ID   URB2_YEAST              Reviewed;        1174 AA.
AC   P47108; D6VWL2;
DT   01-FEB-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1996, sequence version 1.
DT   03-AUG-2022, entry version 148.
DE   RecName: Full=Nucleolar pre-ribosomal-associated protein 2;
DE   AltName: Full=Unhealthy ribosome biogenesis protein 2;
GN   Name=URB2; Synonyms=NPA2; OrderedLocusNames=YJR041C; ORFNames=J1622;
OS   Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX   NCBI_TaxID=559292;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=7668047; DOI=10.1002/yea.320110809;
RA   Huang M.-E., Chuat J.-C., Galibert F.;
RT   "Analysis of a 42.5 kb DNA sequence of chromosome X reveals three tRNA
RT   genes and 14 new open reading frames including a gene most probably
RT   belonging to the family of ubiquitin-protein ligases.";
RL   Yeast 11:775-781(1995).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=8641269; DOI=10.1002/j.1460-2075.1996.tb00557.x;
RA   Galibert F., Alexandraki D., Baur A., Boles E., Chalwatzis N., Chuat J.-C.,
RA   Coster F., Cziepluch C., de Haan M., Domdey H., Durand P., Entian K.-D.,
RA   Gatius M., Goffeau A., Grivell L.A., Hennemann A., Herbert C.J.,
RA   Heumann K., Hilger F., Hollenberg C.P., Huang M.-E., Jacq C.,
RA   Jauniaux J.-C., Katsoulou C., Kirchrath L., Kleine K., Kordes E.,
RA   Koetter P., Liebl S., Louis E.J., Manus V., Mewes H.-W., Miosga T.,
RA   Obermaier B., Perea J., Pohl T.M., Portetelle D., Pujol A., Purnelle B.,
RA   Ramezani Rad M., Rasmussen S.W., Rose M., Rossau R.,
RA   Schaaff-Gerstenschlaeger I., Smits P.H.M., Scarcez T., Soriano N.,
RA   To Van D., Tzermia M., Van Broekhoven A., Vandenbol M., Wedler H.,
RA   von Wettstein D., Wambutt R., Zagulski M., Zollner A., Karpfinger-Hartl L.;
RT   "Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.";
RL   EMBO J. 15:2031-2049(1996).
RN   [3]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 204508 / S288c;
RX   PubMed=24374639; DOI=10.1534/g3.113.008995;
RA   Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA   Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA   Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT   "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL   G3 (Bethesda) 4:389-398(2014).
RN   [4]
RP   SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
RX   PubMed=14562095; DOI=10.1038/nature02026;
RA   Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W.,
RA   Weissman J.S., O'Shea E.K.;
RT   "Global analysis of protein localization in budding yeast.";
RL   Nature 425:686-691(2003).
RN   [5]
RP   LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX   PubMed=14562106; DOI=10.1038/nature02046;
RA   Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA   O'Shea E.K., Weissman J.S.;
RT   "Global analysis of protein expression in yeast.";
RL   Nature 425:737-741(2003).
RN   [6]
RP   FUNCTION, AND INTERACTION WITH URB1.
RX   PubMed=15242642; DOI=10.1016/j.cell.2004.06.013;
RA   Mnaimneh S., Davierwala A.P., Haynes J., Moffat J., Peng W.-T., Zhang W.,
RA   Yang X., Pootoolal J., Chua G., Lopez A., Trochesset M., Morse D.,
RA   Krogan N.J., Hiley S.L., Li Z., Morris Q., Grigull J., Mitsakakis N.,
RA   Roberts C.J., Greenblatt J.F., Boone C., Kaiser C.A., Andrews B.J.,
RA   Hughes T.R.;
RT   "Exploration of essential gene functions via titratable promoter alleles.";
RL   Cell 118:31-44(2004).
RN   [7]
RP   IDENTIFICATION BY MASS SPECTROMETRY, INTERACTION WITH URB1, AND SUBCELLULAR
RP   LOCATION.
