CAC1M_MUSDO
ID CAC1M_MUSDO Reviewed; 1687 AA.
AC Q25452;
DT 15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Muscle calcium channel subunit alpha-1;
DE AltName: Full=MDL-alpha-1;
OS Musca domestica (House fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Muscoidea;
OC Muscidae; Musca.
OX NCBI_TaxID=7370;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Muscle;
RX PubMed=8313972; DOI=10.1016/0014-5793(94)80413-3;
RA Grabner M., Bachmann A., Rosenthal F., Striessnig J., Schultz C., Tautz D.,
RA Glossmann H.;
RT "Insect calcium channels. Molecular cloning of an alpha 1-subunit from
RT housefly (Musca domestica) muscle.";
RL FEBS Lett. 339:189-194(1994).
CC -!- FUNCTION: Voltage-sensitive calcium channels (VSCC) mediate the entry
CC of calcium ions into excitable cells and are also involved in a variety
CC of calcium-dependent processes, including muscle contraction, hormone
CC or neurotransmitter release, gene expression, cell motility, cell
CC division and cell death (By similarity). MDL-alpha1 encodes a
CC dihydropyridine- and diltiazem-sensitive current in larval body wall
CC muscle. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Predominantly expressed in the larval body wall
CC musculature. In adults, highest expression in thorax followed by head
CC and at a lower extent by abdomen.
CC -!- DEVELOPMENTAL STAGE: Late embryonic.
CC -!- DOMAIN: Each of the four internal repeats contains five hydrophobic
CC transmembrane segments (S1, S2, S3, S5, S6) and one positively charged
CC transmembrane segment (S4). S4 segments probably represent the voltage-
CC sensor and are characterized by a series of positively charged amino
CC acids at every third position.
CC -!- SIMILARITY: Belongs to the calcium channel alpha-1 subunit (TC
CC 1.A.1.11) family. {ECO:0000305}.
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DR EMBL; Z31723; CAA83514.1; -; mRNA.
DR PIR; S41742; S41742.
DR AlphaFoldDB; Q25452; -.
DR SMR; Q25452; -.
DR STRING; 7370.XP_005191382.1; -.
DR VEuPathDB; VectorBase:MDOA002080; -.
DR VEuPathDB; VectorBase:MDOA005409; -.
DR VEuPathDB; VectorBase:MDOA010384; -.
DR VEuPathDB; VectorBase:MDOA014412; -.
DR eggNOG; KOG0017; Eukaryota.
DR eggNOG; KOG2301; Eukaryota.
DR Proteomes; UP000095301; Whole Genome Shotgun Assembly.
DR GO; GO:0005891; C:voltage-gated calcium channel complex; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005245; F:voltage-gated calcium channel activity; IEA:InterPro.
DR GO; GO:0034765; P:regulation of ion transmembrane transport; IEA:UniProtKB-KW.
DR Gene3D; 1.20.120.350; -; 4.
DR InterPro; IPR031649; GPHH_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR014873; VDCC_a1su_IQ.
DR InterPro; IPR005446; VDCC_L_a1su.
DR InterPro; IPR002077; VDCCAlpha1.
DR InterPro; IPR043203; VGCC_Ca_Na.
DR InterPro; IPR027359; Volt_channel_dom_sf.
DR PANTHER; PTHR10037; PTHR10037; 2.
DR Pfam; PF08763; Ca_chan_IQ; 1.
DR Pfam; PF16905; GPHH; 1.
DR Pfam; PF00520; Ion_trans; 4.
DR PRINTS; PR00167; CACHANNEL.
DR PRINTS; PR01630; LVDCCALPHA1.
DR SMART; SM01062; Ca_chan_IQ; 1.
PE 2: Evidence at transcript level;
KW Calcium; Calcium channel; Calcium transport; Glycoprotein; Ion channel;
KW Ion transport; Membrane; Metal-binding; Phosphoprotein; Repeat;
KW Transmembrane; Transmembrane helix; Transport; Voltage-gated channel.
