ID   URE2_STAS1              Reviewed;         134 AA.
AC   Q4A0J4;
DT   02-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 105.
DE   RecName: Full=Urease subunit beta {ECO:0000255|HAMAP-Rule:MF_01954};
DE            EC=3.5.1.5 {ECO:0000255|HAMAP-Rule:MF_01954};
DE   AltName: Full=Urea amidohydrolase subunit beta {ECO:0000255|HAMAP-Rule:MF_01954};
GN   Name=ureB {ECO:0000255|HAMAP-Rule:MF_01954}; OrderedLocusNames=SSP0264;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-6, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL
RP   PROPERTIES.
RX   PubMed=8042901; DOI=10.1007/bf00288948;
RA   Schaefer U.K., Kaltwasser H.;
RT   "Urease from Staphylococcus saprophyticus: purification, characterization
RT   and comparison to Staphylococcus xylosus urease.";
RL   Arch. Microbiol. 161:393-399(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_01954,
CC         ECO:0000269|PubMed:8042901};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=9.5 mM for urea (at 37 degrees Celsius and pH 7.0)
CC         {ECO:0000269|PubMed:8042901};
CC         Vmax=1.979 mmol/min/mg enzyme {ECO:0000269|PubMed:8042901};
CC       pH dependence:
CC         Optimum pH is 7.0 in phosphate buffer. {ECO:0000269|PubMed:8042901};
CC       Temperature dependence:
CC         Optimum temperature is 55 degrees Celsius.
CC         {ECO:0000269|PubMed:8042901};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from
CC       urea (urease route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_01954}.
CC   -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha)
CC       subunits. Three heterotrimers associate to form the active enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_01954}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01954}.
CC   -!- SIMILARITY: Belongs to the urease beta subunit family.
CC       {ECO:0000255|HAMAP-Rule:MF_01954}.
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DR   EMBL; AP008934; BAE17409.1; -; Genomic_DNA.
DR   RefSeq; WP_011302250.1; NZ_MTGA01000037.1.
DR   AlphaFoldDB; Q4A0J4; -.
DR   SMR; Q4A0J4; -.
DR   STRING; 342451.SSP0264; -.
DR   EnsemblBacteria; BAE17409; BAE17409; SSP0264.
DR   KEGG; ssp:SSP0264; -.
DR   PATRIC; fig|342451.11.peg.267; -.
DR   eggNOG; COG0832; Bacteria.
DR   HOGENOM; CLU_129707_2_2_9; -.
DR   OMA; FYEVNDA; -.
DR   OrthoDB; 1755809at2; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0035550; C:urease complex; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00407; Urease_beta; 1.
DR   Gene3D; 2.10.150.10; -; 1.
DR   HAMAP; MF_01954; Urease_beta; 1.
DR   InterPro; IPR002019; Urease_beta.
DR   InterPro; IPR036461; Urease_betasu_sf.
DR   Pfam; PF00699; Urease_beta; 1.
DR   SUPFAM; SSF51278; SSF51278; 1.
DR   TIGRFAMs; TIGR00192; urease_beta; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Hydrolase; Reference proteome.
FT   CHAIN           1..134
FT                   /note="Urease subunit beta"
FT                   /id="PRO_0000234274"
SQ   SEQUENCE   134 AA;  14930 MW;  1416F1E2CD62E495 CRC64;
     MKPGEIIVKR TEIEVNQGHN ATILNVKNTG DRPIQVGSHY HFFEANPALQ FDHEKAYGKR
     LDIPAGAAVR FEPGDEKEVQ LVEYSGKRKI YGFHGDVNGS IDESRVYKLE DDSTATEVIA
     EQDKTSENAN KGRG