ID   URED2_BRUSU             Reviewed;         302 AA.
AC   Q8FZV8; G0KB47;
DT   10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2003, sequence version 1.
DT   25-MAY-2022, entry version 73.
DE   RecName: Full=Urease accessory protein UreD 2 {ECO:0000255|HAMAP-Rule:MF_01384};
GN   Name=ureD2 {ECO:0000255|HAMAP-Rule:MF_01384}; Synonyms=ureD-2;
GN   OrderedLocusNames=BR1362, BS1330_I1357;
OS   Brucella suis biovar 1 (strain 1330).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales;
OC   Brucellaceae; Brucella/Ochrobactrum group; Brucella.
OX   NCBI_TaxID=204722;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=12271122; DOI=10.1073/pnas.192319099;
RA   Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F.,
RA   Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J.,
RA   Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R.,
RA   Nelson W.C., Ayodeji B., Kraul M., Shetty J., Malek J.A., Van Aken S.E.,
RA   Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L.,
RA   Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.;
RT   "The Brucella suis genome reveals fundamental similarities between animal
RT   and plant pathogens and symbionts.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=1330;
RX   PubMed=22038969; DOI=10.1128/jb.06181-11;
RA   Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.;
RT   "Revised genome sequence of Brucella suis 1330.";
RL   J. Bacteriol. 193:6410-6410(2011).
RN   [3]
RP   OPERON DISRUPTION, AND LACK OF ROLE IN VIRULENCE.
RC   STRAIN=1330;
RX   PubMed=17578575; DOI=10.1186/1471-2180-7-57;
RA   Bandara A.B., Contreras A., Contreras-Rodriguez A., Martins A.M.,
RA   Dobrean V., Poff-Reichow S., Rajasekaran P., Sriranganathan N.,
RA   Schurig G.G., Boyle S.M.;
RT   "Brucella suis urease encoded by ure1 but not ure2 is necessary for
RT   intestinal infection of BALB/c mice.";
RL   BMC Microbiol. 7:57-57(2007).
CC   -!- FUNCTION: Required for maturation of urease via the functional
CC       incorporation of the urease nickel metallocenter. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
CC   -!- FUNCTION: Disrupting the ure2 operon has no effect on urease activity,
CC       or pathogen survival in BALB/c mice when inoculated by gavage, but
CC       confers slightly enhanced resistance to low pH killing in vitro.
CC   -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC       hydrolysis-dependent molecular chaperone, activating the urease
CC       apoprotein by helping to assemble the nickel containing metallocenter
CC       of UreC. The UreE protein probably delivers the nickel.
CC       {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC   -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01384}.
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DR   EMBL; AE014291; AAN30276.1; -; Genomic_DNA.
DR   EMBL; CP002997; AEM18693.1; -; Genomic_DNA.
DR   PIR; AE3332; AE3332.
DR   RefSeq; WP_002967786.1; NZ_KN046804.1.
DR   AlphaFoldDB; Q8FZV8; -.
DR   SMR; Q8FZV8; -.
DR   EnsemblBacteria; AEM18693; AEM18693; BS1330_I1357.
DR   GeneID; 45052382; -.
DR   KEGG; bms:BR1362; -.
DR   KEGG; bsi:BS1330_I1357; -.
DR   PATRIC; fig|204722.21.peg.837; -.
DR   HOGENOM; CLU_056339_1_0_5; -.
DR   OMA; IPFENAH; -.
DR   PhylomeDB; Q8FZV8; -.
DR   Proteomes; UP000007104; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR   HAMAP; MF_01384; UreD; 1.
DR   InterPro; IPR002669; UreD.
DR   PANTHER; PTHR33643; PTHR33643; 1.
DR   Pfam; PF01774; UreD; 1.
PE   3: Inferred from homology;
KW   Chaperone; Cytoplasm; Nickel insertion.
FT   CHAIN           1..302
FT                   /note="Urease accessory protein UreD 2"
FT                   /id="PRO_0000340428"
SQ   SEQUENCE   302 AA;  33685 MW;  BD228D3B2E437E06 CRC64;
     MLGKARELAN YQDEPEQLPT GSFGKNAFLR LGFERRPERT VLATLHRRAP LIVQQALYWD
     EGMPTLPCVS IISNAGGILQ GDRYAIEIDL EPDTQAHVTT QSATRIQEMD ANFATQTQTI
     TLGANSYLEY IPHPIIPHKH SRFVQQTEVT IHPTATLIYS EVLMAGRKYY GTGELFHYDL
     FSSKFHAAHT DGTSLFTEKF IVEPARGNVS RLGAMGSFHV FGNLILLTPK THADRLFETI
     DPVFDMDEGI AWGASRLPND AGLLFKVLGM ESAPVRAAIR KIWEAARQEV TGASLPENFL
     WA