ID   URE1_ECOLX              Reviewed;          30 AA.
AC   Q03284;
DT   01-OCT-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1993, sequence version 1.
DT   25-MAY-2022, entry version 88.
DE   RecName: Full=Urease subunit alpha;
DE            EC=3.5.1.5;
DE   AltName: Full=Urea amidohydrolase subunit alpha;
DE   Flags: Fragment;
GN   Name=ureC;
OS   Escherichia coli.
OG   Plasmid.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=562;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=1440 / UPEC;
RX   PubMed=8449894; DOI=10.1128/jb.175.6.1860-1864.1993;
RA   D'Orazio S.E., Collins C.M.;
RT   "Characterization of a plasmid-encoded urease gene cluster found in members
RT   of the family Enterobacteriaceae.";
RL   J. Bacteriol. 175:1860-1864(1993).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
CC   -!- COFACTOR:
CC       Name=Ni cation; Xref=ChEBI:CHEBI:25516; Evidence={ECO:0000305};
CC       Note=Binds 2 nickel ions per subunit. {ECO:0000305};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from
CC       urea (urease route): step 1/1.
CC   -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha)
CC       subunits. Three heterotrimers associate to form the active enzyme (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- INDUCTION: The probable operon is induced by urea.
CC       {ECO:0000269|PubMed:8449894}.
CC   -!- SIMILARITY: Belongs to the metallo-dependent hydrolases superfamily.
CC       Urease alpha subunit family. {ECO:0000305}.
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DR   EMBL; L03307; AAA24747.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q03284; -.
DR   SMR; Q03284; -.
DR   ChEMBL; CHEMBL2364683; -.
DR   DrugCentral; Q03284; -.
DR   UniPathway; UPA00258; UER00370.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-EC.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   Gene3D; 2.30.40.10; -; 1.
DR   InterPro; IPR011059; Metal-dep_hydrolase_composite.
DR   InterPro; IPR011612; Urease_alpha_N_dom.
DR   Pfam; PF00449; Urease_alpha; 1.
DR   SUPFAM; SSF51338; SSF51338; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasm; Hydrolase; Metal-binding; Nickel; Plasmid.
FT   CHAIN           1..>30
FT                   /note="Urease subunit alpha"
FT                   /id="PRO_0000067541"
FT   NON_TER         30
SQ   SEQUENCE   30 AA;  3419 MW;  C771DFA3273BAA96 CRC64;
     MKTISRQAYA DMFGPTTGDR LRLADTELFL