ID   URED2_BRUA2             Reviewed;         304 AA.
AC   Q2YQD5;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   07-FEB-2006, sequence version 1.
DT   25-MAY-2022, entry version 64.
DE   RecName: Full=Urease accessory protein UreD 2 {ECO:0000255|HAMAP-Rule:MF_01384};
GN   Name=ureD2 {ECO:0000255|HAMAP-Rule:MF_01384}; Synonyms=ureD-2;
GN   OrderedLocusNames=BAB1_1382;
OS   Brucella abortus (strain 2308).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=359391;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=2308;
RX   PubMed=16299333; DOI=10.1128/iai.73.12.8353-8361.2005;
RA   Chain P.S., Comerci D.J., Tolmasky M.E., Larimer F.W., Malfatti S.A.,
RA   Vergez L.M., Aguero F., Land M.L., Ugalde R.A., Garcia E.;
RT   "Whole-genome analyses of speciation events in pathogenic Brucellae.";
RL   Infect. Immun. 73:8353-8361(2005).
RN   [2]
RP   LACK OF ROLE IN VIRULENCE.
RX   PubMed=17101645; DOI=10.1128/iai.01244-06;
RA   Sangari F.J., Seoane A., Rodriguez M.C., Aguero J., Garcia Lobo J.M.;
RT   "Characterization of the urease operon of Brucella abortus and assessment
RT   of its role in virulence of the bacterium.";
RL   Infect. Immun. 75:774-780(2007).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
CC   -!- FUNCTION: Disrupting the ure2 operon has no effect on urease activity
CC       or pathogen survival in BALB/c mice when administered orally.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
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DR   EMBL; AM040264; CAJ11338.1; -; Genomic_DNA.
DR   RefSeq; WP_002964474.1; NZ_KN046823.1.
DR   AlphaFoldDB; Q2YQD5; -.
DR   SMR; Q2YQD5; -.
DR   STRING; 359391.BAB1_1382; -.
DR   EnsemblBacteria; CAJ11338; CAJ11338; BAB1_1382.
DR   GeneID; 3789050; -.
DR   KEGG; bmf:BAB1_1382; -.
DR   PATRIC; fig|359391.11.peg.832; -.
DR   HOGENOM; CLU_056339_1_0_5; -.
DR   OMA; IPFENAH; -.
DR   PhylomeDB; Q2YQD5; -.
DR   Proteomes; UP000002719; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   HAMAP; MF_01384; UreD; 1.
DR   InterPro; IPR002669; UreD.
DR   PANTHER; PTHR33643; PTHR33643; 1.
DR   Pfam; PF01774; UreD; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT   CHAIN           1..304
FT                   /note="Urease accessory protein UreD 2"
FT                   /id="PRO_0000340420"
SQ   SEQUENCE   304 AA;  33915 MW;  8373267BF3E80858 CRC64;
     MLGKARELAN YQDEPEQLPT GSFGKNAFLR LGFERRPERT VLATLHRRAP LIVQQALYWD
     EGMPTLPCVS IISNAGGILQ GDRYAIEIDL EPDTQAHVTT QSATRIQEMD ANFATQTQTQ
     TITLGANSYL EYIPHPIIPH KHSRFVQQTE VTIHPTATLI YSEVLMAGRK YYGTGELFHY
     DLFSSKFHAA HTDGTSLFTE KFIVEPARGN VSRLGAMGSF HVFGNLILLT PKTHADRLFE
     TIDPVFDMDE GIAWGASRLP NDAGLLFKVL GMESAPVRAA IRKIWEAARQ EVTGASLPEN
     FLWA