URED_CORGL
ID URED_CORGL Reviewed; 283 AA.
AC Q79VJ0; Q9L416; Q9RHM0;
DT 10-JUN-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-JUL-2004, sequence version 1.
DT 03-AUG-2022, entry version 97.
DE RecName: Full=Urease accessory protein UreD {ECO:0000255|HAMAP-Rule:MF_01384};
GN Name=ureD1 {ECO:0000255|HAMAP-Rule:MF_01384};
GN OrderedLocusNames=Cgl0090, cg0119;
OS Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 /
OS JCM 1318 / LMG 3730 / NCIMB 10025).
OC Bacteria; Actinobacteria; Corynebacteriales; Corynebacteriaceae;
OC Corynebacterium.
OX NCBI_TaxID=196627;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC STRAIN=ATCC 13869 / DSMZ 1412 / NCIMB 9567;
RX PubMed=11328647; DOI=10.3109/10425170009033989;
RA Puskas L.G., Inui M., Yukawa H.;
RT "Structure of the urease operon of Corynebacterium glutamicum.";
RL DNA Seq. 11:383-394(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROBABLE OPERON STRUCTURE, AND
RP DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=10930756; DOI=10.1111/j.1574-6968.2000.tb09248.x;
RA Nolden L., Beckers G., Moeckel B., Pfefferle W., Nampoothiri K.M.,
RA Kraemer R., Burkovski A.;
RT "Urease of Corynebacterium glutamicum: organization of corresponding genes
RT and investigation of activity.";
RL FEMS Microbiol. Lett. 189:305-310(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12743753; DOI=10.1007/s00253-003-1328-1;
RA Ikeda M., Nakagawa S.;
RT "The Corynebacterium glutamicum genome: features and impacts on
RT biotechnological processes.";
RL Appl. Microbiol. Biotechnol. 62:99-109(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB
RC 10025;
RX PubMed=12948626; DOI=10.1016/s0168-1656(03)00154-8;
RA Kalinowski J., Bathe B., Bartels D., Bischoff N., Bott M., Burkovski A.,
RA Dusch N., Eggeling L., Eikmanns B.J., Gaigalat L., Goesmann A.,
RA Hartmann M., Huthmacher K., Kraemer R., Linke B., McHardy A.C., Meyer F.,
RA Moeckel B., Pfefferle W., Puehler A., Rey D.A., Rueckert C., Rupp O.,
RA Sahm H., Wendisch V.F., Wiegraebe I., Tauch A.;
RT "The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its
RT impact on the production of L-aspartate-derived amino acids and vitamins.";
RL J. Biotechnol. 104:5-25(2003).
CC -!- FUNCTION: Probably acts in the maturation of urease via the functional
CC incorporation of the urease nickel metallocenter (By similarity).
CC Required for urease expression. {ECO:0000250}.
CC -!- SUBUNIT: UreD, UreF and UreG form a complex that acts as a GTP-
CC hydrolysis-dependent molecular chaperone, activating the urease
CC apoprotein by helping to assemble the nickel containing metallocenter
CC of UreC. The UreE protein probably delivers the nickel.
CC {ECO:0000255|HAMAP-Rule:MF_01384}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01384}.
CC -!- INDUCTION: By urea and nitrogen starvation.
CC {ECO:0000269|PubMed:11328647}.
CC -!- DISRUPTION PHENOTYPE: Cells are urease negative and are no longer able
CC to transport urea. {ECO:0000269|PubMed:10930756}.
CC -!- SIMILARITY: Belongs to the UreD family. {ECO:0000255|HAMAP-
CC Rule:MF_01384}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA88558.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AB029154; BAA88558.1; ALT_FRAME; Genomic_DNA.
DR EMBL; AJ251883; CAB81941.1; -; Genomic_DNA.
DR EMBL; BA000036; BAB97483.1; -; Genomic_DNA.
DR EMBL; BX927148; CAF18658.1; -; Genomic_DNA.
DR RefSeq; NP_599342.1; NC_003450.3.
DR RefSeq; WP_011013381.1; NC_006958.1.
DR AlphaFoldDB; Q79VJ0; -.
DR SMR; Q79VJ0; -.
DR STRING; 196627.cg0119; -.
DR KEGG; cgb:cg0119; -.
DR KEGG; cgl:Cgl0090; -.
DR PATRIC; fig|196627.13.peg.91; -.
DR eggNOG; COG0829; Bacteria.
DR HOGENOM; CLU_056339_5_0_11; -.
DR OMA; CQHIIVH; -.
DR Proteomes; UP000000582; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0016151; F:nickel cation binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006807; P:nitrogen compound metabolic process; IEA:InterPro.
DR HAMAP; MF_01384; UreD; 1.
DR InterPro; IPR002669; UreD.
DR PANTHER; PTHR33643; PTHR33643; 1.
DR Pfam; PF01774; UreD; 1.
PE 2: Evidence at transcript level;
KW Chaperone; Cytoplasm; Nickel insertion; Reference proteome.
FT CHAIN 1..283
FT /note="Urease accessory protein UreD"
FT /id="PRO_0000340448"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 222
FT /note="L -> I (in Ref. 1; BAA88558)"
FT /evidence="ECO:0000305"
FT CONFLICT 241
FT /note="L -> E (in Ref. 1; BAA88558)"
FT /evidence="ECO:0000305"
FT CONFLICT 258
FT /note="I -> V (in Ref. 1; BAA88558)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 283 AA; 31646 MW; BE1B05AE876E7417 CRC64;
MTQTQPVGTL RLTIDDQGPQ GQSRAVEQFH QGALRVIRPH YLDDSGQVCY TIIAIGGGYL
GGDVYEQQFT IKDNAKALIT TQSATKIYRT PQGPATQHTE INVGENAVLE YLADQTIAYR
EATYHQFTKV ALHPSATFVM SEQITPGWHP DGKHFAYDEM RLHTEITDST TGRLVLLDNL
LLRPDSREGS FGWTEQYTHS GQMIVMGEGV DKQLVAELNE QLAAHPDVYG AVNFLSAPGT
LLRGFIARTL SNRTEELINL HEHIASLLRG RWRGQEPVNL RKY
