URAD_XENLA
ID URAD_XENLA Reviewed; 175 AA.
AC A1L1C5;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 60.
DE RecName: Full=2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase;
DE Short=OHCU decarboxylase;
DE EC=4.1.1.97;
DE AltName: Full=Parahox neighbor;
DE AltName: Full=Ureidoimidazoline (2-oxo-4-hydroxy-4-carboxy-5-) decarboxylase;
GN Name=urad; Synonyms=prhoxnb;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Kidney;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Catalyzes the stereoselective decarboxylation of 2-oxo-4-
CC hydroxy-4-carboxy-5-ureidoimidazoline (OHCU) to (S)-allantoin.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-
CC carboxylate + H(+) = (S)-allantoin + CO2; Xref=Rhea:RHEA:26301,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15678, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:58639; EC=4.1.1.97;
CC -!- PATHWAY: Purine metabolism; urate degradation; (S)-allantoin from
CC urate: step 3/3.
CC -!- SUBCELLULAR LOCATION: Peroxisome {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the OHCU decarboxylase family. {ECO:0000305}.
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DR EMBL; BC128985; AAI28986.1; -; mRNA.
DR RefSeq; NP_001091261.1; NM_001097792.1.
DR AlphaFoldDB; A1L1C5; -.
DR SMR; A1L1C5; -.
DR DNASU; 100037066; -.
DR GeneID; 100037066; -.
DR KEGG; xla:100037066; -.
DR CTD; 100037066; -.
DR Xenbase; XB-GENE-6255526; urad.L.
DR OMA; KICHLRL; -.
DR OrthoDB; 1547390at2759; -.
DR UniPathway; UPA00394; UER00652.
DR Proteomes; UP000186698; Chromosome 2L.
DR Bgee; 100037066; Expressed in kidney and 3 other tissues.
DR GO; GO:0005777; C:peroxisome; IEA:UniProtKB-SubCell.
DR GO; GO:0051997; F:2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase activity; IEA:RHEA.
DR GO; GO:0000255; P:allantoin metabolic process; IEA:InterPro.
DR GO; GO:0006144; P:purine nucleobase metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019628; P:urate catabolic process; IEA:UniProtKB-UniPathway.
DR Gene3D; 1.10.3330.10; -; 1.
DR InterPro; IPR018020; OHCU_decarboxylase.
DR InterPro; IPR017580; OHCU_decarboxylase-1.
DR InterPro; IPR036778; OHCU_decarboxylase_sf.
DR Pfam; PF09349; OHCU_decarbox; 1.
DR SUPFAM; SSF158694; SSF158694; 1.
DR TIGRFAMs; TIGR03164; UHCUDC; 1.
PE 2: Evidence at transcript level;
KW Decarboxylase; Lyase; Peroxisome; Purine metabolism; Reference proteome.
FT CHAIN 1..175
FT /note="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
FT decarboxylase"
FT /id="PRO_0000315244"
FT MOTIF 173..175
FT /note="Microbody targeting signal"
FT /evidence="ECO:0000255"
FT ACT_SITE 67
FT /note="Proton donor"
FT /evidence="ECO:0000250"
FT BINDING 68
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 84..88
FT /ligand="substrate"
FT /evidence="ECO:0000250"
FT BINDING 119..123
FT /ligand="substrate"
FT /evidence="ECO:0000250"
SQ SEQUENCE 175 AA; 19803 MW; 290EF6284C163CD0 CRC64;
MDLNTINSMS YEQFLDTFGN IIERCPLVTA AIWSQQPFAS VTELENRVFD FIESLPLAGK
EGILRCHPDL AGRDLMRGTL TDESQNEQAQ AGLTLLSAKE KETLGFLNLQ YKAKFGFPFV
ICAKMSDKNK IMQELGSRLQ NEQSEELQKG IAEVKKICHL RICDLFLNEK LPTKL
