URED1_BRUSU
ID URED1_BRUSU Reviewed; 280 AA.
AC Q8G2Q1; G0KBW6;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 25-MAY-2022, entry version 76.
DE RecName: Full=Urease accessory protein UreD 1 {ECO:0000255|HAMAP-Rule:MF_01384};
GN Name=ureD1 {ECO:0000255|HAMAP-Rule:MF_01384}; Synonyms=ureD-1;
GN OrderedLocusNames=BR0267, BS1330_I0268;
OS Brucella suis biovar 1 (strain 1330).
OC Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX NCBI_TaxID=204722;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=12271122; DOI=10.1073/pnas.192319099;
RA Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT "The Brucella suis genome reveals fundamental similarities between animal
RT and plant pathogens and symbionts.";
RL Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=1330;
RX PubMed=22038969; DOI=10.1128/jb.06181-11;
RA Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT "Revised genome sequence of Brucella suis 1330.";
RL J. Bacteriol. 193:6410-6410(2011).
RN [3]
RP OPERON DISRUPTION, AND ROLE IN VIRULENCE.
RC STRAIN=1330;
RX PubMed=17578575; DOI=10.1186/1471-2180-7-57;
RA Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M.,
RA Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N.,
RA Schurig G.G., Boyle S.M.;
RT "Brucella suis urease encoded by ure1 but not ure2 is necessary for
RT intestinal infection of BALB/c mice.";
RL BMC Microbiol. 7:57-57(2007).
CC -!- FUNCTION: Required for maturation of urease via the functional
CC incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC Rule:MF_01384}.
CC -!- FUNCTION: Disrupting the ure1 operon causes loss of urease activity,
CC decreased resistance to low pH killing in vitro and decreased pathogen
CC survival when inoculated in BALB/c mice by gavage.
CC {ECO:0000269|PubMed:17578575}.
CC -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01384}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC Rule:MF_01384}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AE014291; AAN29216.1; -; Genomic_DNA.
DR EMBL; CP002997; AEM17629.1; -; Genomic_DNA.
DR RefSeq; WP_004689446.1; NZ_KN046804.1.
DR AlphaFoldDB; Q8G2Q1; -.
DR SMR; Q8G2Q1; -.
DR EnsemblBacteria; AEM17629; AEM17629; BS1330_I0268.
DR GeneID; 45123773; -.
DR GeneID; 55590046; -.
DR KEGG; bms:BR0267; -.
DR KEGG; bsi:BS1330_I0268; -.
DR PATRIC; fig|204722.21.peg.1749; -.
DR HOGENOM; CLU_056339_2_0_5; -.
DR OMA; MARFCAQ; -.
DR PhylomeDB; Q8G2Q1; -.
DR Proteomes; UP000007104; Chromosome I.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR HAMAP; MF_01384; UreD; 1.
DR InterPro; IPR002669; UreD.
DR PANTHER; PTHR33643; PTHR33643; 1.
DR Pfam; PF01774; UreD; 1.
PE 3: Inferred from homology;
KW Chaperone; Cytoplasm; Nickel insertion; Virulence.
FT CHAIN 1..280
FT /note="Urease accessory protein UreD 1"
FT /id="PRO_0000340427"
SQ SEQUENCE 280 AA; 30230 MW; 2555CA77107D953D CRC64;
MLIINDNNLS GLSLQRVNGT GELSVQFKDG RSRISRLYQE GAAKIRMPQA VTGPLEAILI
NTSGGLTGGD RLKWDVALDD GASAVITTQA CERIYRSGGG EARIATRLKA AKGTRLAWLP
QETILFNRSI LSRRLDVELE EGAQMLVVEA TVFGRLAMGE RVVAARFADR WRVRLGGRVI
HAEEFRLGPD VGAELQAPAV AGGACAMATV LMVCEQAGRH LETARAIIGE EGGCSLWRVG
KASKLVVRLY APDSYALRRR LCPLVALLNG KAGLPKVWTI