FT CHAIN 1..1687
FT /note="Muscle calcium channel subunit alpha-1"
FT /id="PRO_0000053961"
FT TOPO_DOM 1..61
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 62..80
FT /note="Helical; Name=S1 of repeat I"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..99
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 100..117
FT /note="Helical; Name=S2 of repeat I"
FT /evidence="ECO:0000255"
FT TOPO_DOM 118..130
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 131..145
FT /note="Helical; Name=S3 of repeat I"
FT /evidence="ECO:0000255"
FT TOPO_DOM 146..157
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 158..176
FT /note="Helical; Name=S4 of repeat I"
FT /evidence="ECO:0000255"
FT TOPO_DOM 177..196
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 197..216
FT /note="Helical; Name=S5 of repeat I"
FT /evidence="ECO:0000255"
FT TOPO_DOM 217..302
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 303..327
FT /note="Helical; Name=S6 of repeat I"
FT /evidence="ECO:0000255"
FT TOPO_DOM 328..434
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 435..454
FT /note="Helical; Name=S1 of repeat II"
FT /evidence="ECO:0000255"
FT TOPO_DOM 455..467
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 468..487
FT /note="Helical; Name=S2 of repeat II"
FT /evidence="ECO:0000255"
FT TOPO_DOM 488..496
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 497..515
FT /note="Helical; Name=S3 of repeat II"
FT /evidence="ECO:0000255"
FT TOPO_DOM 516..525
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 526..544
FT /note="Helical; Name=S4 of repeat II"
FT /evidence="ECO:0000255"
FT TOPO_DOM 545..563
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 564..583
FT /note="Helical; Name=S5 of repeat II"
FT /evidence="ECO:0000255"
FT TOPO_DOM 584..639
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 640..664
FT /note="Helical; Name=S6 of repeat II"
FT /evidence="ECO:0000255"
FT TOPO_DOM 665..785
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 786..809
FT /note="Helical; Name=S1 of repeat III"
FT /evidence="ECO:0000255"
FT TOPO_DOM 810..826
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 827..846
FT /note="Helical; Name=S2 of repeat III"
FT /evidence="ECO:0000255"
FT TOPO_DOM 847..854
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 855..877
FT /note="Helical; Name=S3 of repeat III"
FT /evidence="ECO:0000255"
FT TOPO_DOM 878..885
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 886..900
FT /note="Helical; Name=S4 of repeat III"
FT /evidence="ECO:0000255"
FT TOPO_DOM 901..921
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 922..941
FT /note="Helical; Name=S5 of repeat III"
FT /evidence="ECO:0000255"
FT TOPO_DOM 942..1030
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1031..1055
FT /note="Helical; Name=S6 of repeat III"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1056..1110
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1111..1129
FT /note="Helical; Name=S1 of repeat IV"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1130..1143
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1144..1163
FT /note="Helical; Name=S2 of repeat IV"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1164..1172
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1173..1191