RX   PubMed=15226434; DOI=10.1128/mcb.24.14.6324-6337.2004;
RA   Dez C., Froment C., Noaillac-Depeyre J., Monsarrat B.,
RA   Caizergues-Ferrer M., Henry Y.;
RT   "Npa1p, a component of very early pre-60S ribosomal particles, associates
RT   with a subset of small nucleolar RNPs required for peptidyl transferase
RT   center modification.";
RL   Mol. Cell. Biol. 24:6324-6337(2004).
RN   [8]
RP   FUNCTION, SUBUNIT, IDENTIFICATION IN A COMPLEX WITH DBP6; NOP8; URB1 AND
RP   RSA3, AND SUBCELLULAR LOCATION.
RX   PubMed=17145778; DOI=10.1128/mcb.01523-06;
RA   Rosado I.V., Dez C., Lebaron S., Caizergues-Ferrer M., Henry Y.,
RA   de la Cruz J.;
RT   "Characterization of Saccharomyces cerevisiae Npa2p (Urb2p) reveals a Low-
RT   molecular-mass complex containing Dbp6p, Npa1p (Urb1p), Nop8p, and Rsa3p
RT   involved in early steps of 60S ribosomal subunit biogenesis.";
RL   Mol. Cell. Biol. 27:1207-1221(2007).
CC   -!- FUNCTION: Required for 27S pre-rRNA processing. Has an early role
CC       during 60S ribosomal subunit assembly. {ECO:0000269|PubMed:15242642,
CC       ECO:0000269|PubMed:17145778}.
CC   -!- SUBUNIT: Component of very early pre-60S ribosomal particles containing
CC       27SA2 pre-rRNA. Interacts with URB1. Together with DBP6, NOP8, URB1 and
CC       RSA3, forms an RNA-independent complex, which is required during early
CC       maturation of nascent 60S ribosomal subunits.
CC       {ECO:0000269|PubMed:15226434, ECO:0000269|PubMed:15242642,
CC       ECO:0000269|PubMed:17145778}.
CC   -!- INTERACTION:
CC       P47108; P53734: DBP6; NbExp=4; IntAct=EBI-25492, EBI-5625;
CC       P47108; Q05942: RSA3; NbExp=5; IntAct=EBI-25492, EBI-33602;
CC       P47108; P34241: URB1; NbExp=5; IntAct=EBI-25492, EBI-26595;
CC   -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:14562095,
CC       ECO:0000269|PubMed:15226434, ECO:0000269|PubMed:17145778}.
CC   -!- MISCELLANEOUS: Present with 1660 molecules/cell in log phase SD medium.
CC       {ECO:0000269|PubMed:14562106}.
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DR   EMBL; L36344; AAA88743.1; -; Genomic_DNA.
DR   EMBL; Z49541; CAA89568.1; -; Genomic_DNA.
DR   EMBL; Z49542; CAA89570.1; -; Genomic_DNA.
DR   EMBL; BK006943; DAA08828.1; -; Genomic_DNA.
DR   PIR; S57060; S57060.
DR   RefSeq; NP_012575.3; NM_001181699.3.
DR   AlphaFoldDB; P47108; -.
DR   BioGRID; 33792; 179.
DR   ComplexPortal; CPX-1421; NOP8 60s ribosome pre-assembly complex.
DR   DIP; DIP-6348N; -.
DR   IntAct; P47108; 9.
DR   MINT; P47108; -.
DR   STRING; 4932.YJR041C; -.
DR   iPTMnet; P47108; -.
DR   MaxQB; P47108; -.
DR   PaxDb; P47108; -.
DR   PRIDE; P47108; -.
DR   EnsemblFungi; YJR041C_mRNA; YJR041C; YJR041C.
DR   GeneID; 853498; -.
DR   KEGG; sce:YJR041C; -.
DR   SGD; S000003802; URB2.
DR   VEuPathDB; FungiDB:YJR041C; -.
DR   eggNOG; ENOG502QTEB; Eukaryota.
DR   HOGENOM; CLU_008472_0_0_1; -.
DR   InParanoid; P47108; -.
DR   OMA; CHVNARE; -.
DR   BioCyc; YEAST:G3O-31676-MON; -.
