URE3_ECO57
ID URE3_ECO57 Reviewed; 100 AA.
AC Q8XAG2;
DT 28-NOV-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=Urease subunit gamma {ECO:0000255|HAMAP-Rule:MF_00739};
DE EC=3.5.1.5 {ECO:0000255|HAMAP-Rule:MF_00739};
DE AltName: Full=Urea amidohydrolase subunit gamma {ECO:0000255|HAMAP-Rule:MF_00739};
GN Name=ureA1 {ECO:0000255|HAMAP-Rule:MF_00739};
GN OrderedLocusNames=Z1143, ECs1322;
GN and
GN Name=ureA2 {ECO:0000255|HAMAP-Rule:MF_00739}; OrderedLocusNames=Z1582;
OS Escherichia coli O157:H7.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83334;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / EDL933 / ATCC 700927 / EHEC;
RX PubMed=11206551; DOI=10.1038/35054089;
RA Perna N.T., Plunkett G. III, Burland V., Mau B., Glasner J.D., Rose D.J.,
RA Mayhew G.F., Evans P.S., Gregor J., Kirkpatrick H.A., Posfai G.,
RA Hackett J., Klink S., Boutin A., Shao Y., Miller L., Grotbeck E.J.,
RA Davis N.W., Lim A., Dimalanta E.T., Potamousis K., Apodaca J.,
RA Anantharaman T.S., Lin J., Yen G., Schwartz D.C., Welch R.A.,
RA Blattner F.R.;
RT "Genome sequence of enterohaemorrhagic Escherichia coli O157:H7.";
RL Nature 409:529-533(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=11258796; DOI=10.1093/dnares/8.1.11;
RA Hayashi T., Makino K., Ohnishi M., Kurokawa K., Ishii K., Yokoyama K.,
RA Han C.-G., Ohtsubo E., Nakayama K., Murata T., Tanaka M., Tobe T., Iida T.,
RA Takami H., Honda T., Sasakawa C., Ogasawara N., Yasunaga T., Kuhara S.,
RA Shiba T., Hattori M., Shinagawa H.;
RT "Complete genome sequence of enterohemorrhagic Escherichia coli O157:H7 and
RT genomic comparison with a laboratory strain K-12.";
RL DNA Res. 8:11-22(2001).
RN [3]
RP ABSENCE OF UREASE.
RC STRAIN=O157:H7 / Sakai / RIMD 0509952 / EHEC;
RX PubMed=15470125; DOI=10.1099/mic.0.27280-0;
RA Nakano M., Iida T., Honda T.;
RT "Urease activity of enterohaemorrhagic Escherichia coli depends on a
RT specific one-base substitution in ureD.";
RL Microbiology 150:3483-3489(2004).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199,
CC ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00739};
CC -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from
CC urea (urease route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00739}.
CC -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha)
CC subunits. Three heterotrimers associate to form the active enzyme.
CC {ECO:0000255|HAMAP-Rule:MF_00739}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00739}.
CC -!- SIMILARITY: Belongs to the urease gamma subunit family.
CC {ECO:0000255|HAMAP-Rule:MF_00739}.
CC -!- CAUTION: Neither O157 strain expresses urease due to a truncation of
CC ureD, the last gene of the probable operon. Urease activity is restored
CC in O157 / Sakai upon complementation with wild-type ureD.
CC {ECO:0000305}.
CC -!- CAUTION: This region of the chromosome is duplicated in strain O157:H7
CC / EDL933 but not in O157:H7 / Sakai. {ECO:0000305}.
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DR EMBL; AE005174; AAG55288.1; -; Genomic_DNA.
DR EMBL; AE005174; AAG55697.1; -; Genomic_DNA.
DR EMBL; BA000007; BAB34745.1; -; Genomic_DNA.
DR PIR; B90794; B90794.
DR PIR; D85603; D85603.
DR RefSeq; NP_309349.1; NC_002695.1.
DR RefSeq; WP_000424145.1; NZ_SWKA01000005.1.
DR AlphaFoldDB; Q8XAG2; -.
DR SMR; Q8XAG2; -.
DR STRING; 155864.EDL933_1042; -.
DR EnsemblBacteria; AAG55288; AAG55288; Z1143.
DR EnsemblBacteria; AAG55697; AAG55697; Z1582.
DR EnsemblBacteria; BAB34745; BAB34745; ECs_1322.
DR GeneID; 913824; -.
DR KEGG; ece:Z1143; -.
DR KEGG; ece:Z1582; -.
DR KEGG; ecs:ECs_1322; -.
DR PATRIC; fig|386585.9.peg.1427; -.
DR eggNOG; COG0831; Bacteria.
DR HOGENOM; CLU_145825_1_0_6; -.
DR OMA; MQLTPHE; -.
DR UniPathway; UPA00258; UER00370.
DR Proteomes; UP000000558; Chromosome.
DR Proteomes; UP000002519; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR GO; GO:0009039; F:urease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR CDD; cd00390; Urease_gamma; 1.
DR Gene3D; 3.30.280.10; -; 1.
DR HAMAP; MF_00739; Urease_gamma; 1.
DR InterPro; IPR012010; Urease_gamma.
DR InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR InterPro; IPR036463; Urease_gamma_sf.
DR Pfam; PF00547; Urease_gamma; 1.
DR PIRSF; PIRSF001223; Urease_gamma; 1.
DR SUPFAM; SSF54111; SSF54111; 1.
DR TIGRFAMs; TIGR00193; urease_gam; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Hydrolase; Reference proteome.
FT CHAIN 1..100
FT /note="Urease subunit gamma"
FT /id="PRO_0000098011"
SQ SEQUENCE 100 AA; 11085 MW; 8A492D5A2662DCF6 CRC64;
MELTPREKDK LLLFTAALLA ERRLARGLKL NYPESVALIS AFIMEGARDG KSVAALMEEG
RHVLSREQVM EGIPEMIPDI QVEATFPDGS KLVTVHNPII
