231903; -.
DR UCSC; uc009aoa.2; mouse.
DR CTD; 646625; -.
DR MGI; MGI:3647519; Urad.
DR VEuPathDB; HostDB:ENSMUSG00000075543; -.
DR eggNOG; KOG0848; Eukaryota.
DR GeneTree; ENSGT00390000002395; -.
DR HOGENOM; CLU_092522_1_0_1; -.
DR InParanoid; Q283N4; -.
DR OMA; KICHLRL; -.
DR OrthoDB; 1547390at2759; -.
DR PhylomeDB; Q283N4; -.
DR TreeFam; TF323276; -.
DR UniPathway; UPA00394; UER00652.
DR BioGRID-ORCS; 231903; 3 hits in 71 CRISPR screens.
DR PRO; PR:Q283N4; -.
DR Proteomes; UP000000589; Chromosome 5.
DR RNAct; Q283N4; protein.
DR Bgee; ENSMUSG00000075543; Expressed in hepatobiliary system and 13 other tissues.
DR ExpressionAtlas; Q283N4; baseline and differential.
DR GO; GO:0005777; C:peroxisome; IDA:MGI.
DR GO; GO:0051997; F:2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity; IDA:MGI.
DR GO; GO:0016831; F:carboxy-lyase activity; IDA:MGI.
DR GO; GO:0006154; P:adenosine catabolic process; IDA:MGI.
DR GO; GO:0000255; P:allantoin metabolic process; IDA:MGI.
DR GO; GO:0006196; P:AMP catabolic process; IDA:MGI.
DR GO; GO:0046059; P:dAMP catabolic process; IDA:MGI.
DR GO; GO:0006157; P:deoxyadenosine catabolic process; IDA:MGI.
DR GO; GO:0006161; P:deoxyguanosine catabolic process; IDA:MGI.
DR GO; GO:0006149; P:deoxyinosine catabolic process; IDA:MGI.
DR GO; GO:0046055; P:dGMP catabolic process; IDA:MGI.
DR GO; GO:0046038; P:GMP catabolic process; IDA:MGI.
DR GO; GO:0006147; P:guanine catabolic process; IDA:MGI.
DR GO; GO:0009114; P:hypoxanthine catabolic process; IDA:MGI.
DR GO; GO:0006204; P:IMP catabolic process; IDA:MGI.
DR GO; GO:0006148; P:inosine catabolic process; IDA:MGI.
DR GO; GO:0019628; P:urate catabolic process; IDA:MGI.
DR GO; GO:0009115; P:xanthine catabolic process; IDA:MGI.
DR Gene3D; 1.10.3330.10; -; 1.
DR InterPro; IPR018020; OHCU_decarboxylase.
DR InterPro; IPR017580; OHCU_decarboxylase-1.
DR InterPro; IPR036778; OHCU_decarboxylase_sf.
DR Pfam; PF09349; OHCU_decarbox; 1.
DR SUPFAM; SSF158694; SSF158694; 1.
DR TIGRFAMs; TIGR03164; UHCUDC; 1.
PE 1: Evidence at protein level;
KW Decarboxylase; Lyase; Peroxisome; Purine metabolism; Reference proteome.
FT CHAIN 1..178
FT /note="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
FT decarboxylase"
FT /id="PRO_0000315241"
FT ACT_SITE 67
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 68
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 84..88
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 119..123
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 178 AA; 20017 MW; FDA1941E8BA89293 CRC64;
MDMVKVNSMD FGEFVDVFGN IVEKCPLIAA AVWSQRPFSG LEDLENHFFA FIDALPRSGQ
EGILRCHPDL AGRDLQQGTL TAESQREQSQ AGLTSLDTDD RLRLQQLNAQ YRERFGFPFV
LAARLSDRAT VPRELARRLQ CQPESELRTA LGEVKKISHL RLTDLLGAHS HSARVELP
