ID   URAP_PAELB              Reviewed;         421 AA.
AC   A0A2A5K1W4;
DT   13-FEB-2019, integrated into UniProtKB/Swiss-Prot.
DT   20-DEC-2017, sequence version 1.
DT   03-AUG-2022, entry version 14.
DE   RecName: Full=Uracil permease {ECO:0000305};
DE   AltName: Full=Paenibacillus larvae uracil permease {ECO:0000303|PubMed:30157454};
DE            Short=PlUP {ECO:0000303|PubMed:30157454};
DE   AltName: Full=Uracil transporter {ECO:0000303|PubMed:30157454};
GN   ORFNames=PL1_0655 {ECO:0000312|EMBL:PCK71003.1};
OS   Paenibacillus larvae subsp. larvae (strain NRRL B-3650 / LMG 16245).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX   NCBI_TaxID=741161;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NRRL B-3650 / LMG 16245;
RX   PubMed=21923906; DOI=10.1186/1471-2164-12-450;
RA   Chan Q.W., Cornman R.S., Birol I., Liao N.Y., Chan S.K., Docking T.R.,
RA   Jackman S.D., Taylor G.A., Jones S.J., de Graaf D.C., Evans J.D.,
RA   Foster L.J.;
RT   "Updated genome assembly and annotation of Paenibacillus larvae, the agent
RT   of American foulbrood disease of honey bees.";
RL   BMC Genomics 12:450-450(2011).
RN   [2]
RP   FUNCTION, ACTIVITY REGULATION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC   STRAIN=NRRL B-3650 / LMG 16245;
RX   PubMed=30157454; DOI=10.1016/j.micpath.2018.08.059;
RA   Stoffer-Bittner A.J., Alexander C.R., Dingman D.W., Mourad G.S.,
RA   Schultes N.P.;
RT   "Functional characterization of the uracil transporter from honeybee
RT   pathogen Paenibacillus larvae.";
RL   Microb. Pathog. 124:305-310(2018).
CC   -!- FUNCTION: Specific for the uptake of uracil. Transport is probably
CC       proton-dependent. {ECO:0000269|PubMed:30157454}.
CC   -!- ACTIVITY REGULATION: Inhibited by the proton gradient disruptor
CC       carbonyl cyanide m-chlorophenylhydrazone (CCCP), but not by the sodium
CC       gradient disruptor ouabain. Both xanthine and uric acid act as
CC       competitive inhibitors of uracil transport.
CC       {ECO:0000269|PubMed:30157454}.
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=19.5 uM for uracil {ECO:0000269|PubMed:30157454};
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the nucleobase:cation symporter-2 (NCS2) (TC
CC       2.A.40) family. {ECO:0000305}.
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DR   EMBL; ADZY03000173; PCK71003.1; -; Genomic_DNA.
DR   RefSeq; WP_023482778.1; NZ_ADZY03000173.1.
DR   AlphaFoldDB; A0A2A5K1W4; -.
DR   SMR; A0A2A5K1W4; -.
DR   EnsemblBacteria; PCK71003; PCK71003; PL1_0655.
DR   Proteomes; UP000217691; Unassembled WGS sequence.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015205; F:nucleobase transmembrane transporter activity; IEA:UniProt.
DR   InterPro; IPR006043; NCS2.
DR   InterPro; IPR006042; Xan_ur_permease.
DR   Pfam; PF00860; Xan_ur_permease; 1.
DR   TIGRFAMs; TIGR00801; ncs2; 1.
DR   PROSITE; PS01116; XANTH_URACIL_PERMASE; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Membrane; Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..421
FT                   /note="Uracil permease"
FT                   /id="PRO_0000446238"
FT   TRANSMEM        18..38
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        41..61
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        65..85
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        89..109
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        115..135
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        160..180
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        186..206
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        232..252
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        304..324
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        329..349
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        371..391
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        393..413
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   421 AA;  44684 MW;  1721DBB9F2A93DBD CRC64;
     MKDRVIQVDE RLPFLQSIPL SLQHLFAMFG STVLVPMLLQ INPAICLLMN GIGTLIYIFL
     CKGRIPAYLG SSFAFISPVL IVISTRSYEA ALSGFLVVGL VFCLIGLLVK AVGTGWIEIV
     FPPAAMGAIV AVIGLELAPT AANMAGFVAS AGTEGWSPDP KVIAVSLVTL LTAVVGNVMF
     RGFMKIIPIL ISIIVGYALA AFLGIVDFSI VREAKWFDLP TFYIMKWDWS SIAIIVPAAL
     VVVAEHIGHL IVTSNIVGKD LSKDPGLDRS LLGNGVSTVI SSFVGSTPNT TYGENIGVLA
     LTRVYSIWII GGAAVMAIVL SFVGKLAALI QTIPVPVMGG VSILLFGVIA GSGVRMLVEA
     KVDYSNPKNL ILTAVVLIIG ISGAAFKWGN FEMKGMALAT VIAILLGLFF NIIDKLKWSN
     E