ID   URE32_BRUSU             Reviewed;         100 AA.
AC   Q8FZW4; G0KB41;
DT   28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 104.
DE   RecName: Full=Urease subunit gamma 2 {ECO:0000255|HAMAP-Rule:MF_00739};
DE            EC=3.5.1.5 {ECO:0000255|HAMAP-Rule:MF_00739};
DE   AltName: Full=Urea amidohydrolase subunit gamma 2 {ECO:0000255|HAMAP-Rule:MF_00739};
GN   Name=ureA2 {ECO:0000255|HAMAP-Rule:MF_00739}; Synonyms=ureA-2;
GN   OrderedLocusNames=BR1356, BS1330_I1351;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
RN   [3]
RP   OPERON DISRUPTION, AND LACK OF ROLE IN VIRULENCE.
RC   STRAIN=1330;
RX   PubMed=17578575; DOI=10.1186/1471-2180-7-57;
RA   Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M.,
RA   Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N.,
RA   Schurig G.G., Boyle S.M.;
RT   "Brucella suis urease encoded by ure1 but not ure2 is necessary for
RT   intestinal infection of BALB/c mice.";
RL   BMC Microbiol. 7:57-57(2007).
CC   -!- FUNCTION: Disrupting the ure2 operon has no effect on urease activity,
CC       or pathogen survival in BALB/c mice when inoculated by gavage, but
CC       confers slightly enhanced resistance to low pH killing in vitro.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00739};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from
CC       urea (urease route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00739}.
CC   -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha)
CC       subunits. Three heterotrimers associate to form the active enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00739}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00739}.
CC   -!- SIMILARITY: Belongs to the urease gamma subunit family.
CC       {ECO:0000255|HAMAP-Rule:MF_00739}.
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DR   EMBL; AE014291; AAN30270.1; -; Genomic_DNA.
DR   EMBL; CP002997; AEM18687.1; -; Genomic_DNA.
DR   PIR; AC3333; AC3333.
DR   RefSeq; WP_002964469.1; NZ_KN046804.1.
DR   AlphaFoldDB; Q8FZW4; -.
DR   SMR; Q8FZW4; -.
DR   EnsemblBacteria; AEM18687; AEM18687; BS1330_I1351.
DR   GeneID; 45052376; -.
DR   KEGG; bms:BR1356; -.
DR   KEGG; bsi:BS1330_I1351; -.
DR   PATRIC; fig|204722.21.peg.830; -.
DR   HOGENOM; CLU_145825_1_0_5; -.
DR   OMA; MNLAPRE; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000007104; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_00739; Urease_gamma; 1.
DR   InterPro; IPR012010; Urease_gamma.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   PIRSF; PIRSF001223; Urease_gamma; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Hydrolase.
FT   CHAIN           1..100
FT                   /note="Urease subunit gamma 2"
FT                   /id="PRO_0000098004"
SQ   SEQUENCE   100 AA;  10943 MW;  FABF969BACC53D05 CRC64;
     MHLTPREFDK LVIHMLSDVA LKRKNKGLKL NHPEAVAVLS AYVLDGAREG KTVEEVMDGA
     RSVLKADDVM DGVPDLLPLI QVEAVFSDGS RLVSLHNPIT