DR   PRO; PR:P47108; -.
DR   Proteomes; UP000002311; Chromosome X.
DR   RNAct; P47108; protein.
DR   GO; GO:0005730; C:nucleolus; IDA:SGD.
DR   GO; GO:0005634; C:nucleus; IC:ComplexPortal.
DR   GO; GO:0042273; P:ribosomal large subunit biogenesis; IC:ComplexPortal.
DR   GO; GO:0042254; P:ribosome biogenesis; IPI:SGD.
DR   GO; GO:0016072; P:rRNA metabolic process; IMP:SGD.
DR   GO; GO:0006364; P:rRNA processing; IEA:UniProtKB-KW.
DR   InterPro; IPR018849; Urb2/Npa2_C.
DR   Pfam; PF10441; Urb2; 1.
PE   1: Evidence at protein level;
KW   Nucleus; Reference proteome; Ribosome biogenesis; rRNA processing.
FT   CHAIN           1..1174
FT                   /note="Nucleolar pre-ribosomal-associated protein 2"
FT                   /id="PRO_0000203094"
SQ   SEQUENCE   1174 AA;  135118 MW;  7647D48DA557D4D4 CRC64;
     MGDLTEELSI PDNAQDLSKL LRSTSTKPHQ IAEIVSKFDK LETYFPKKEI FVLDLLIDRL
     NNGNLDDFKT SEHTWIIFTR LLDAINDPIS IKKLLKKLKT VPVMIRTFFL WPKDKLLTRS
     VSFIKAFFAI NDYLIVNFSV EESFQLLEHA INGLSSCPTT DFALSYLQDA CNLTHVDNIT
     TTDNKIATCY CKHMLLPSLR YFAQTKNSAS SNQSFIRLSH FMGKFLLQPR IDYMKLNKKF
     VQENASEITD DMAYYYFATF VTFLSKDNFA QLEVIFTILG AKKPSLECRF LNLLSESKKT
     VSQEFLEALL LEMLASTDES GVLSLIPIIL KLDIEVAIKH IFRLLELIQL ENLNDPLFSS
     HIWDLIIQSH ANARELSDFF AKINEYCSRK GPDSYFLINH PAYVKSITKQ LFTLSSLQWK
     NLLQALLDQV NHDSTNRVPL YLIRICLEGL SEGASRATLD EVKPILSQVF TLESFNNSLQ
     WDLKYHIMEV YDDIVPAEEL EKIDYVLSSN IFDTTSADVE ELFFYCFKLR EYISFDLSDA
     KKKFMRHFEI LDEERKSNLS YSVVSKFATL VNNNFTREQI SSLIDSLLLN STNLSSLLKN
     DDIFEETNIT YALINKLASS YHQTFALEAL IQIPIQCINK NVRVALINNL TCESFCLDSA
     TRECLLHLLS SPTFKSNIET NFYELCEKTI MSPEMAISET GDEKKEIEDK ISIFEKVWTN
     HLSQAKEPVS EKFLESGYDI VKQSMSLSNG DSKLIIAGFT IAKFLKPDNK HRDIQGMAIS
     YAVKILENYS ENFESETIPL FRISMSTLYK IITTGQGDIS KHKSRILDIF SKIMLRYHSK
     KVYHAPEEQE MFLVHSLLTE NKLEYIFAEY LNIEHTDKCD SALGFCLEES LKQGPDAFNR
     LLWNSAKSFS TISQPCAEKF VRVFIIMSKR IARDNNLGHH LFVIALLEAY TYCDIEKFGY
     KSYLLLFNAI KEFLVSKPWL FSQYCIEMLL PFCLKTLAFI VNHESTDEIN EGFINIIEVI
     DHMLLVHRFK FSNRHHLFNS VLCQILEIIA IHDGTLCANS ADAVARLITN YCEPYNVSNA
     QNGQKNNLSS KISLIKQSIR KNVLVVLTKY IQLSITTQFS LNIKKSLQPG IHAIFDILSQ
     NELNQLNAFL DTPGKQYFKA LYLQYKKVGK WRED