ID   URB2_DANRE              Reviewed;        1495 AA.
AC   B0V0U5;
DT   23-FEB-2022, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Unhealthy ribosome biogenesis protein 2 homolog;
GN   Name=urb2;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [2]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=29470984; DOI=10.1016/j.bbrc.2018.02.153;
RA   Cai P., Mao X., Zhao J., Luo L.;
RT   "Ribosome biogenesis protein Urb2 regulates hematopoietic stem cells
RT   development via P53 pathway in zebrafish.";
RL   Biochem. Biophys. Res. Commun. 497:776-782(2018).
CC   -!- FUNCTION: Essential for hematopietic stem cell development through the
CC       regulation of p53/TP53 pathway. {ECO:0000269|PubMed:29470984}.
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus
CC       {ECO:0000250|UniProtKB:Q14146}.
CC   -!- DEVELOPMENTAL STAGE: Expression in caudal hematopoietic tissue
CC       initiates during hematopietic stem cell expansion stages, and the
CC       expression level from 72 hours post-fertilization (hpf) to 96 hpf is
CC       much higher than that at 48 hpf. {ECO:0000269|PubMed:29470984}.
CC   -!- DISRUPTION PHENOTYPE: Mutants show a notable decrease in the population
CC       of hematopietic stem cells in caudal hematopoietic tissue and early T-
CC       cells in thymus (PubMed:29470984). Compromised cell proliferation and
CC       excessive apoptosis are observed in the caudal hematopoietic tissue
CC       (PubMed:29470984). {ECO:0000269|PubMed:29470984}.
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DR   EMBL; CR762497; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_001116773.2; NM_001123301.2.
DR   RefSeq; XP_005156935.1; XM_005156878.3.
DR   STRING; 7955.ENSDARP00000121235; -.
DR   PaxDb; B0V0U5; -.
DR   PeptideAtlas; B0V0U5; -.
DR   Ensembl; ENSDART00000138747; ENSDARP00000121235; ENSDARG00000003217.
DR   GeneID; 570267; -.
DR   KEGG; dre:570267; -.
DR   CTD; 9816; -.
DR   ZFIN; ZDB-GENE-030131-2621; urb2.
DR   eggNOG; ENOG502QWBH; Eukaryota.
DR   GeneTree; ENSGT00390000009258; -.
DR   HOGENOM; CLU_251606_0_0_1; -.
DR   InParanoid; B0V0U5; -.
DR   OMA; CYLSVQR; -.
DR   OrthoDB; 727255at2759; -.
DR   PhylomeDB; B0V0U5; -.
DR   TreeFam; TF328770; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 13.
DR   Bgee; ENSDARG00000003217; Expressed in early embryo and 24 other tissues.
DR   ExpressionAtlas; B0V0U5; baseline.
DR   GO; GO:0005730; C:nucleolus; IBA:GO_Central.
DR   GO; GO:1902036; P:regulation of hematopoietic stem cell differentiation; IMP:ZFIN.
DR   GO; GO:1901796; P:regulation of signal transduction by p53 class mediator; IMP:UniProtKB.
DR   GO; GO:0042254; P:ribosome biogenesis; IBA:GO_Central.
DR   InterPro; IPR018849; Urb2/Npa2_C.
DR   Pfam; PF10441; Urb2; 1.
PE   2: Evidence at transcript level;
KW   Nucleus; Reference proteome.
FT   CHAIN           1..1495
FT                   /note="Unhealthy ribosome biogenesis protein 2 homolog"
FT                   /id="PRO_0000454641"
SQ   SEQUENCE   1495 AA;  168788 MW;  E2DDA6ECB41EB01F CRC64;
     MAAIYSGIQL KLKNTRTPWP DKLKLARFAW ISTQCLLPNK EQVLFDWTNH ALTCFYNKKV
     EMPPEVVEGL WTYLDDILHS RKLHNVLSQG KTISLRLTVA QIINDRILDY TSGLRSVSFH
     TILSCCHGIL TSPVLSVIYT AKYELLVQLL SRLCGLTSMQ LRQQKYEEPL SLKAFEVLLV
     VLSNYLTVQK QQANSNRVFF QVTAHLLHPL LLLRHLLNTR VWTEKDDVKI RQNLSKEIRT
     KVDAVLQSAL FLSDHLQSYK VEVSPSENEA GDRKSHIGKG LQGPLNTILT RICIHGASEE
     EEALFYAVKS NSLSLLFKFA LDSFCRGGEN KRVIFHLMAK LITALGFTDE LDIDENFRAS
     NWGICLLALE NLLNSCLVGD IYNVAADRIQ HGEVQFNFYR KVARLLFNNT QMGVPAWYRC
     LRALLALNHQ ILEPDLDELL SAVWVDAENM ELRVRKAREA LVSAVLQTYS KLRQLPKLIE
     ELLDVVCRPA ADELRPALLP EAIQKTLSQC LLDNPLRQNL EICGLILKRV QSCLLPHIQD
     ETSDLALKVF SMSLLLHAVL FSFKTLDNST PVPVVKQTLS LMTEMLKVVE DLLEQKLATK
     GPWMEKVQEV TLLLAHTWVE ADALFQIHCT KYTSPSASQS STLVDKALML RNMDSPLGKL
     LQNLLALHKL KIHLLKSFSE SSEMQKMAQF ILNREELSVI HSLDQMWDFQ FCNVNSSTYN
     AAHWFLVTSN LPLIAPYLDP NDRSVFAECM LKSLLQSLDA SGSNLENTGI SVCLISRQLL
     ESPVLCELPE IFSALTKCII KALFGLLDSS HMQLICPSFL TPPVENVEVK DEEMGMTPSM
     KRLKAIGLEM LYSVKMKSSV PLSKRQINGL LQLVKVTSVL NGYAMSYEDY LELFLTLLTL
     TSTIQCVEET NLSAFIELLK ELFSVMASLL LAKNSQSILK VIHGSNLLEA TMTTLFSRSS
     EGLFKSLDGP TWLSFLQSVQ DFIQSLIRLI IDRKSSICLN LEKFTSFMVE CNFTARVLTV
     DTGDLFSLQL HLVTLSALCR EMMTTLGKNK QLDQALTHLL EKATAVMEPA IQAVLMGKGS
     CLLRQSFSVE VVTVMIRCEL ARASSSNDDG QEKISSMPFY RSFYQQILKE LFPSPRPMDF
     LISSIHYLSA FHMAAEKTKV ADLEDLHIEI LQSIQALLSG TWMSLTDVKE LEGPVKELLN
     QLMSNCSQEQ FHLFLLMLRE GLVAAKVEGG QCSEVLSAVT LIKLLACCLF PEHCSKAFWL
     ITPQIISSLI FIIKESSKLP SLTRVLTVPG LEALTVLLRQ GKAQISNPHQ VIVVLGALQF
     VPLDSHCMED YHSAFEAVHE ALFAIIHCYP QVMLKASPTF LNCFYRLVSS VMHEGKQRSD
     TDRASEKDRE SLLKCARLVE RMYTHVASAA EDFTVLSSFM VAQYVSELQR VTLQPEIKAH
     LTEGIYCILD HCVEQDIKFL NTTLQMGVKE VFNELYSSYT HYHKSQRQGE EKYTV