URE22_BRUSU
ID URE22_BRUSU Reviewed; 159 AA.
AC Q8FZW3; G0KB42;
DT 02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 99.
DE RecName: Full=Urease subunit beta 2 {ECO:0000255|HAMAP-Rule:MF_01954};
DE EC=3.5.1.5 {ECO:0000255|HAMAP-Rule:MF_01954};
DE AltName: Full=Urea amidohydrolase subunit beta 2 {ECO:0000255|HAMAP-Rule:MF_01954};
GN Name=ureB2 {ECO:0000255|HAMAP-Rule:MF_01954};
GN OrderedLocusNames=BR1357, BS1330_I1352;
OS Brucella suis biovar 1 (strain 1330).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=204722;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=12271122; DOI=10.1073/pnas.192319099;
RA Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT "The Brucella suis genome reveals fundamental similarities between animal
RT and plant pathogens and symbionts.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=22038969; DOI=10.1128/jb.06181-11;
RA Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT "Revised genome sequence of Brucella suis 1330.";
RL J. Bacteriol. 193:6410-6410(2011).
RN [3]
RP OPERON DISRUPTION, AND LACK OF ROLE IN VIRULENCE.
RC STRAIN=1330;
RX PubMed=17578575; DOI=10.1186/1471-2180-7-57;
RA Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M.,
RA Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N.,
RA Schurig G.G., Boyle S.M.;
RT "Brucella suis urease encoded by ure1 but not ure2 is necessary for
RT intestinal infection of BALB/c mice.";
RL BMC Microbiol. 7:57-57(2007).
CC -!- FUNCTION: Disrupting the ure2 operon has no effect on urease activity,
CC or pathogen survival in BALB/c mice when inoculated by gavage, but
CC confers slightly enhanced resistance to low pH killing in vitro.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_01954};
CC -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from
CC urea (urease route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_01954}.
CC -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha)
CC subunits. Three heterotrimers associate to form the active enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_01954}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01954}.
CC -!- SIMILARITY: Belongs to the urease beta subunit family.
CC {ECO:0000255|HAMAP-Rule:MF_01954}.
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DR EMBL; AE014291; AAN30271.1; -; Genomic_DNA.
DR EMBL; CP002997; AEM18688.1; -; Genomic_DNA.
DR RefSeq; WP_004688520.1; NZ_KN046804.1.
DR AlphaFoldDB; Q8FZW3; -.
DR SMR; Q8FZW3; -.
DR EnsemblBacteria; AEM18688; AEM18688; BS1330_I1352.
DR GeneID; 45052377; -.
DR GeneID; 55591017; -.
DR KEGG; bms:BR1357; -.
DR KEGG; bsi:BS1330_I1352; -.
DR PATRIC; fig|204722.21.peg.831; -.
DR HOGENOM; CLU_129707_2_0_5; -.
DR OMA; VVHNTGD; -.
DR PhylomeDB; Q8FZW3; -.
DR BRENDA; 3.5.1.5; 14929.
DR UniPathway; UPA00258; UER00370.
DR Proteomes; UP000007104; Chromosome I.
DR GO; GO:0035550; C:urease complex; IEA:InterPro.
DR GO; GO:0009039; F:urease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00407; Urease_beta; 1.
DR Gene3D; 2.10.150.10; -; 1.
DR HAMAP; MF_01954; Urease_beta; 1.
DR InterPro; IPR002019; Urease_beta.
DR InterPro; IPR036461; Urease_betasu_sf.
DR Pfam; PF00699; Urease_beta; 1.
DR SUPFAM; SSF51278; SSF51278; 1.
DR TIGRFAMs; TIGR00192; urease_beta; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Hydrolase.
FT CHAIN 1..159
FT /note="Urease subunit beta 2"
FT /id="PRO_0000234239"
FT REGION 1..24
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 159 AA; 17762 MW; F46CB772A415D1F6 CRC64;
MAKEPTKAAH PQPEQTKTNH KAHRPVGGYV LAKDPIEINQ GRPRTTLTVR NTGDRPIQIG
SHFHFFEVNR YLEFDRSKAF GLRLDIPANT AVRFEPGDEK EVTLVPFAGK RFIFGFNNLV
DGWSGDGPTP DYQPNREIAA ERAEKLGFKS CKSGGKDAK
