ID   URE3_STAS1              Reviewed;         100 AA.
AC   Q4A0J3;
DT   22-NOV-2005, integrated into UniProtKB/Swiss-Prot.
DT   13-SEP-2005, sequence version 1.
DT   25-MAY-2022, entry version 109.
DE   RecName: Full=Urease subunit gamma {ECO:0000255|HAMAP-Rule:MF_00739};
DE            EC=3.5.1.5 {ECO:0000255|HAMAP-Rule:MF_00739};
DE   AltName: Full=Urea amidohydrolase subunit gamma {ECO:0000255|HAMAP-Rule:MF_00739};
GN   Name=ureA {ECO:0000255|HAMAP-Rule:MF_00739}; OrderedLocusNames=SSP0265;
OS   Staphylococcus saprophyticus subsp. saprophyticus (strain ATCC 15305 / DSM
OS   20229 / NCIMB 8711 / NCTC 7292 / S-41).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=342451;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15305 / DSM 20229 / NCIMB 8711 / NCTC 7292 / S-41;
RX   PubMed=16135568; DOI=10.1073/pnas.0502950102;
RA   Kuroda M., Yamashita A., Hirakawa H., Kumano M., Morikawa K., Higashide M.,
RA   Maruyama A., Inose Y., Matoba K., Toh H., Kuhara S., Hattori M., Ohta T.;
RT   "Whole genome sequence of Staphylococcus saprophyticus reveals the
RT   pathogenesis of uncomplicated urinary tract infection.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:13272-13277(2005).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-4, CATALYTIC ACTIVITY, AND BIOPHYSICOCHEMICAL
RP   PROPERTIES.
RX   PubMed=8042901; DOI=10.1007/bf00288948;
RA   Schaefer U.K., Kaltwasser H.;
RT   "Urease from Staphylococcus saprophyticus: purification, characterization
RT   and comparison to Staphylococcus xylosus urease.";
RL   Arch. Microbiol. 161:393-399(1994).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2 H(+) + H2O + urea = CO2 + 2 NH4(+); Xref=Rhea:RHEA:20557,
CC         ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16199,
CC         ChEBI:CHEBI:16526, ChEBI:CHEBI:28938; EC=3.5.1.5;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00739,
CC         ECO:0000269|PubMed:8042901};
CC   -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC       Kinetic parameters:
CC         KM=9.5 mM for urea (at 37 degrees Celsius and pH 7.0)
CC         {ECO:0000269|PubMed:8042901};
CC         Vmax=1.979 mmol/min/mg enzyme {ECO:0000269|PubMed:8042901};
CC       pH dependence:
CC         Optimum pH is 7.0 in phosphate buffer. {ECO:0000269|PubMed:8042901};
CC       Temperature dependence:
CC         Optimum temperature is 55 degrees Celsius.
CC         {ECO:0000269|PubMed:8042901};
CC   -!- PATHWAY: Nitrogen metabolism; urea degradation; CO(2) and NH(3) from
CC       urea (urease route): step 1/1. {ECO:0000255|HAMAP-Rule:MF_00739}.
CC   -!- SUBUNIT: Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha)
CC       subunits. Three heterotrimers associate to form the active enzyme.
CC       {ECO:0000255|HAMAP-Rule:MF_00739}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00739}.
CC   -!- SIMILARITY: Belongs to the urease gamma subunit family.
CC       {ECO:0000255|HAMAP-Rule:MF_00739}.
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DR   EMBL; AP008934; BAE17410.1; -; Genomic_DNA.
DR   RefSeq; WP_011302251.1; NZ_MTGA01000037.1.
DR   AlphaFoldDB; Q4A0J3; -.
DR   SMR; Q4A0J3; -.
DR   STRING; 342451.SSP0265; -.
DR   EnsemblBacteria; BAE17410; BAE17410; SSP0265.
DR   KEGG; ssp:SSP0265; -.
DR   eggNOG; COG0831; Bacteria.
DR   HOGENOM; CLU_145825_1_0_9; -.
DR   OMA; MQLTPHE; -.
DR   OrthoDB; 1826954at2; -.
DR   UniPathway; UPA00258; UER00370.
DR   Proteomes; UP000006371; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:InterPro.
DR   GO; GO:0009039; F:urease activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0043419; P:urea catabolic process; IEA:UniProtKB-UniPathway.
DR   CDD; cd00390; Urease_gamma; 1.
DR   Gene3D; 3.30.280.10; -; 1.
DR   HAMAP; MF_00739; Urease_gamma; 1.
DR   InterPro; IPR012010; Urease_gamma.
DR   InterPro; IPR002026; Urease_gamma/gamma-beta_su.
DR   InterPro; IPR036463; Urease_gamma_sf.
DR   Pfam; PF00547; Urease_gamma; 1.
DR   PIRSF; PIRSF001223; Urease_gamma; 1.
DR   SUPFAM; SSF54111; SSF54111; 1.
DR   TIGRFAMs; TIGR00193; urease_gam; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Direct protein sequencing; Hydrolase; Reference proteome.
FT   CHAIN           1..100
FT                   /note="Urease subunit gamma"
FT                   /id="PRO_0000098046"
SQ   SEQUENCE   100 AA;  11313 MW;  1A080F1B5E7F718B CRC64;
     MHFTQREQDK LMLVIAADLA RRRQQRGLKL NYPEAVAIIS FELLEGARDG KTVAELMSYG
     KQILNEDDVM EGVADMLTEM EIEATFPDGT KLITVHHPIV