FT /note="Helical; Name=S3 of repeat IV"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1192..1219
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1220..1238
FT /note="Helical; Name=S4 of repeat IV"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1239..1257
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 1258..1277
FT /note="Helical; Name=S5 of repeat IV"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1278..1343
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 1344..1362
FT /note="Helical; Name=S6 of repeat IV"
FT /evidence="ECO:0000255"
FT TOPO_DOM 1363..1687
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT REPEAT 48..330
FT /note="I"
FT REPEAT 420..667
FT /note="II"
FT REPEAT 777..1059
FT /note="III"
FT REPEAT 1096..1370
FT /note="IV"
FT REGION 1..33
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 979..1068
FT /note="Dihydropyridine binding"
FT /evidence="ECO:0000250"
FT REGION 1327..1389
FT /note="Dihydropyridine binding"
FT /evidence="ECO:0000250"
FT REGION 1337..1378
FT /note="Phenylalkylamine binding"
FT /evidence="ECO:0000250"
FT BINDING 285
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P07293"
FT BINDING 617
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P07293"
FT BINDING 1005
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000250|UniProtKB:P07293"
FT CARBOHYD 90
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1687 AA; 193875 MW; 628606AFE46DDDEF CRC64;
MDDAVCPTET DNVQNKQKAT TPKRTQRRGG KQQLDRPERA LFCLTLKNPL RIFCIKIVDS
KLFEYFILLT IFANCVALAV YTPYPSGDSN ITNQMLEKIE YIFLVIFTSE CVMKIIAYGF
VLHTGSYLRN GWNFLDFFIV VIGMISTALS NLVKEGFDVK ALRAFRVLRP LRLVSGVPSL
QVVLNSILKA MIPLLHIALL VLFVIIIYAI IGLELFSGKL HKTCRHSNTG EYLNDLDELH
ACGVGFKCPS GYECFDDWVG PNDGITNFDN FGLSMLTVFQ CITLEGWTDV LYSIQDAMGS
SWEWIYFVSM VILGAFFVMN LILGVLSGEF SKERTKAKNR GDFQKLREKQ QIEEDLRGYL
DWITQAEDIE PDPDAQIIED CHKNKVKEVV SIDNLKDHEN ETQQTDSWFR SQKKYLERIN
RRIRRACRKA VKSQAFYWLI ILLVFLNTGV LATEHYRQPI WLDQFQEYTN IFFIALFTCE
MILKMYSLGF QGYFVSLFNR FDCFVVIGSI SEMVLTSSEL MAPLGVSVLR CVRLLRVFKV
TKYWHSLSNL VASLLNSIQS IASLLLLLFL FIVIFGLLGM QVFGGRFTFK PEEEKPRSNF
DSFYQSLLTV FQILTGEDWN VVMYDGIRAY GGVFSFGIVA CIYYIILFIC GNYILLNVFL
AIAVDNLADA DSLSTIEKED ESQIQLDNQI KNEMENEEYL QNGDHISFKA EFGADLDTYL
QDEECGSYSD DENTYNKLGG VKQRVSSLPR RNTNTDMDRI KKDIPYGTSF FIFSHTNRFR
IFCHRLCNHS NFGNFILCCI MFSSAMLAAE NPLKADASRN IVLNKFDYFF TAVFTIELVL
KLISYGFVLH DGAFCRSAFN LLDLLVVCVS LISIFFNSNA ISVVKILRVL RVLRPLRAIN
RAKGLKHVVQ CVIVAVKTIG NIVLVTCLLQ FMFAVIGVQL FKGKFFSCSD GSKVYESDCH
GTYLFYENGD INKPRLKERE WKNNKFHFDD VAKAMLTLFT VSTFEGWPTL LYVSIDSNKE
NGGPIYNFRP IVAAYYIIYI IIIAFFMVNI FVGFVIVTFQ NEGEQEYKNC ELDKNQRNCI
EFALKAKPVR RYIPKHSIQY KVWWFVTSSS FEYSIFVLIM INTVTLAMKF YKQPEYYSEI
LDALNMIFTA VFSLEFIFKL AAFRFKNYFG DAWNTFDFII VLGSFIDIVY SEIKTKEQAL
ATCDGQSCNK AKGGSTLISI NFFRLFRVMR LVKLLSKGEG IRTLLWTFIK SFQALPYVAL
LIVMLFFIYA VIGMQVFGKI MLEEGTSIDR NNNFQTFPQA VLVLFRSATG EAWQEIMMAC
SPRDDVKCDP ESDAVNNCGS SIAFPYFISF YVLCSFLIIN LFVAVIMDNF DYLTRDWSIL
GPHHLDEFIR LWSEYDPDAK GRIKHLDVVT LLRKISPPLG FGKLCPHRMA CKRLVSMNMP
LNSDGTVLFN ATLFAVVRLP LAIKTDGNID EANAELRATI KQIWKRTNPR LLDQVVLTGN
DDEVTVGKFY ATYLIQDYFR RFKKRKEQEG KCDQTENAVT LQAGLRTLQQ NSPALKRTIS
GYLDELASEA DPMHRRHHSL FGKVMSSLLR HEEITSSKIK HLSRTKTIPY QVEFLQHKVQ
LERNKDCLTS ASDDISIEKI IRKQNKYNGQ YFNSEANFMD NIKYTPRYDG EEYEMEDPKS
KDKDEEF
